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SynBio_Icon_Cloning.png Synthetic Biology: Cloning and Genomic Tools


Browse Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems.

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Plasmids for Cloning and Genomic Tools

Search the table by keyword or sort by the table headings. Click on the publication link to view all plamsids available from the article. Click on the PI link to view all plasmids available from this depositor's lab.

Plasmid Purpose Gene/Insert Vector Type PI Publication
pJRL2 PHO5pr VYFP (EB#1547)PHO5 promoter (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 PHO5(UASm1)pr VYFP (EB#1549)PHO5 promoter UASm1 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)PHO5 promoter TATA-C2 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)PHO5 promoter TATA-A1T6 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 GAL1pr VYFP (EB#1571)GAL1 promoter (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)GAL1 promoter TATA-A1T6 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)GAL1 promoter TATA-A1T6 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)GAL1 promoter TATA-C2 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)GAL1 promoter TATA-C2 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pPro29b(+)Bacterial Expression, Synthetic Biology Keasling Keasling Lab (unpublished)
pMLLKA-J72016N terminal 6X His tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72017N terminal avi tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72018N terminal E tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72019N terminal Flag tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72020N terminal HA tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72021N terminal HSV tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72022N terminal myc tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72023N terminal S tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72024N terminal Strep tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72025N terminal T7 tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72026N terminal V5 tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72027N terminal VSV tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72028C terminal 6XHIS tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72029C terminal avi tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72030C terminal E tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-K112603C terminal Flag tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-K112507C terminal HA tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72031C terminal HSV tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72032C terminal S tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72033C terminal Strep tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72034C terminal T7 tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72035C terminal V5 tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72036C terminal VSV tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72037Med7 aa102-205 for N-terminal taggingSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72038Med21 aa1-132 for N-terminal tagging (Saccharomyces cerevisiae)Synthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72039Med7 aa102-205 for C-terminal tagging (Saccharomyces cerevisiae)Synthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72040Med21 aa1-132 for C-terminal tagging (Saccharomyces cerevisiae)Synthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLAC-J72041Tev Protease Cleavage siteSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pUCBB-eGFPeGFPBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pBBRBB-eGFPeGFPBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pCDFBB-eGFPegfpSynthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pACBB-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-pT7-eGFPeGFPBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-pBAD-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-ptet-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pACBB-TetT-LVATetRBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-oriToriTBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-ntH6-eGFPeGFPBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-ctH6-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-lacZaLacZ-alphaBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pBbE8a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE8c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE8k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA8a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA8c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA8k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS8a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS8c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS8k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE5a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE5c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE5k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA5a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA5c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA5k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS5a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS5c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS5k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE6a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE6c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE6k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA6a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA6c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA6k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS6a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS6c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS6k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE4a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE4c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE4k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA4a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA4c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA4k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS4a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS4c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS4k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE3a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE3c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE3k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA3a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA3c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA3k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS3a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS3c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS3k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE7a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE7c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE7k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA7a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA7c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA7k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS7c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS7k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE2a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE2c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE2k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA2a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA2c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA2k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS2a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS2c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS2k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE1a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE1c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE1k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA1a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA1c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA1k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS1a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS1c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS1k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB1a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB1c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB1k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB2a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB2c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB2k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB3a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB3c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB3k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB4a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB4c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB4k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB5a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB5c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB5k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB6a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB6c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB6k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB7a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB7c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB7k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB8a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB8c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB8k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE0c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE0k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB0c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS0a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS0k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB0k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB0a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE0a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA0c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA0k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA0a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pRibo BsaHindBacterial Expression, Synthetic Biology ; Mycobacteria replicating vector Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pRibo EcoHindBacterial Expression, Synthetic Biology ; Mycobacteria replicating vector Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pRiboS BsaHindBacterial Expression, Synthetic Biology ; Mycobacteria replicating vector Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pRiboS EcoHindBacterial Expression, Synthetic Biology Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pST5552Mycobacteria replicating vector expressing eGFP.eGFPBacterial Expression, Synthetic Biology ; Mycobacteria replicating vector Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pST5832lacZBacterial Expression, Synthetic Biology ; Mycobacteria replicating vector Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
BBa_J72110-BBa_J72094AraC-pBad + coiBacterial Expression, Synthetic Biology Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72110-BBa_J72097RhaRS-pRhaB + CoiBacterial Expression, Synthetic Biology Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72110-BBa_J72103AraC-pBad + coi + cin + repL + pacABacterial Expression, Synthetic Biology ; Phagemid Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72114-BBa_J72100lacZBacterial Expression, Synthetic Biology ; Phagemid Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72214-BBa_J72090VioABCDEBacterial Expression, Synthetic Biology ; phagemid Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72114-BBa_J72104RhaRS + pRhaB + LuxCDABE, VioABCE, LacZBacterial Expression, Luciferase, Synthetic Biology ; Phagemid Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72113-BBa_J72152Phagemid + GFPBacterial Expression, Synthetic Biology Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72113-BBa_J72103AraC-pBad + coi + cin + repL + pacABacterial Expression, Synthetic Biology Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
pNH001TAP-tag (Caenorhabditis elegans)Worm Expression Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH002N-TAP TAG::[(G4S)3]::mTFP1 (Caenorhabditis elegans)Worm Expression Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH009N-TAP-tag::mTFP1 (Caenorhabditis elegans)Worm Expression Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH013mTFP1 (Caenorhabditis elegans)Synthetic Biology Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH026mTFP1::linker::TAP TAG (Caenorhabditis elegans) Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH030mTFP1::linker::2xNLS (Caenorhabditis elegans)Worm Expression Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH034RT-cassette (Synthetic) Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH039CFP Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH040GFP Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH041YFP Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH042mCherry Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH043mTFP1 Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH050CFP-RT-CFP Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH051GFP-RT-GFP Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH052YFP-RT-YFP Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH053mCherry-RT-mCherry Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH054mTFP1-RT-mTFP1 Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH058TAP Tag::mTFP1 Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH066N-TAP Tag-RT-::mTFP1 (Caenorhabditis elegans) Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH078mTFP1 (Caenorhabditis elegans)Worm Expression Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH082mTFP1 (Caenorhabditis elegans)Worm Expression Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH090mTFP1::[(G4S)3]::C-TAP Tag (Caenorhabditis elegans) Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pNH094mTFP1-RT-::[(G4S)3]::C-TAP Tag (Caenorhabditis elegans) Dolphin A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. BMC Biotechnol. 2013 Jan 3;13(1):1.
pCsy4csy4 geneBacterial Expression, Synthetic Biology Arkin RNA processing enables predictable programming of gene expression. Nat Biotechnol. 2012 Oct;30(10):1002-6. doi: 10.1038/nbt.2355. Epub 2012 Sep 16.
BB-­NIC-­II-­HisN-­TET12TET12 (Synthetic)Bacterial Expression, Synthetic Biology Jerala Design of a single-chain polypeptide tetrahedron assembled from coiled-coil segments. Nat Chem Biol. 2013 Apr 28. doi: 10.1038/nchembio.1248.
pBADmod1-linker2-gamSgamS (Other)Bacterial Expression, Synthetic Biology Murray Murray Lab gamS and ClpX plasmids (unpublished)
pE-FLPflpBacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-CH (CamR, HK022)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-CT (CamR, P21)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KC (KanR, PhiC31)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KH (KanR, HK022)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KL (KanR, Lambda)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KO (KanR, 186)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KP (KanR, Phi80)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KT (KanR, P21)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-TH (TetR, HK022)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-TT (TetR, P21)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pGRReporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinoseBacterial Expression, Synthetic Biology Voigt Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-ACC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-GGG/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-CCC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-UCU/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-/CGA-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-/UAA-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-/GCCACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-ACC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-GGG/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-CCC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-UCU/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-/CGA-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-/UAA-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-/GCCACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pBS-67SmaChlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic regionEcoRI 5.8 kB (Other)Synthetic Biology Melis Marker-free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii. Plant Biotechnol J. 2013 Sep;11(7):818-28. doi: 10.1111/pbi.12073. Epub 2013 May 7.
pJ207 Delta-cpc-KanRDeletes the cpc operon in Synechocystis and confers kanamycin resistanceReplacing the cpc operon with kanamycin resistance (Synthetic)Synthetic Biology Melis Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size. Biochim Biophys Acta. 2014 Oct;1837(10):1653-64. doi: 10.1016/j.bbabio.2014.07.009. Epub 2014 Jul 18.
pBullet-cyt-ndestination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant geneSynthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-cyt-cdestination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant geneSynthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-er-ndestination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant gene Synthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-er-cdestination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant geneSynthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-cg-ndestination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (N-ter-EYFP) of plant geneSynthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-cg-cdestination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant geneSynthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-tgn-ndestination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant geneSynthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-tgn-cdestination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant gene Synthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-end-ndestination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (N-ter-EYFP) of plant gene Synthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-end-cdestination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (C-ter-EYFP) of plant geneSynthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-vac-ndestination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (N-ter-EYFP) of plant geneSynthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-vac-cdestination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene Synthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-pm-ndestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant gene Synthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pBullet-pm-cdestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant gene Synthetic Biology Heazlewood The Plant Glycosyltransferase Clone Collection for Functional Genomics. Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
pCS1Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.KanR (Other)Bacterial Expression Bennett Stable maintenance of multiple plasmids in E. coli using a single selective marker. ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
pCS5Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.KanR (Other)Bacterial Expression Bennett Stable maintenance of multiple plasmids in E. coli using a single selective marker. ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
pCS4Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.KanR (Other)Bacterial Expression Bennett Stable maintenance of multiple plasmids in E. coli using a single selective marker. ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
pCS6Produces T7 RNA Polymerase.T7 RNA Polymerase (Other)Bacterial Expression Bennett Stable maintenance of multiple plasmids in E. coli using a single selective marker. ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
pSEVAT1Z1AGeneGuard vector cassette: thyA, R6K COR, Zeta toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD1Z1AGeneGuard vector cassette: dapA, R6K COR, Zeta toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAT1K1AGeneGuard vector cassette: thyA, R6K COR, Kid toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD1K1AGeneGuard vector cassette: dapA, R6K COR, Kid toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAT7Z1AGeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD7Z1AGeneGuard vector cassette: dapA, ColE2 COR, Zeta toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAT7K1AGeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD7K1AGeneGuard vector cassette: dapA, ColE2 COR, Kid toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA12DKR-AGeneGuard genomic cassette: for constructing ΔdapA::RBS A pi, Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support pSEVAD1K.Genomic cassette (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA12DKR-EGeneGuard genomic cassette: for constructing ΔdapA::RBS E pi, Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support pSEVAD1K.Genomic cassette (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA12DKR-JGeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.Genomic cassette (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA12TEC-BGeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.Genomic cassette (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA12TEC-IGeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.Genomic cassette (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
p005kanRFPRFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant; to be used in practicalsyntheticbiology.net/resourcesRed fluorescent protein (Other)Bacterial Expression, Synthetic Biology Bryk Practical synthetic biology (unpublished)
pSRKBB-emptyContains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.Bacterial Expression Schmidt-dannert BioBrick™ compatible plasmids for Rhodococcus (unpublished)
pCAP01The function of this material is to facilitate the capture and expression of bacterial gene clusters.Bacterial Expression, Yeast Expression, Synthetic Biology ; transformation-associated recombination cloning Moore Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1957-62. doi: 10.1073/pnas.1319584111. Epub 2014 Jan 21.
pBAM1This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome Synthetic Biology de Lorenzo pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes. BMC Microbiol. 2011 Feb 22;11:38. doi: 10.1186/1471-2180-11-38.
pAE03To construct C-terminal GFP fusions, single crossover integration vectorBacterial Expression, Synthetic Biology Veening Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems. Mol Microbiol. 2009 Oct;74(2):395-408. doi: 10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
pJWV25To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaABacterial Expression, Synthetic Biology Veening Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems. Mol Microbiol. 2009 Oct;74(2):395-408. doi: 10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
pPEP1BglFusion compatible integration vector for Streptococcus pneumoniaeBacterial Expression, Synthetic Biology Veening Gene Expression Platform for Synthetic Biology in the Human Pathogen Streptococcus pneumoniae. ACS Synth Biol. 2014 Jun 4.
pCM433kanTSucrose counterselection (sacB) vector for unmarked allelic exchange.Bacterial Expression, Synthetic Biology ; Sucrose counterselection Lidstrom Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense. Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
pAWP78Small IncP-based broad host range replicating vector.Bacterial Expression, Synthetic Biology Lidstrom Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense. Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
pAWP89IncP-based replicating vector expressing dTomato under tac promoter.Ptac-dTomatoBacterial Expression, Synthetic Biology Lidstrom Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense. Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
pMU102Cloning and expression vector for Clostridium thermocellumBacterial Expression, Synthetic Biology Lynd Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles. J Biol Eng. 2012 May 11;6(1):5. doi: 10.1186/1754-1611-6-5.
