Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pLVX-UbC-rtTA-Ngn2:2A:Ascl1
(Plasmid #127289)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127289 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLVX-tight-puro and pLVX-rtTA(advanced)
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 9766
  • Total vector size (bp) 11362
  • Modifications to backbone
    vector contains both the rtTA(advanced) derived from pLVX-rtTA(advanced) (Clontech) driven by the UbC promoter, as well as Ngn2:2A:Ascl1 driven by the TREtight promoter
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Ngn2:2A:Ascl1
  • Alt name
    Neurogenin 2, NEUROG2
  • Alt name
    Mash1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
    NM_004316.4 NM_024019.4
  • Entrez Gene
    ASCL1 (a.k.a. ASH1, HASH1, MASH1, bHLHa46)
  • Entrez Gene
    NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
  • Tag / Fusion Protein
    • Ngn2 and Ascl1 linked via 2A

Gene/Insert 2

  • Gene/Insert name
    Ngn2:2A:Ascl1
  • Alt name
    Mash1
  • Alt name
    Neurogenin 2, NEUROG2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
    NM_004316.4 NM_024019.4
  • Entrez Gene
    ASCL1 (a.k.a. ASH1, HASH1, MASH1, bHLHa46)
  • Entrez Gene
    NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-UbC-rtTA-Ngn2:2A:Ascl1 was a gift from Fred Gage (Addgene plasmid # 127289 ; http://n2t.net/addgene:127289 ; RRID:Addgene_127289)
  • For your References section:

    Chemical modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Herdy J, Schafer S, Kim Y, Ansari Z, Zangwill D, Ku M, Paquola A, Lee H, Mertens J, Gage FH. Elife. 2019 May 17;8. pii: 41356. doi: 10.7554/eLife.41356. 10.7554/eLife.41356 PubMed 31099332