|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47872||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 8501
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSimpleII-NmCas9-FLAG was a gift from Erik Sontheimer & James Thomson (Addgene plasmid # 47872 ; http://n2t.net/addgene:47872 ; RRID:Addgene_47872)
For your References section:Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA. Proc Natl Acad Sci U S A. 2013 Aug 12. 10.1073/pnas.1313587110 PubMed 23940360