315,948 results

1. Reckleen_2_SpGCas9

   Plasmid

   #233457

   Purpose

   Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

   Depositor

   Anke Becker

   Article

   Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

   Insert

   Level 2 RECKLEEN plasmid
   

   Use

   CRISPR

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDC32B-NbmiR482aTS-B/c

   Plasmid

   #227967

   Purpose

   Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Inserts

   B/c
   NbmiR482aTS
   

   Expression

   Plant

   Promoter

   35S and None

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2188-PrARG8-GFP::URA3

   Plasmid

   #216512

   Purpose

   Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

   Depositor

   Juha Saarikangas

   Article

   Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

   Insert

   PrARG8-yeGFP
   

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Promoter

   ARG8

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB2197-PrGNP1-mK2-NatMX

   Plasmid

   #216513

   Purpose

   Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

   Depositor

   Juha Saarikangas

   Article

   Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

   Insert

   PrGnp1-mKate2
   

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Promoter

   GNP1

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB2188-PrARG8-GFP::URA3

   Plasmid

   #216512

   Purpose

   Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

   Depositor

   Juha Saarikangas

   Article

   Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

   Insert

   PrARG8-yeGFP
   

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Promoter

   ARG8

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCfB2197-PrGNP1-mK2-NatMX

   Plasmid

   #216513

   Purpose

   Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

   Depositor

   Juha Saarikangas

   Article

   Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

   Insert

   PrGnp1-mKate2
   

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Promoter

   GNP1

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-mGold2t-Lysosome-N-20

   Plasmid

   #231778

   Purpose

   Visualization of lysosome in mammalian cells using mGold2t

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t-Lysosome-N-20 (Lamp1 Rat)
   

   Tags

   mGold2s

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMK195

   Plasmid

   #226818

   Purpose

   Integrates KanR into NS2, 1250 bp homology, two homologous HIP1 sites in each of the right and left homology arms

   Depositor

   David Nielsen

   Article

   Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-GST-8

   Plasmid

   #228990

   Purpose

   For bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-8
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Epac-S-H326

    Plasmid

    #228242

    Purpose

    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Epac-S-H327

    Plasmid

    #228243

    Purpose

    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Epac-S-H328

    Plasmid

    #228244

    Purpose

    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Epac-S-H329

    Plasmid

    #228245

    Purpose

    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Epac-S-H332

    Plasmid

    #228250

    Purpose

    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUASTattB-degradALFA

    Plasmid

    #219663

    Purpose

    Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    degradALFA
    

    Expression

    Insect

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWN172

    Plasmid

    #215291

    Purpose

    Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    J23113

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWN172

    Plasmid

    #215291

    Purpose

    Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    J23113

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EC70189

    Plasmid

    #209450

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    HvU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EC70189

    Plasmid

    #209450

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    HvU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDisplay-GRAB_g5-HT3.0mut

    Plasmid

    #208712

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cells

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge