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pLenti SpBsmBI sgRNA Hygro

Plasmid

#62205

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

Depositor

Rene Maehr

Publication

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Publication

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLKO5.sgRNA.EFS.tRFP657

Plasmid

#57824

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP657

Use

CRISPR and Lentiviral

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Publication

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pDD104 (Peft-3::Cre)

Plasmid

#47551

Purpose

Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

Depositor

Bob Goldstein

Publication

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Insert

Cre Recombinase

Use

Cre/Lox

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pRSET-mSA

Plasmid

#39860

Depositor

Sheldon Park

Publication

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

6xHis and FLAG

Expression

Bacterial

Promoter

T7

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTARA

Plasmid

#31491

Depositor

Kathleen Matthews

Publication

Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

Insert

RNA polymerase

Expression

Bacterial

Mutation

N823D

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAVS1 hPGK-PuroR-pA donor

Plasmid

#22072

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PGK-Puro (PGK1 Human)

Use

Zfn donor

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pEZYmyc-His

Plasmid

#18701

Purpose

pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Publication

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Tags

Myc-His

Expression

Mammalian

Promoter

CMV

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCPRDEnv

Plasmid

#1732

Purpose

This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

Depositor

Garry Nolan

Insert

Gag-Pol and Rev

Expression

Mammalian

Available since

Oct. 24, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-hSyn-DIO-EGFP (AAV1)

Viral prep

#50457-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Promoter

Syn

Tags

EGFP (Cre-dependent)

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only
STAT3-GFP

Plasmid

#110495

Purpose

reporter of STAT3 signaling activity

Depositor

Michael Lewis

Publication

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

4 repeats of STAT3 binding site M67 at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-NLS-HA-Dre

Plasmid

#51272

Purpose

Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

Depositor

Pawel Pelczar

Publication

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Dre

Tags

NLS-HA

Expression

Mammalian

Promoter

CAG

Available since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

Plasmid

#51085

Purpose

Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has service

AAV1 and AAV Retrograde

Insert

GCaMP6f

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Promoter

human Synapsin1

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pmTurquoise2-H2A

Plasmid

#36207

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

H2A (H2AC16 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pLV-Azurite

Plasmid

#36086

Purpose

3rd generation lentiviral plasmid

Depositor

Pantelis Tsoulfas

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pcDNA4-Vps34-Flag

Plasmid

#24398

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Vps34 (PIK3C3 Human)

Tags

3xFLAG

Expression

Mammalian

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pLenti PGK V5-LUC Puro (w543-1)

Plasmid

#19360

Purpose

3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pcDNA3-myc-Skp2

Plasmid

#19947

Depositor

Yue Xiong

Publication

Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Insert

S-phase kinase-associated protein 2 (p45) (SKP2 Human)

Tags

myc

Expression

Mammalian

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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