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pLVX-UbC-rtTA-Ngn2:2A:EGFP
(Plasmid #127288)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127288 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLVX-tight-puro and pLVX-rtTA(advanced)
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 9766
  • Total vector size (bp) 11362
  • Modifications to backbone
    vector contains both the rtTA(advanced) derived from pLVX-rtTA(advanced) (Clontech) driven by the UbC promoter, as well as Ngn2:2A:EGFP driven by the TREtight promoter
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ngn2:2A:Ascl1
  • Alt name
    Neurogenin 2, NEUROG2
  • Alt name
    EGFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
    NM_024019.4
  • Entrez Gene
    NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
  • Tag / Fusion Protein
    • Ngn2 and EGFP linked via 2A

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-UbC-rtTA-Ngn2:2A:EGFP was a gift from Fred Gage (Addgene plasmid # 127288 ; http://n2t.net/addgene:127288 ; RRID:Addgene_127288)
  • For your References section:

    Pathological priming causes developmental gene network heterochronicity in autistic subject-derived neurons. Schafer ST, Paquola ACM, Stern S, Gosselin D, Ku M, Pena M, Kuret TJM, Liyanage M, Mansour AA, Jaeger BN, Marchetto MC, Glass CK, Mertens J, Gage FH. Nat Neurosci. 2019 Feb;22(2):243-255. doi: 10.1038/s41593-018-0295-x. Epub 2019 Jan 7. 10.1038/s41593-018-0295-x PubMed 30617258