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Addgene
A versatile plasmid architecture for mammalian synthetic biology (VAMSyB).
Haellman V, Strittmatter T, Bertschi A, Stucheli P, Fussenegger M
Metab Eng. 2021 Jul;66:41-50. doi: 10.1016/j.ymben.2021.04.003. Epub 2021 Apr 18. PubMed Article

Plasmids from Article

ID Plasmid Purpose
169477pVH8-Tier1-PhCMV-NLSTier-1 vector encoding PhCMV-driven nuclear localization signal expression (PhCMV-NLS-pA).
169478pVH9-Tier1-PhCMV-3xNLSTier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).
169479pTS2378-Tier1-PhCMV-KDELTier-1 vector encoding PhCMV-driven ER-retention signal KDEL (PhCMV-KDEL-pA).
169481pTS2412-Tier1-PPGK-2xGSTier-1 vector encoding PPGK-controlled 2x (GGGGS)-linker (PPGK-2xGS-pA).
169482pTS2415-Tier1-PPGK-1xEAAAKTier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).
169484pTS2379-Tier1-PhCMV-PESTTier-1 vector encoding PhCMV-driven PEST tag for destabilization of proteins (PhCMV-PEST-pA).
169485pTS2380-Tier1-PhCMV-TMTier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).
169486pMM585-Tier1-PhCMV-IgkTier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).
169487pTS2381-Tier1-PhCMV-Igk_TMTier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).
169488pTS2409-Tier1-PhCMV-FURINsiteTier-1 vector encoding PhCMV-controlled FURIN protease recognition site (PhCMV-FURINsite-pA).
169489pVH17-Tier1-PhCMV-myrTier-1 vector encoding PhCMV-driven myristoylation (myr) signal peptide expression (PhCMV-myr-pA).
169490pTS1051-Tier1-PhCMV-4xCox8Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)
169491pTS1018-Tier1-PhCMV-p2ATier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).
169492pTS1021-Tier1-PhCMV-PuroRTier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)
169493pTS2401-Tier1-PhCMV-HygroRTier-1 vector encoding PhCMV-driven hygromycin resistance gene HygroR (PhCMV-HygroR-pA).
169494pTS2402-Tier1-PhCMV-BlastRTier-1 vector encoding PhCMV-driven blasticidin resistance gene BlastR (PhCMV-BlastR-pA).
169495pTS2403-Tier1-PhCMV-ZeoRTier-1 vector encoding PhCMV-driven zeocin resistance gene ZeoR (PhCMV-ZeoR-pA).
169496pTS2404-Tier1-PhCMV-NeoRTier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).
169497pTS1023-Tier1-PhCMV-p2A-PuroRTier-1 vector encoding PhCMV-driven p2A sequence fused to a marker for puromycine selection (PhCMV-p2A-PuroR-pA).
169498pTS1047-Tier1-PhCMV-YPet-p2A-PuroRTier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).
169499pTS1024-Tier1-PRPBSA-YPet-p2A-PuroRTier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)
169500pTS2405-Tier1-PhCMV-TEVTier-1 vector encoding PhCMV-driven tabacco etch virus protease TEV (PhCMV-TEV-pA).
169501pTS2406-Tier1-PhCMV-NTEVTier-1 vector encoding PhCMV-driven N-terminal fragment of a split tabacco etch virus protease splitTEV (PhCMV-N-TEV-pA).
169503pTS2408-Tier1-PhCMV-TEVsiteTier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).
169505pMM545-Tier1-PhCMV-CitrineTier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).
169506pFOX8-Tier1-PhCMV-YPetTier-1 vector encoding PhCMV-driven YPet expression (PhCMV-YPet-pA).
169508pFOX11-Tier1-PhCMV-CloverTier-1 vector encoding PhCMV-driven gren fluorescent protein clover (PhCMV-clover-pA).
169509pFOX12-Tier1-PhCMV-eGFPTier-1 vector encoding PhCMV-driven green fluorescent protein eGFP (PhCMV-eGFP-pA).
169510pFOX13-Tier1-PhCMV-tGFPTier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).
169511pFOX14-Tier1-PhCMV-mTFP1Tier-1 vector encoding PhCMV-driven green fluorescent protein mTFP1 (PhCMV-mTFP1-pA).
169512pFOX15-Tier1-PhCMV-mTurquoise2Tier-1 vector encoding PhCMV-driven green fluorescent protein mTurquoise2 (PhCMV-mTurquoise2-pA).
169515pFOX6-Tier1-PhCMV-TdTomatoTier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).
169517pTS2384-Tier1-PhCMV-medFTTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT (PhCMV-medFT-pA).