MXS_chaining vectorEmpty BackboneSynthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_MCS1MXS_chaining vector with MCS1EcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_MCS2MXS_chaining vector with MCS2EcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_MCS3MXS_chaining vector with MCS3MluI-XhoI-HindIII-PstI-SalI MCS (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_MCS4MXS_chaining vector with MCS4MluI-XhoI-PvuII-BglII-SalI MCS (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
SMX DEST vectorEmpty BackboneSynthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_TagBFPMXS_chaining vector with TagBFPTagBFP (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CeruleanMXS_chaining vector with CeruleanCerulean (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_LSS-mKate2MXS_chaining vector with LSS-mKate2LSS-mKate2 (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_mEGFPMXS_chaining vector with mEGFPmEGFP (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_mAzamiGreenMXS_chaining vector with mAzamiGreenmAzamiGreen (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CitrineMXS_chaining vector with CitrineCitrine (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_mKO2MXS_chaining vector with mKO2mKO2 (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_tdTomatoMXS_chaining vector with tdTomatotdTomato (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_mCherryMXS_chaining vector with mCherrymCherry (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_mPlumMXS_chaining vector with mPlummPlum (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_E2-CrimsonMXS_chaining vector with E2-CrimsonE2-Crimson (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_iRFP670MXS_chaining vector with iRFP670iRFP670 (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PA-GFPMXS_chaining vector with PA-GFPPA-GFP (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_KaedeMXS_chaining vector with KaedeKaede (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_H2BMXS_chaining vector with H2BHistone 2B with leading Kozak sequence (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_P2AMXS_chaining vector with P2ASelf-cleaving peptide (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::HygroR-bGHpAMXS_chaining vector with CMV::HygroR-bGHpA resistance cassette against hygromycin B with CMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::HygroR-bGHpAMXS_chaining vector with PGK::HygroR-bGHpA resistance cassette against hygromycin B with PGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::NeoR-bGHpAMXS_chaining vector with CMV::NeoR-bGHpA resistance cassette against Neomycin with CMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::NeoR-bGHpAMXS_chaining vector with PGK::NeoR-bGHpA resistance cassette against Neomycin with PGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::PuroR-bGHpAMXS_chaining vector with CMV::PuroR-bGHpA resistance cassette against Puromycin with CMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::PuroR-bGHpAMXS_chaining vector with PGK::PuroR-bGHpA resistance cassette against Puromycin with PGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::ZeoR-bGHpAMXS_chaining vector with CMV::ZeoR-bGHpA resistance cassette against Zeocin with CMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::ZeoR-bGHpAMXS_chaining vector with PGK::ZeoR-bGHpA resistance cassette against Zeocin with PGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
ColorfulCellMXS_chaining vector with 6 cassettes CMV::GTS-Citrine-bGHpA (Synthetic), CMV::Lyn-Ceruleanx3-bGHpA (Synthetic), CMV::NLS-TagBFPx3-bGHpA (Synthetic), CMV::MTS-AzamiGreen-bGHpA (Synthetic), CMV::iRFP670-SKL-bGHpA (Synthetic), CMV::ETS-mCherry-KDEL-bGHpA (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
ES-FUCCISMX_DEST vector with 3 cassettesminPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5 (Synthetic), minPGK::Cherry-hCdt1(30-120)-bGHpA (Synthetic), PGK::HygroR-bGpA (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
rtTA and TagBFP cassettesMXS_chaining vector with 2 cassettes CMV::NLS-TagBFPx3-bGHpA (Synthetic), CMV::rtTA3-bGHpA (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
EmptyTranscriptionalReporterMXS_chaining vector with 2 cassettes CMVmin::NLS-Citrine-bGHpA (Synthetic), CMV::NLS-Cherry-bGHpA (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
pHL391Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1gfp, mCherryBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL417Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 terminator + ColE1gfp, micC leader, hfq leaderBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL518Lim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1mCherry, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL671Lim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL718Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1gfp, mCherryBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL836Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1gfp, ipex leader, hfq leaderBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL1017Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL1632Lim Lab plasmid: AmpR + PLlacO-1::RBS(st7) gfp::Asp terminator + ColE1gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL1633Lim Lab plasmid: AmpR + PLlacO-1::T710 gfp::Asp terminator + ColE1gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL1954Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1M8::ryhB leader, hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL1969Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1M8::ryhB leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2021Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2026Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2027Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2053Lim Lab plasmid: AmpR + PLlacO-1::T710::hfq::T1 termintor + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2073Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2075Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2084Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1m8::glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2085Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1m8::glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2107Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1m8::micC leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2117Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1M8::ryhB leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pReporter_13_switchBPA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid.Reporter_13_switchBP (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pAV113pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV114pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV115pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV116pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV11KpRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV113.loxPpRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV115.loxPpRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV116.loxPpRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV123pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV124pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV125pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV126pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV103pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Yeast