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pcDNA-4mtD3cpv
(Plasmid #36324)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36324 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7800
  • Modifications to backbone
    mitochondrial targeting sequence N-terminal to insert
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
  • Alt name
    D family cameleon
  • Species
    Synthetic
  • Insert Size (bp)
    2370
  • GenBank ID
    DQ479429.1
  • Promoter CMV
  • Tag / Fusion Protein
    • First 29 aa of human COX subunit 8a (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and circularly permuted Venus 173. D3cpv does not bind wild-type calmodulin. It has a Kd' of 0.6 uM.

See also Palmer & Tsien (2006) Nature Protocols.

The small discrepancy between the Addgene and depositor's sequence should not alter the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-4mtD3cpv was a gift from Amy Palmer & Roger Tsien (Addgene plasmid # 36324 ; http://n2t.net/addgene:36324 ; RRID:Addgene_36324)
  • For your References section:

    Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs. Palmer AE, Giacomello M, Kortemme T, Hires SA, Lev-Ram V, Baker D, Tsien RY. Chem Biol. 2006 May;13(5):521-30. 10.1016/j.chembiol.2006.03.007 PubMed 16720273