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165,678 results

Showing: 1621 - 1640 of 165678 results

  1. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Use
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  4. pMA7CR_2.0

    Plasmid
    #73950
    Purpose
    pMA7CR_2.0 contains arabinose inducible Ī»/RED Ī²-protein and dam, and aTc inducible CRISPR/Cas9 and recX
    Depositor
    Alex Nielsen
    Article
    Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10
    Inserts
    cas9
    lambda beta
    dam
    recX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pAra and pTet
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    73950-plasmid-map-sequence-id-236989

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  6. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV5)

    Viral Prep
    #20297-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available Since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. pOpen-MMLV_RT (lack H)

    Plasmid
    #165556
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165556-plasmid-map-sequence-id-339910

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  10. pAAV-CaMKIIa-mCherry (AAV5)

    Viral Prep
    #114469-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. pTPGI_dCas9

    Plasmid
    #64279
    Purpose
    encodes yeast-optimized dCas9 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    Yeast-optimized dCas9
    Use
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    D10A & H840A relative to S. pyogenes Cas9
    Promoter
    pTPGI
    Available Since
    May 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    64279-plasmid-map-sequence-id-107786

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  19. pAAV.CAG.GCaMP6s.WPRE.SV40 (AAV9)

    Viral Prep
    #100844-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.CAG.GCaMP6s.WPRE.SV40 (#100844). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6s.WPRE.SV40 plasmid DNA. CAG-driven GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Tags
    None
    Available Since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1621 - 1640 of 165678 results