Expression, Worm Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV104pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV105pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV106pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.F3BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.F3.loxPpCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.F6.loxPpCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.K3.loxPpCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.K6.loxPpCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.K5.loxPpCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.HO3.loxPpCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.HO5.loxPpCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.KHpCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.KNpCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.K5pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.HO6pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.K4pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.Bacterial Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pUAP1Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.RFP cloning selection cassette (Synthetic)Synthetic Biology Patron Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytol. 2015 Jul 14. doi: 10.1111/nph.13532.
pEERM1 KmPlasmid for integration into psbA2 site of Synechocystis 6803Bacterial Expression, Synthetic Biology Lindberg Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide. ACS Synth Biol. 2015 Jul 13.
pEERM3 KmPlasmid for integration into neutral site of Synechocystis 6803Bacterial Expression, Synthetic Biology Lindberg Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide. ACS Synth Biol. 2015 Jul 13.
pEERM4 CmPlasmid for integration into neutral site 2 of Synechocystis 6803Bacterial Expression, Synthetic Biology Lindberg Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide. ACS Synth Biol. 2015 Jul 13.
pEERM6 KmPlasmid for integration into sqs site of Synechocystis 6803Bacterial Expression, Synthetic Biology Lindberg Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide. ACS Synth Biol. 2015 Jul 13.
pCAPB02A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilisBacterial Expression, Synthetic Biology Qian Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis. Sci Rep. 2015 Mar 24;5:9383. doi: 10.1038/srep09383.
J23100_ABMoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23100:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23100_EBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [E:J23100:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23100_FBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [F:J23100:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23100_GBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23102_ABMoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23102:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23102_EBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [E:J23102:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23102_FBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [F:J23102:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23102_GBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23102:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23103_ABMoClo Basic Part: Constitutive promoter - Anderson series - low strength [A:J23103:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23103_EBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [E:J23103:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23103_FBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [F:J23103:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23103_GBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23106_ABMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [A:J23106:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23106_EBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [E:J23106:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23106_FBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [F:J23106:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23106_GBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23106:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23107_ABMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [A:J23107:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23107_EBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [E:J23107:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23107_FBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [F:J23107:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23107_GBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23116_ABMoClo Basic Part: Constitutive promoter - Anderson series - low strength [A:J23116:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23116_EBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [E:J23116:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
J23116_GBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0010 (pLacI)_ABMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0010 (pLacI)_EBMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [E:R0010:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0010 (pLacI)_FBMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [F:R0010:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0010 (pLacI)_GBMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [G:R0010:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0040 (pTet)_ABMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0040 (pTet)_EBMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0040 (pTet)_FBMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [F:R0040:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0040 (pTet)_GBMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [G:R0040:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0063 (pLuxR-pR)_ABMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [A:R0063:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0063 (pLuxR-pR)_EBMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [E:R0063:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0063 (pLuxR-pR)_FBMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [F:R0063:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
R0063 (pLuxR-pR)_GBMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [G:R0063:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
I13453 (pBAD)_ABMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [A:I13453:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
I13453 (pBAD)_EBMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [E:I13453:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
I13453 (pBAD)_FBMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [F:I13453:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
I13453 (pBAD)_GBMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [G:I13453:B]Controllable promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
B0032m_BCMoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]RBS (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
B0033m_BCMoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C]RBS (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
B0034m_BCMoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C]RBS (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