169518pTS2385-Tier1-PhCMV-fastFTTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).
169519pTS2386-Tier1-PhCMV-GCaMP6sTier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).
169520pVH231-Tier1-PmPGK1-mTagBFP2Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).
169521pVH230-2-Tier1-PmPGK1-YPetTier-1 vector encoding PmPGK1-driven YPet expression (PmPGK1-YPet-pA).
169522pVH233-Tier1-PmPGK1-eYFPTier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).
169523pVH232-Tier1-PmPGK1-mRuby2Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).
169524pTS2387-Tier1-PhCMV-p2a_mTagBFP2Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).
169525pTS2388-Tier1-PhCMV-p2a_YpetTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).
169526pTS2389-Tier1-PhCMV-p2a_mRubyTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).
169529pTS2392-Tier1-PhCMV-p2a_medFTTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).
169530pTS2393-Tier1-PhCMV-p2a_fastFTTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).
169531pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).
169532pTS1026-Tier1-PhCMV-3xNLS-iRFP670Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).
169533pTS2395-Tier1-PhCMV-Igk_mRuby2_KDELTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).
169534pTS2396-Tier1-PhCMV-4xCOX8_YpetTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).
169535pTS2410-Tier1-PhCMV-Myr_mTagBFP2Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).
169537pTS1046-Tier1-PhCMV-4xCox8-mRuby2Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)
169543pVH608-Tier1-PmPGK1-FlucTier-1 vector encoding PmPGK1-driven Fluc (PmPGK1-Fluc-pA).
169544pVH41-Tier1-PhCMV-GLucTier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)
169545pTS1011-Tier1-PhCMV-NlucTier-1 vector encoding PhCMV-driven NLuc (PhCMV-NLuc-pA).
169548pTS2303-Tier1-CoTC-PPGK-NLuc_TBTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
169549pTS2304-Tier1-Tactb-PPGK-NLuc_TBTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
169550pTS2307-Tier1-2xcHS4-PPGK-NLuc_TBTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
169551pTS2308-Tier1-pGL3-PPGK-NLuc_TBTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
169552pTS2311-Tier1-spacer-PPGK-NLuc_TBTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
169557pVH63-Tier1-PMLP-Citrine_p2a_SEAPTier-1 vector encoding PMLP-driven Citrine-p2A-SEAP expression (PMLP-Citrine-p2A-SEAP-pA).
169558pVH64-Tier1-PSV40-Citrine_p2a_SEAPTier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).
169559pVH65-Tier1-PTK-Citrine_p2a_SEAPTier-1 vector encoding PTK-driven Citrine-p2A-SEAP expression (PTK-Citrine-p2A-SEAP-pA).
169560pVH72-Tier1-PhCMV-SEAPTier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).
169561pVH21-Tier1-PhEF1aTier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).
169562pTS2364-Tier1-IRESTier-1 vector carrying an internal ribosome entry site (IRES-pA)
169563pVH227-Tier1-OTetO7-PCMVmin2-SEAPTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).
169564pVH228-Tier1-OVanO2-PCMVmin2-SEAPTier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).
169565pVH229-Tier1-OTtgO2-PCMVmin2-SEAPTier-1 vector encoding PTtgO2-driven SEAP expression (OTtgO2-PCMVmin-2-SEAP-pA).
169566pTS2365-Tier1-PhCMV-TetRTier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).
169567pTS2366-Tier1-PhCMV_F-typeTier-1 vector encoding PhCMV-driven F-type transactivator domain (PhCMV-F-type-pA).
169568pTS2367-Tier1-PhCMV_VP16Tier-1 vector encoding PhCMV-driven VP16 transactivator domain (PhCMV-VP16-pA).
169569pTS2368-Tier1-PhCMV_VP64Tier-1 vector encoding PhCMV-driven VP64 transactivator domain (PhCMV-VP64-pA).
169570pVH15-Tier1-PhCMV-VPRTier-1 vector encoding PhCMV-driven VP64-P65-Rta expression (PhCMV-VPR-pA)
169571pTS2369-Tier1-PhCMV_KRABTier-1 vector encoding PhCMV-driven KRAB transsilencer domain (PhCMV-KRAB-pA).
169572pVH19-Tier1-PhCMV-p300coreTier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)
169573pTS2370-Tier1-PhCMV-TetR_F-typeTier-1 vector encoding PhCMV-driven tetracycline-dependent F-type-based transactiavtor (PhCMV-TetR-F-type-pA).
169574pVH234-Tier1-PmPGK1-tTATier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).
