178,448 results
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Plasmid#174866PurposeExpresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.DepositorInsertT7 RNA polymerase
Tags6XHis-tagExpressionBacterialMutationP266LPromoterlac promoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-jGCaMP8m
Plasmid#162372PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8m
Tags6xHisExpressionMammalianMutationA25G F286YPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP
Plasmid#102796PurposeRetrovirus for delivery of one sgRNA (empty, bbsI)DepositorInsertU6 sgRNA
UseCRISPR and RetroviralExpressionMammalianPromoterU6Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-GFP (AAV1)
Viral prep#37825-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_WKKD
Plasmid#111905PurposeExpress WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybridsDepositorInsertRNASEH1 (RNASEH1 Human)
TagsV5ExpressionMammalianMutationChanged D 210 to N, W 43 to A, K 59 to A, K 60 to…Available sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)
Viral prep#51085-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GroEL/ES-TF
Plasmid#83923PurposeCo-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.DepositorInsertsGro EL/ES
Trigger Factor
ExpressionBacterialPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_nMagHigh1-EGFP-CAAX
Plasmid#67300PurposeBlue light dependent photoswitching systemDepositorInsertnMagHigh1-EGFP-CAAX
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pANAP
Plasmid#48696PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
ExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tet-O-FUW-Neurod1
Plasmid#30129DepositorAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEM-3z/601
Plasmid#26656DepositorInsert
ExpressionBacterialAvailable sinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK7-HA-S6K1-WT
Plasmid#8984DepositorAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-GRAB_DA2h (AAV9)
Viral prep#140554-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA2h (#140554). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA2h plasmid DNA. Synapsin-driven expression of high-affinity GRAB_DA2h dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen2)
Plasmid#73498PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertsdCas9-KRAB
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti SpBsmBI sgRNA Hygro
Plasmid#62205PurposeAn empty sgRNA expression vector for expression of sgRNA for Sp Cas9DepositorHas serviceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianPromoterU6Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-TO-dCas9-3XGFP
Plasmid#64107PurposeExpression of Sp dCas9-3XGFP in mammalian cellsDepositorInsertSp dCas9
UseLentiviralTags3XsfGFPExpressionMammalianPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO5.sgRNA.EFS.tRFP657
Plasmid#57824PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP657
UseCRISPR and LentiviralAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mito-dendra2
Plasmid#55796Purposephoto-activatable mitochondrial marker for expression in mammalian cellsDepositorInsertmito-Dendra2
ExpressionMammalianPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD104 (Peft-3::Cre)
Plasmid#47551PurposeCre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germlineDepositorInsertCre Recombinase
UseCre/LoxExpressionWormPromotereef-1A.1 (eft-3)Available sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSET-mSA
Plasmid#39860DepositorInsertmonomeric streptavidin
Tags6xHis and FLAGExpressionBacterialPromoterT7Available sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by