165,653 results

1. IFN-Beta_pGL3

   Plasmid

   #102597

   Purpose

   IFN-beta promoter driving luciferase. Use in IFN reporter assay.

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   Human IFN-Beta promoter (IFNB1 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. c-Fos

   Plasmid

   #187905

   Purpose

   Expresses c-Fos in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   cFos (FOS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mScarletI3-6DG5-MAPPER

   Plasmid

   #222421

   Purpose

   To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   MAPPER
   

   Use

   Tags

   mScarletI3-6DG5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

   Plasmid

   #183951

   Purpose

   Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9 TadCBEd

   Plasmid

   #193835

   Purpose

   Express TadCBEd (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDd-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p201N Cas9

   Plasmid

   #59175

   Purpose

   Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   nptII
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available Since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR-HOT_tdTomato

   Plasmid

   #138567

   Purpose

   Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   tdTomato
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCRISPRa-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

   Pooled Library

   #84003

   Purpose

   Mouse CRISPRa Pooled Library targeting membrane proteins

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Available Since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pFA6a-6xGLY-3xFLAG-kanMX6

   Plasmid

   #20754

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSyn-Soma-jGCaMP8m (AAV1)

    Viral Prep

    #169257-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-hSyn-Soma-jGCaMP8m (#169257). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8m plasmid DNA. Syn-driven expression of soma-targeted calcium sensor jGCaMP8m. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Promoter

    Tags

    None

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

11. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid

    #50831

    Purpose

    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

    Depositor

    Peter Schultz

    Article

    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Inserts

    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    

    Use

    Baculoviral

    Tags

    His, Myc-6xHis, and WPRE

    Expression

    Insect

    Mutation

    Y39TAG and able to charge various unnatural amino…

    Promoter

    CAG, CMV, and U6 and H1

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Human, Aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-RILP

    Plasmid

    #110498

    Purpose

    Fluorescent RILP

    Depositor

    Terence Dermody, Bernardo Mainou

    Article

    Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

    Insert

    RILP (RILP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. HaloTag-bActinCDS-bActinUTR-MS2V5

    Plasmid

    #102718

    Purpose

    Halo-Actin reporter for detection of both mRNA and protein

    Depositor

    Robert Singer

    Article

    Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    HaloTag-bActinCDS-bActin3'UTR-MS2V5
    

    Use

    Lentiviral

    Tags

    HaloTag and MS2V5

    Expression

    Mutation

    Promoter

    UbC

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG1160 mCherry-FIREtag

    Plasmid

    #205164

    Purpose

    Expresses mCherry -FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. H2B-mCherry

    Plasmid

    #20972

    Purpose

    H2B fused to mCherry for chromatin visualization

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    Histone cluster 1, H2bb (H2BC3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-hM4D(Gi)-mCherry (AAV8)

    Viral Prep

    #50475-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pEVOL-pAzF

    Plasmid

    #31186

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

    Depositor

    Peter Schultz

    Article

    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

    Insert

    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32T, E107N, D158P, I159L, L162Q, D286R

    Promoter

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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