169575pAB303-Tier1-PhCMV-TetR_VPRTier-1 vector encoding PhCMV-driven tetracycline-dependent VPR-based transactiavtor (PhCMV-TetR-VPR-pA).
169577pAB300-Tier1-PhCMV-rTetRTier-1 vector encoding PhCMV-driven tetracycline-activated rTetR (PhCMV-rTetR-VPR-pA).
169578pVH235-Tier1-PmPGK1-rtTATier-1 vector encoding PmPGK1-driven rtTA expression (PmPGK1-rtTA-pA).
169579pAB301-Tier1-PhCMV-rTetR_VPRTier-1 vector encoding PhCMV-driven tetracycline-activated VPR-based transactiavtor (PhCMV-rTetR-VPR-pA).
169581pTS2373-Tier1-PhCMV-Gal4Tier-1 vector encoding PhCMV-driven Gal4 DNA binding domain (PhCMV-Gal4-pA).
169582pTS2374-Tier1-PhCMV-Gal4_VP16Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).
169584pVH237-2-Tier1-PmPGK1-TtgATier-1 vector encoding PmPGK1-driven TtgA expression (PmPGK1-TtgA-pA).
169585pVH254-Tier1-OTetO7-Pmin-SEAPTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-Pmin-SEAP-pA).
169586pTS1017-Tier1-OTetO7-PCMVmin-SEAPTier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).
169587pVH255-Tier1-OTetO7-PCMVmin1-SEAPTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-1-SEAP-pA).
169588pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAPTier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).
169589pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSVTier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).
169590pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).
169591pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).
169592pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).
169594pVH607-Tier1-PCREm4-NlucTier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).
169595pVH321-1-Tier1-PhCMV-dCas9-3xNLSTier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).
169596pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLSTier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).
169597pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).
169599pVH1001-Tier2(ColE1 ori)Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and ColE1 ori).
169600pVH1002-Tier2(pUC ori)Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).
169602pVH613Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).
169603pVH614Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).
169604pVH615Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).
169605pVH616Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).
169606pVH617Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).
169607pVH663Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).
169608pVH664Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).
169610pVH666Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).
169611pTS1031Tier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).
169612pTS1032Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).
169613pTS1033Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).
169614pTS1035Tier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).
169615pTS1036Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).
169616pTS1037Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).
169617pTS1039Tier-2 vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::A3-pA).
169618pTS1040Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
169619pTS1041Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
169620pTS1042Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
169621pTS1043Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .
169622pTS2312Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
169624pTS2317Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
169625pTS2320Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
169626pTS2321Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
169627pTS2322Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
169628pTS2326Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
169629pTS2329Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
169630pgTS40pX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.
169632pTS2163Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)
169633pTS395_PhCMV-SB100Expression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)
169634pTS1107_Tier3(SB)Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase (5'ITR-A1-pA::A2-pA::A3-pA-3'ITR)
169635pTS2337_Tier3(SB)-TagBFP2_PuroTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)
169637pTS2339_Tier3(SB)-Ruby_PuroTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)
169638pTS2340_Tier3(SB)-iRFP_PuroTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
169639pTS2341_Tier3(SB)-PuroTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)
169641pTS2343_Tier3(SB)-YPet_BlastTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)
169642pTS2344_Tier3(SB)-mRuby2_BlastTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-BlastR-pA-3'ITR)
169648pTS2351_Tier3(SB)-ZeoTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)
169649pTS1019_Tier3(PB)Tier-3 vector for stable integration of up to three cassettes via piggyBac transposase (ITR-A1::A2::A3-ITR)
169652pTS2353_Tier3(PB)-YPet_PuroRTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
169653pTS2354_Tier3(PB)-Ruby_PuroRTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)
169654pTS2355_Tier3(PB)-iRFP_PuroRTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
169655pTS2356_Tier3(PB)-PuroRTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)
169656pTS2357_Tier3(PB)-TagBFP_BlastRTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mTagBFP2 marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-BlastR-pA-3'ITR)
169657pTS2361_Tier3(SB)-rtTA-YPet_PuroRTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
169658pTS2362_Tier3(SB)-rtTA-PuroRTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
169659pTS2363_Tier3(PB)-rtTA-YPet_PuroRTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
169661pTS1106_Tier3(Lenti)Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction (PCMV-5'LTR-Psi-gag-env-A1::A2::A3-WPRE-3'LTR)
169663pTS1108_Tier3(Lenti_inverse)Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction in inverted direction with polyA (PCMV-5'LTR-Psi-gag-env-A3-pA::A2-pA::A1-pA-WPRE-3'LTR)
169664pTS2333_Tier3(Lenti_inverse)-YPet_PuroTier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)
169665pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_PuroTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)
169667pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

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