C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector.
Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F
Science. 2016 Jun 2. pii: aaf5573. PubMed Article
Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F
Science. 2016 Jun 2. pii: aaf5573. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79150 | pC001 - huLshC2C2-MBP for bacterial expression | Expresses human codon-optimized LshC2c2 for purification in E. coli |
| 79151 | pC002 - LshC2C2 locus into pACYC184 | Endogenous LshC2c2 locus cloned into pACYC184 |
| 79152 | pC003 - LshC2C2 locus into pACYC184 for spacer cloning | LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning |
| 79153 | pC004 - PFS library in Beta-lactamase gene Target Plasmid | PFS library inserted at the 5' of the Beta-lactamase gene for screening PFS preference |
| 79156 | pC007 - pRFP constitutive expression | RFP constitutively expressed |
| 79157 | pC008 - pBR322 with tetR-inducible RFP | inducible RFP expression vector |