PurposeThis plasmid contains expression cassette for sgRNA targeting tdTomato.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47871||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3851
- Total vector size (bp) 4301
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)151
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note that this plasmid is only compatible with Neisseria meningitidis CRISPR systems.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSTBlue-U6-sgRNA(tdTomato) was a gift from Erik Sontheimer & James Thomson (Addgene plasmid # 47871 ; http://n2t.net/addgene:47871 ; RRID:Addgene_47871)
For your References section:Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA. Proc Natl Acad Sci U S A. 2013 Aug 12. 10.1073/pnas.1313587110 PubMed 23940360