pC003 - LshC2C2 locus into pACYC184 for spacer cloning
PurposeLshC2c2 locus in pACYC184 with BsaI sites for spacer cloning
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79152||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4723
- Total vector size (bp) 10444
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameLshC2c2 locus
*There is a premature stop int he Cas2 protein, removing the final 22 aa's (MKVLAGEVTMGEKISQNKILEF). From the author: "The Cas1/Cas2 are not necessary for the purposes of the plasmid as we have shown that C2c2 is all that is necessary for RNA targeting. [Investigators] should use pC001 if they’re actually interested in the Cas proteins."
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pC003 - LshC2C2 locus into pACYC184 for spacer cloning was a gift from Feng Zhang (Addgene plasmid # 79152 ; http://n2t.net/addgene:79152 ; RRID:Addgene_79152)
For your References section:C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F. Science. 2016 Jun 2. pii: aaf5573. 10.1126/science.aaf5573 PubMed 27256883