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pC003 - LshC2C2 locus into pACYC184 for spacer cloning
(Plasmid #79152)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79152 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    STBL3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LshC2c2 locus
  • Species
    Leptotrichia shahii
  • Mutation
    *

Resource Information

Depositor Comments

*There is a premature stop int he Cas2 protein, removing the final 22 aa's (MKVLAGEVTMGEKISQNKILEF). From the author: "The Cas1/Cas2 are not necessary for the purposes of the plasmid as we have shown that C2c2 is all that is necessary for RNA targeting. [Investigators] should use pC001 if they’re actually interested in the Cas proteins."

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC003 - LshC2C2 locus into pACYC184 for spacer cloning was a gift from Feng Zhang (Addgene plasmid # 79152 ; http://n2t.net/addgene:79152 ; RRID:Addgene_79152)
  • For your References section:

    C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F. Science. 2016 Jun 2. pii: aaf5573. 10.1126/science.aaf5573 PubMed 27256883