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C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector.

Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F
Science. 2016 Jun 2. pii: aaf5573. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
79150pC001 - huLshC2C2-MBP for bacterial expressionExpresses human codon-optimized LshC2c2 for purification in E. coli
79151pC002 - LshC2C2 locus into pACYC184Endogenous LshC2c2 locus cloned into pACYC184
79152pC003 - LshC2C2 locus into pACYC184 for spacer cloningLshC2c2 locus in pACYC184 with BsaI sites for spacer cloning
79153pC004 - PFS library in Beta-lactamase gene Target PlasmidPFS library inserted at the 5' of the Beta-lactamase gene for screening PFS preference
79156pC007 - pRFP constitutive expressionRFP constitutively expressed
79157pC008 - pBR322 with tetR-inducible RFPinducible RFP expression vector

Antibodies from Article