BCD12_BCMoClo Basic Part: RBS - BiCistronic Design, medium strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD12:C]RBS (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
BCD2_BCMoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C]RBS (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
BCD8_BCMoClo Basic Part: RBS - BiCistronic Design, low strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD8:C]RBS (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
C0012m (lacI)_CDMoClo Basic Part: CDS - Controller protein, lacI repressor (in concert with CAP, represses pLacI, R0010). Modified to fix illegal site. [C:C0012m:D]Transcription factor (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
C0040 (tetR)_CDMoClo Basic Part: CDS - Controller protein, tetR repressor (represses pTet, C0040. can be inhibited by tetracyclin or aTc) [C:C0040:D]Transcription factor (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
C0062 (luxR)_CDMoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D]Transcription factor (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
C0080 (araC)_CDMoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]Transcription factor (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
cre_CDMoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP sites) [C:cre:D]Recombinase (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
E0030 (YFP)_CDMoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]Fluorescent reporter - YFP (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
E0040m (GFP)_CDMoClo Basic Part: CDS - Fluorescent protein. Green. Modified from Bba_E0040 to fix illegal site. [C:E0040m:D]Fluorescent reporter - GFP (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
E1010m (RFP)_CDMoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]Fluorescent reporter - RFP (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
eBFP2 (eBFP2)_CDMoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]Fluorescent reporter - BFP (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
B0015_DEMoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:E]Double Terminator (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
B0015_DFMoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:F]Double Terminator (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
B0015_DGMoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:G]Double Terminator (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
B0015_DHMoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:H]Double Terminator (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_ABMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:B]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_AEMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:E]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_AFMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:F]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_AGMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:G]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_AHMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:H]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_BCMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [B:LacZa:C]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_CDMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [C:LacZa:D]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_DEMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:E]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_DFMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:F]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_DGMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:G]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_DHMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:H]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_EBMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:B]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_EFMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:F]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_EGMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:G]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_EHMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:H]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_FBMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [F:LacZa:B]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVA_GBMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [G:LacZa:B]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVAMoClo Destination Vector: Empty vector for creating new DVA. Cut with SpeI and insert amplified LacZa (based on pSB1A2)None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pJ02B2Gm_AEMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]FACS Controls (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pJ02B2Gm_EFMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]FACS Controls (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pJ02B2Rm_AEMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]FACS Controls (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pJ02B2Rm_EFMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]FACS Controls (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pJ02B2Gm:Rm_AFMoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]FACS Controls (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pJ02B2Rm:Gm_AFMoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]FACS Controls (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pJ02B2Gm_AEMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]FACS Controls (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pJ02B2Gm_EFMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]FACS Controls (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pJ02B2Rm_AEMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]FACS Controls (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pJ02B2Rm_EFMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]FACS Controls (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVK_AEMoClo Destination Vector: (based on pSB1K3) [A:LacZa:E]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVK_AFMoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVK_EFMoClo Destination Vector: (based on pSB1K3) [E:LacZa:F]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVK_FGMoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVK_GHMoClo Destination Vector: (based on pSB1K3) [G:LacZa:H]None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
DVKMoClo Destination Vector: Empty vector for creating new DVK. Cut with SpeI and insert amplified LacZa (based on pSB1K3)None (Synthetic)Synthetic Biology ; Destination Vector Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
pCasper4-g-osk-TRICKoskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.oskar (Drosophila melanogaster)Insect Expression ; P element-mediated germline transformation Ephrussi Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
pTRK669Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.Bacterial Expression ; E. coli-gram positive bacteria shuttle vector Klaenhammer Efficient system for directed integration into the Lactobacillus acidophilus and Lactobacillus gasseri chromosomes via homologous recombination. Appl Environ Microbiol. 2001 Sep;67(9):4361-4.
