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pPGKpuro
(Plasmid #11349)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11349 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM11
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3200
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pgk-1 5' and 3' regions
  • Alt name
    phosphoglycerate kinase-1
  • Alt name
    PGK puro
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1700
  • Entrez Gene
    Pgk1 (a.k.a. Pgk-1)

Cloning Information

Resource Information

Depositor Comments

Constructed by Peter Laird. See author's map. Includes 500 bp mouse PGK promoter, 700 bp puro, 460 bp PGK 3' end.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPGKpuro was a gift from Rudolf Jaenisch (Addgene plasmid # 11349 ; http://n2t.net/addgene:11349 ; RRID:Addgene_11349)
  • For your References section:

    Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes. Tucker KL, Beard C, Dausmann J, Jackson-Grusby L, Laird PW, Lei H, Li E, Jaenisch R. Genes Dev. 1996 Apr 15. 10(8):1008-20. 10.1101/gad.10.8.1008 PubMed 8608936