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AR-V7-pcw107-V5

Plasmid

#64636

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

V5

Mutation

splice isoform *

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pCW57-mCherry-2A-BRD4 Iso C

Plasmid

#137721

Purpose

Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoform

Depositor

Scott Floyd

Article

Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

Insert

BRD4 short isoform (BRD4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pet-15b-microID

Plasmid

#172881

Purpose

expresses microID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDA027

Plasmid

#131385

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A, D702Y

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Tp-SceI-pheS

Plasmid

#111608

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET16better_H10_HRV3C_chitinase

Plasmid

#175091

Purpose

Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

Depositor

Jay C Dunlap

Article

Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

Insert

chitinase

Tags

10X His, HRV-3C

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDA093

Plasmid

#131390

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pZ005 (AacC2c1 human codon optimized protein)

Plasmid

#70171

Purpose

Expresses AacC2c1 human codon optimized protein

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

AacC2c1 protein

Tags

NLS

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTOX11

Plasmid

#127456

Purpose

Encodes MqsR toxin. Gent-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

mqsR toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pUCBB-oriT

Plasmid

#32556

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

oriT

Use

Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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FRT_htr2c(c)-GFP

Plasmid

#79677

Purpose

serotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFP

Depositor

Stefan Stamm

Article

Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

Insert

serotonin receptor 2C (HTR2C Human)

Tags

EGFP in Frame

Expression

Mammalian

Mutation

splice site of exon Vb in consensus

Promoter

CMV

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only
Anti-HCN1 [N70/28R] (Recombinant Antibody)

Antibody

#180092-rAb

Purpose

Anti-HCN1 (Rat) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunohistochemistry and Western Blot

Reactivity

Human, Mouse, and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available Since

March 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits
PITPbeta-GFP

Plasmid

#58274

Purpose

mammalian expression of GFP-PITP beta

Depositor

Shamshad Cockcroft

Article

Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

Insert

PITP beta (PITPNB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
GST-U1C

Plasmid

#1840

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

U1C (SNRPC Human)

Tags

GST

Expression

Bacterial

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-cj-E sgRNA

Plasmid

#169915

Purpose

Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTR47m4-GFP

Plasmid

#102436

Purpose

Formaldehyde biosensor plasmid with sGFP reporter and modified frm promoter

Depositor

Gregory Stephanopoulos

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

Formaldehyde repressor protein (frmR)
Superfolder GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-mKate2 no-pA

Plasmid

#80828

Purpose

P2A followed by mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-mKate2 no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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p4580 AU-IRF-3a

Plasmid

#8660

Depositor

Peter Howley

Article

Karpova et al Mol Cell Biol 2001 Jul;21(13):4169-76.

Insert

IRF-3a (IRF3 Human)

Tags

AU

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
mARGAna (cassette 1, transient)

Plasmid

#197588

Purpose

Second-generation mammalian acoustic reporter gene derived from Anabaena

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

mARGAna (cassette 1)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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