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Showing: 41 - 60 of 95 results

  1. EasyCloneYALI- Integrative Vector Set

    Plasmid Kit
    #1000000140
    Purpose
    Integrative plasmids for overexpression of heterologous genes in Yarrowia lipolytica.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  2. CIDAR MoClo Extension, Volume I

    Plasmid Kit
    #1000000161
    Purpose
    Plasmids to efficiently build a wide variety of gene expression constructs. Can be used as a stand-alone toolkit or with the CIDAR MoClo Parts Kit (Addgene #1000000059).
    Depositor
    Richard Murray
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  3. Chromoprotein kit 2.0

    Plasmid Kit
    #1000000198
    Purpose
    Expression of visible chromoproteins in E. coli, including two new chromoprotein genes and improved stability.
    Depositor
    Anthony Forster
    Application
    For the Classroom, Gene Expression and Labeling
    Vector Type
    Bacterial Expression
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only

  4. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit
    #1000000240
    Purpose
    Collection of plasmids to optimize secretion and cell surface display of proteins of interest in yeast.
    Depositor
    Paul Young
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  5. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Assembly of transcription units for protein secretion in the yeasts Saccharomyces cerevisiae and Pichia pastoris.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  6. Cultivarium POSSUM Toolkit

    Plasmid Kit
    #1000000234
    Purpose
    Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model bacteria.
    Depositor
    Nili Ostrov
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (Loop)
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only

  7. GreenGate 2.0 Toolkit

    Plasmid Kit
    #1000000241
    Purpose
    Compatible with GreenGate Cloning System (#1000000036). Includes modules for CRISPR/Cas9 genome editing, dexamethasone-inducible expression, and fluorescent plasma membrane and nuclear markers.
    Depositor
    Alexis Maizel
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Plant Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (GreenGate)
    Available Since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. MultiSite Gateway plasmid toolKit

    Plasmid Kit
    #1000000050
    Purpose
    Plasmids for constructing fully-customizable multi-part fusion proteins.
    Depositor
    Tomas Santalucia
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression, Eukaryotic Expression, Mammalian Expression
    Cloning Type
    MultiSite Gateway
    Available Since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit
    #1000000138
    Purpose
    This toolkit allows generation of transcriptional units using GoldenBraid for integration in the S. cerevisiae genome at loci, YPRCΔ15 and YORWΔ22, to support high and stable transgene expression.
    Depositor
    Elena Caro Bernat
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (GoldenBraid)
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. FNL Combinatorial Cloning Platform

    Plasmid Kit
    #1000000211
    Purpose
    A Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.
    Depositor
    Dominic Esposito
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression
    Cloning Type
    MultiSite Gateway
    Available Since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  11. 4G-Cloning Plasmid Kit

    Plasmid Kit
    #1000000252
    Purpose
    Use 4G (Golden-Gate-guided Gibson) cloning for rapid assembly of single- or multi-gene expression vectors for multi-subunit protein complexes in various expression hosts.
    Depositor
    Stephan Gruber
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only

  12. GRASP Cloning Kit

    Plasmid Kit
    #1000000282
    Purpose
    A MoClo-compatible collection of 42 plasmids that enables the high-efficiency assembly of RNA-binding protein subunits.
    Depositor
    Ian Small
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression, Mammalian Expression, Plant Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    May 20, 2026
    Availability
    Academic Institutions and Nonprofits only

  13. Yeast GPCR-sensor Toolkit

    Plasmid Kit
    #1000000157
    Purpose
    This toolkit includes plasmids for constructing highly-tunable GPCR-based biosensors in yeast.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only

  14. Fragmid

    Plasmid Kit
    #1000000237
    Purpose
    Toolkit for rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.
    Depositor
    John Doench
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    AAV, Insect Expression, Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only

  15. Komagataeibacter Tool Kit (KTK)

    Plasmid Kit
    #1000000216
    Purpose
    A collection of standardized parts used for hierarchical assembly of single and multi-gene constructs for Komagataeibacter species.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only

  16. EGFP/mCherry FLIM/FRET Kit

    Plasmid Kit
    #1000000148
    Purpose
    Plasmids for analysis of protein-protein interactions in planta via Förster Resonance Energy Transfer (FRET) using EGFP and mCherry.
    Depositor
    Christopher Grefen
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Plant Expression
    Cloning Type
    Gateway
    Available Since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only

  17. Start-Stop Assembly Toolkit

    Plasmid Kit
    #1000000152
    Purpose
    Modular, scarless DNA assembly method suitable for combinatorial assembly and ideal for metabolic engineering.
    Depositor
    John Heap
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only

  18. Mobius Assembly Vector Toolkit

    Plasmid Kit
    #1000000134
    Purpose
    Mobius Assembly is a Golden Gate-based cloning framework which renders both high cloning capacity and vector toolkit simplicity.
    Depositor
    Naomi Nakayama
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    March 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. UbiGate Components (set1)

    Plasmid Kit
    #1000000144
    Purpose
    UbiGate Components (set 1) enables the reconstitution of the ubiquitination cascades of different organisms, including A. thaliana, in E. coli by 'Golden Gate' cloning.
    Depositor
    Marco Trujillo
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  20. FusX TALEN assembly system

    Plasmid Kit
    #1000000063
    Purpose
    Streamlined Golden Gate TALE assembly of 15–16 RVD TALEN inserts in one step that is backwards compatible with popular TALE backbones for expression in multiple systems; requires additional plasmids for use.
    Depositor
    Stephen Ekker
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Zebrafish Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 60 of 95 results