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Plasmid#172861PurposeExpresses pFAST in mammalian cellsDepositorInsertpFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFast-hRad1
Plasmid#222471PurposeExpresses hRad1 in insect cellsDepositorInserthRad1 (RAD1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFast-hHus1
Plasmid#222469PurposeExpresses hHus1 in insect cellsDepositorInserthHus1 (HUS1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFast-FT-N1
Plasmid#31910DepositorInsertFast-FT
UseTagsExpressionMammalianMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M2
Plasmid#135583PurposeHigh eukaryotic protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_human-IL-18
Plasmid#214338PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam3_N_His8_eGFP_3C
Plasmid#178807PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTagsHis8, eGFP, 3CExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_HCK
Plasmid#224245PurposeExpression of human HCK Kinase in insect cells (SF9 cells)DepositorInserthck (HCK Human)
UseTags6xHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam4_N_Flag_His8_eGFP_TEV
Plasmid#178808PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTagsFlag, His8, eGFP, TEVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam7_N_Flag_TEV
Plasmid#178817PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTagsFlag, TEVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam2_N_His8_eGFP_TEV
Plasmid#178806PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteinsDepositorTypeEmpty backboneUseTagsHis8, eGFP, TEVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam8_N_Flag_3C
Plasmid#178818PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTagsFlag, 3CExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam5_N_Flag_His8_eGFP_3C
Plasmid#178816PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTagsFlag, His8, eGFP, 3CExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam6_C_3C_His8_Stop
Plasmid#178810PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTags3C, His8, Stop codonExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_LYN
Plasmid#224244PurposeExpression of human LYN Kinase in insect cells (SF9 cells)DepositorInsertlyn (LYN Human)
UseTags6xHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_SYK
Plasmid#224246PurposeExpression of human SYK Kinase in insect cells (SF9 cells)DepositorInsertsyk (SYK Human)
UseTags6xHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-melHis10TEV
Plasmid#159428Purposeempty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sitesDepositorTypeEmpty backboneUseTagsmelittin signal sequence (removed in vivo by sign…ExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-M2
Plasmid#135584PurposeHigh eukaryotic dual protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10FLAG
Plasmid#157736Purposeempty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsHis10 + 3xFLAG, enterokinase-cleavableExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only