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His-FGE
(Plasmid #75147)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 75147 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET14b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4671
  • Total vector size (bp) 5568
  • Modifications to backbone
    N/A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FGE
  • Alt name
    Formylglycine Generating Enzyme
  • Alt name
    MtFGE
  • Alt name
    Mycobacterium tuberculosis FGE
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    954
  • Mutation
    N/A
  • GenBank ID
    NP_215226
  • Promoter T7
  • Tags / Fusion Proteins
    • Histidine tag (N terminal on insert)
    • Thrombin (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-FGE was a gift from Richard Fishel (Addgene plasmid # 75147 ; http://n2t.net/addgene:75147 ; RRID:Addgene_75147)
  • For your References section:

    An Efficient Site-Specific Method for Irreversible Covalent Labeling of Proteins with a Fluorophore. Liu J, Hanne J, Britton BM, Shoffner M, Albers AE, Bennett J, Zatezalo R, Barfield R, Rabuka D, Lee JB, Fishel R. Sci Rep. 2015 Nov 19;5:16883. doi: 10.1038/srep16883. 10.1038/srep16883 PubMed 26582263