Purposeexpresses tdTomato fluorescent protein under the CAG promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83029||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerCepko Lab
- Backbone size w/o insert (bp) 5551
- Total vector size (bp) 6261
Modifications to backboneCFP removed using AgeI and NotI
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)1441
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pCAG-CFP was used for the backbone. CFP was excised with AgeI and NotI, and tdTomato was inserted using the same restriction sites. These plasmids were also used in the following publication "Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration. Lin TV, Hsieh L, Kimura T, Malone TJ, Bordey A. Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11330-11335."
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-tdTomato was a gift from Angelique Bordey (Addgene plasmid # 83029 ; http://n2t.net/addgene:83029 ; RRID:Addgene_83029)
For your References section:miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons. Pathania M, Torres-Reveron J, Yan L, Kimura T, Lin TV, Gordon V, Teng ZQ, Zhao X, Fulga TA, Van Vactor D, Bordey A. PLoS One. 2012;7(5):e38174. doi: 10.1371/journal.pone.0038174. Epub 2012 May 31. 10.1371/journal.pone.0038174 PubMed 22693596