163,905 results

1. Lamp1-mScarlet-I

   Plasmid

   #98827

   Purpose

   In vivo visualization of lysosomes (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   Lamp1
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

   Plasmid

   #209730

   Purpose

   Expresses presynaptic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-VLDLR

   Plasmid

   #116802

   Purpose

   Lentiviral expression of VLDLR

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   VLDLR (VLDLR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF5B-FRT-AP-Sstr3-GFP-DEST

   Plasmid

   #49098

   Purpose

   Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

   Depositor

   Maxence Nachury

   Article

   Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

   Insert

   Sstr3 (Sstr3 Mouse)
   

   Use

   Tags

   GFP and acceptor peptide (AP)

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Nov. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD24-sfGFPx2

   Plasmid

   #51559

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

   Depositor

   Francisco Malagon, Sankar Adhya

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Use

   Tags

   GLESTCRHASLAVLADERRFSA and MARARA

   Expression

   Bacterial

   Mutation

   Contains additonal aa in the N and C terminus (in…

   Promoter

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCTcon2

   Plasmid

   #41843

   Depositor

   Dane Wittrup

   Article

   Chao et al Nat Protoc. 2006;1(2):755-68.

   Insert

   CD20
   

   Use

   Tags

   HA and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV 3xFLAG-GABARAPL2 WT

   Plasmid

   #123103

   Purpose

   Expresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX307 P2A GFP Luciferase

   Plasmid

   #193674

   Purpose

   Constitutive lentiviral expression of Luciferase

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFVdelCME-mVenus

   Plasmid

   #179953

   Purpose

   pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   YFV-17D lacking C, prM, E proteins but with mVenus
   

   Use

   Transneuronal tracing

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-OGG1

    Plasmid

    #215135

    Purpose

    Expresses mCherry-OGG1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    OGG1 (OGG1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-IP-HaloTag7-LC3

    Plasmid

    #184899

    Purpose

    Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-PITCh-GOLGA2

    Plasmid

    #207791

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-G-Flamp2

    Plasmid

    #192782

    Purpose

    green biosensor for cAMP in mammalian cells

    Depositor

    Jun Chu

    Article

    Liu et al Front Pharmacol. 2022 May 12;13:902290.

    Insert

    G-Flamp2
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+MLS-HyPer7

    Plasmid

    #136470

    Purpose

    Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Tandem mitochondrial targeting signal of cytochro…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AnkyrinB-GFP/pEGFP-N1

    Plasmid

    #197327

    Purpose

    Plasmid expressing an antigen targeting AnkyrinB

    Depositor

    Vann Bennett

    Insert

    AnkyrinB (Ank2 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMP71-hCD155

    Plasmid

    #118630

    Purpose

    human CD155 coding sequence subcloned into pMP71 retroviral plasmid

    Depositor

    Sébastien Walchli

    Article

    Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

    Insert

    CD155 (PVR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-6xHis-sfGFP

    Plasmid

    #199171

    Purpose

    used to express a superfolder GFP in E. coli for comparison to supercharged variants

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    superfolder GFP
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1/V5-His Snk/Plk2

    Plasmid

    #16015

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

20. MTCH2_pLX307

    Plasmid

    #98354

    Purpose

    Lentiviral expression of MTCH2

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MTCH2 (MTCH2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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