pMslc
(Plasmid
#32095)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32095 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMONO-blasti
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Backbone manufacturerInvivoGen
- Backbone size w/o insert (bp) 3435
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Blasticidin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSLC35A1
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Alt namesolute carrier family 35 (CMP-sialic acid transporter), member A1
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Alt nameCMP-sialic acid transporter
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SpeciesH. sapiens (human)
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Insert Size (bp)1839
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Entrez GeneSLC35A1 (a.k.a. CDG2F, CMPST, CST, hCST)
- Promoter FerH
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer TGAGCGGAGCTAATTCTCG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please consult laboratory website, http://home.ccr.cancer.gov/Lco/plasmids.asp, for more information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMslc was a gift from Christopher Buck (Addgene plasmid # 32095 ; http://n2t.net/addgene:32095 ; RRID:Addgene_32095) -
For your References section:
Glycosaminoglycans and sialylated glycans sequentially facilitate merkel cell polyomavirus infectious entry. Schowalter RM, Pastrana DV, Buck CB. PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28. 10.1371/journal.ppat.1002161 PubMed 21829355
Map uploaded by the depositor.
