We narrowed to 3,776 results for: golden gate

1. pET22b-EK-cleavableELP

   Plasmid

   #219404

   Purpose

   Enterokinase-responsive elastin-like polypeptide (ELP)

   Depositor

   Danielle Mai

   Article

   Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

   Insert

   EK-cleavableELP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-Thr-cleavableELP

   Plasmid

   #219405

   Purpose

   Thrombin-responsive elastin-like polypeptide (ELP)

   Depositor

   Danielle Mai

   Article

   Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

   Insert

   Thr-cleavableELP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBTK1059

   Plasmid

   #209549

   Purpose

   BTK Type 5 homology arm for Golden Gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Lariviere et al bioRxiv 2023.09.19.558440

   Insert

   Downstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. GGDestX2-Amp

   Plasmid

   #157654

   Purpose

   Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ131C-RZ-Lb

   Plasmid

   #134347

   Purpose

   Golden Gate entry vector; empty vector to clone the 1st LbCas12a crRNA under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL12022

   Plasmid

   #117510

   Purpose

   AtAG promoter Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:GGAG:pAG:AATG:BsaI
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtAG

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJet1.2 'rncS

   Plasmid

   #117129

   Purpose

   smc gene upstream homology region

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   'rncS
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGGE003

   Plasmid

   #48854

   Purpose

   Provides the At1g04880 terminator as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   At1g04880 terminator
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGGC014

   Plasmid

   #48827

   Purpose

   Provides GFP as GreenGate coding sequence module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   GFP
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   A206K

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGGC024

    Plasmid

    #48829

    Purpose

    Provides BFP as GreenGate coding sequence module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    BFP
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. EC71174

    Plasmid

    #154067

    Purpose

    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Inserts

    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    

    Use

    Cre/Lox and Synthetic Biology; Golden gate

    Tags

    Expression

    Bacterial and Plant

    Mutation

    The Arabidopsis thaliana U5 small nuclear ribonuc…

    Promoter

    35S promoter, Hordeum vulgare HSP 17 promoter use…

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-hEF1A-MG-U6-sgRNA

    Plasmid

    #175505

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a, U6

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Platinum Gate TALEN Kit

    Plasmid Kit

    #1000000043

    Purpose

    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.

    Depositor

    Takashi Yamamoto

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pLV GG hUbC-dsRED

    Plasmid

    #84034

    Purpose

    Contains Golden Gate cassette to express up to four gRNAs and dsRED

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCR8/GW:U6

    Plasmid

    #85981

    Purpose

    Golden Gate Level 0 cassette encoding the U6 promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    T. pseudonana U6 promoter
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJet1.2 ermB cassette

    Plasmid

    #117121

    Purpose

    Erythromycin resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Erythromycin resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJPC210

    Plasmid

    #234532

    Purpose

    Int13 Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJPC063

    Plasmid

    #234533

    Purpose

    TRV2 expressing Int13

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    Integrase 13 recombinase
    

    Use

    Recombinase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJPC062

    Plasmid

    #234534

    Purpose

    TRV2 expressing CinH

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    CinH Recombinase
    

    Use

    Recombinase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJPC430

    Plasmid

    #234529

    Purpose

    CinH Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge