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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,957 results for: pET

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Showing: 1721 - 1740 of 9957 results

  2. pET-DEST42-dre4

    Plasmid
    #170440
    Purpose
    Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.
    Depositor
    Joan Conaway
    Publication
    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e
    Insert
    dre4 (dre4 Fly)
    Tags
    6xHis and V5
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-20
    Promoter
    T7
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    170440-plasmid-map-sequence-id-338015

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  20. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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Showing: 1721 - 1740 of 9957 results