161,398 results

1. Mouse Chromatin Regulator Library

   Pooled Library

   #200011

   Purpose

   CRISPR KO library targeting a comprehensive set of genes with annotated function as chromatin regulators.

   Depositor

   Bradley Bernstein, Gabriel Griffin, Robert Manguso

   Article

   Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

2. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV1)

   Viral Prep

   #198511-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Promoter

   Syn

   Tags

   mScarlet (Cre-dependent)

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

3. pRT043-CLYBL-DDdcas9-VPH

   Plasmid

   #158091

   Purpose

   Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

   Depositor

   Martin Kampmann

   Article

   Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

   Insert

   CLYBL-CAG-DDdCas9VPH-T2A-GFP
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-VSVL

   Plasmid

   #64085

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene

   Depositor

   Ian Wickersham

   Insert

   VSV polymerase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCasAb-apr

   Plasmid

   #121998

   Purpose

   Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET MBP His6 LIC cloning vector (2Cc-T)

   Plasmid

   #37237

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #71236

   Purpose

   Express sgRNA and dCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A Puro
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-neo FlucDM EGFP

   Plasmid

   #90172

   Purpose

   EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

   Depositor

   Franz-Ulrich Hartl

   Article

   Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

   Insert

   FLuc EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R188Q, R261Q in FLuc

   Promoter

   Available since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGK1p-Csy4-pA (Construct 2)

   Plasmid

   #55196

   Purpose

   Expresses Csy4 in Mammalian cells for site-specific RNA processing

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   Csy4/Cas6
   

   Use

   Synthetic Biology

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458-mcherry

    Plasmid

    #161974

    Purpose

    Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    Cas9-t2a-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PuroR-T2A-eGFP

    Plasmid

    #234734

    Purpose

    Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PuroR-T2A-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-VAPB

    Plasmid

    #108126

    Purpose

    ER marker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:VAPB (VAPB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. hFerritinH

    Plasmid

    #122652

    Purpose

    Expression of hFerritinH in bacterial cells

    Depositor

    Trevor Douglas

    Insert

    ferritin heavy chain 1 (FTH1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

    Plasmid

    #55209

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 10xHis, MPB, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRIS-PITChv2-BSD-dTAG (BRD4)

    Plasmid

    #91792

    Purpose

    PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    BSD-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promoterless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-Coff/Fon-sRGECO (AAV8)

    Viral Prep

    #137128-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-sRGECO (#137128). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-sRGECO plasmid DNA. EF1a-driven, Flp-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    None

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-hSyn-GRAB_NE1h (AAV9)

    Viral Prep

    #123309-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NE1h (#123309). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NE1h plasmid DNA. hSyn-driven expression of GRAB-NE1h norepinephrine sensor in neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Promoter

    Syn

    Tags

    None

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

18. pHCMV_Bless

    Plasmid

    #207255

    Purpose

    Mammalian expression of Baboon Endogenous Retrovirus env protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    BaboonEndogenousRetrovirus_Rless_env
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCmini-iCAP-PHP.S

    Plasmid

    #103006

    Purpose

    non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    Synthetic construct isolate AAV-PHP.S VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-AAV-syn-jGCaMP7s-WPRE (AAV9)

    Viral Prep

    #104487-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Tags

    None

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only