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pBAD/myc-his A Rv0712 (FGE)
(Plasmid #16132)


Item Catalog # Description Quantity Price (USD)
Plasmid 16132 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pBAD/myc-his A
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4081
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Formylglycine-generating enzyme
  • Alt name
    m tuberculosis FGE
  • Alt name
    mtb FGE
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
  • GenBank ID

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pBAD-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The gene encoding Mycobacterium tuberculosis FGE (Rv0712, encoding residues 2-299, GenBank accession NP_215226) was amplified from M. tuberculosis H37Rv genomic DNA and ligated into pBAD/myc-his A between the NcoI and XhoI restriction sites. The FGE gene was placed in frame with the start codon using QuikChange mutagenesis.
Please note that this information page previously listed the NcoI cloning site as not destroyed. The site was destroyed during cloning, however, and the page was updated on 6/16/08.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD/myc-his A Rv0712 (FGE) was a gift from Carolyn Bertozzi (Addgene plasmid # 16132 ; ; RRID:Addgene_16132)
  • For your References section:

    Introducing genetically encoded aldehydes into proteins. Carrico IS, Carlson BL, Bertozzi CR. Nat Chem Biol. 2007 Jun . 3(6):321-2. 10.1038/nchembio878 PubMed 17450134