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Synthetic Biology plasmids for cloning and genomic tools.

FastCloning is a fast and easy technique that can replace restriction cloning. Learn about this great plasmid cloning technique here.

Learn about the Fibrodysplasia ossificans progressiva (FOP) and how you can help Addgene fight this rare disease at the Cambridge Superhero 5k.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Advice for scientists choosing a lab, including interviews with Principal Investigators and important factors to consider in your decision.

Quickly isolate mammalian cells clones using the agarose cloning method.

Use CcdB to enhance the efficiency of your next plasmid cloning experiment.

Advice for things you can do to develop transferable skills while you are also learning to be an excellent scientist.

Learn about the many resources you can use for career development as a graduate student or post doc.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Angela Depace's lab recently described how Individual development plans (IDP's) help advance their research. Read on to learn more about IDPs.

Read about Gladys Kaba, the 3rd recipient of the Addgene/Seedinglabs Plasmid Grant, who will be using plasmids as positive controls in cervical cancer screens.

Looking for a way to test a lot of different parts in your cloning, or maybe interrogate some complex genetic circuits? MoClo just might be the approach for you!

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

TOPO cloning is a restriction enzyme and ligase free cloning method. Learn how TOPO works in this post.