pTKDP-neoDonor plasmid - kanamycin resistant donor cassetteBacterial Expression, Synthetic Biology Kuhlman An Integrated System for Precise Genome Modification in Escherichia coli. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
pTKDP-catDonor plasmid - chloramphenicol resistant donor cassetteBacterial Expression, Synthetic Biology Kuhlman An Integrated System for Precise Genome Modification in Escherichia coli. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
pTKDP-hphDonor plasmid - hygromycin resistant donor cassetteBacterial Expression, Synthetic Biology Kuhlman An Integrated System for Precise Genome Modification in Escherichia coli. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
pTKDP-dhfrDonor plasmid - trimethoprim resistant donor cassetteBacterial Expression, Synthetic Biology Kuhlman An Integrated System for Precise Genome Modification in Escherichia coli. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
pTKLP-tetAtetA landing pad template plasmidBacterial Expression, Synthetic Biology Kuhlman An Integrated System for Precise Genome Modification in Escherichia coli. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
pSS9Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coligfpuv (Synthetic)Synthetic Biology Gill Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli. ACS Synth Biol. 2016 Apr 22.
pAWp7-2pFUGW-UBCp-RFP-CMVp-GFP-[miR-128]UBCp-RFP-CMVp-GFP-[miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-14pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128]UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-2pBT264-[let-7b/miR-4763 cluster][let-7b/miR-4763 cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-3pBT264-[let-7c][let-7c] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-5pBT264-[let-7e/miR-99b cluster][let-7e/miR-99b cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-7pBT264-[miR-10b][miR-10b] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-8pBT264-[miR-126][miR-126] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-9pBT264-[miR-128b][miR-128b] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-10pBT264-[miR-129-2][miR-129-2] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-11pBT264-[miR-132][miR-132] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-13pBT264-[miR-16-1/15a cluster][miR-16-1/15a cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-14pBT264-[miR-181a][miR-181a] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-15pBT264-[miR-181c][miR-181c] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-16pBT264-[miR-188][miR-188] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-18pBT264-[miR-199b/3154 cluster][miR-199b/3154 cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-19pBT264-[miR-211][miR-211] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-20pBT264-[miR-212][miR-212] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-21pBT264-[miR-216][miR-216] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-24pBT264-[miR-29b][miR-29b] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-26pBT264-[miR-31][miR-31] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-28pBT264-[miR-328][miR-328] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-29pBT264-[miR-34a][miR-34a] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-31pBT264-[miR-376a][miR-376a] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-32pBT264-[miR-429][miR-429] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-33pBT264-[miR-451/144/4732 cluster][miR-451/144/4732 cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-34pBT264-[miR-488][miR-488] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-37pBT264-[miR-9-1][miR-9-1] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-38pBT264-[miR-93/106b cluster][miR-93/106b cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pTU1Backbone vector with internal BsaI removed by site-directed mutagenesisSynthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-A-lacZLevel 1 Destination vector - Position ALevel 1 - A site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-B-lacZLevel 1 Destination vector - Position BLevel 1 - B site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-C-lacZLevel 1 Destination vector - Position CLevel 1 - C site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-D-lacZLevel 1 Destination vector - Position DLevel 1 - D site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-A-RFPLevel 1 Destination vector - Position ALevel 1 - A site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-B-RFPLevel 1 Destination vector - Position BLevel 1 - B site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-C-RFPLevel 1 Destination vector - Position CLevel 1 - C site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-D-RFPLevel 1 Destination vector - Position DLevel 1 - D site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-D1-RFPLevel 1 Destination vector - Position D1Level 1 - D1 site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-E-RFPLevel 1 Destination vector - Position ELevel 1 - E site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU3-A-RFPLevel 3 Destination vector - Assembly of 2 Level 2 modules - ABLevel 3 - A site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU3-B-RFPLevel 3 Destination vector - Assembly of 4 Level 2 modules - ABCDLevel 3 - B site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBPBackbone vector with internal BsmBI removed by site-directed mutagenesisSynthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-lacZEntry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloningLevel 0 - Bioparts (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-ORFEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteLevel 0 - ORFs (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-A-lacZLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - A site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-B-lacZLevel 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - B site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-C-lacZLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - C site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-D-lacZLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - D site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-A-RFPLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - A site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-C-RFPLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - C site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-D-RFPLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - D site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-a-RFPLevel 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-ABLevel 2 - a site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-b-RFPLevel 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABCLevel 2 - b site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-ORF-eGFPLevel 0 Entry vector" ORF - eGFP "Enhanced green flourescent protein (eGFP) (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-ORF-eCFPLevel 0 Entry vector" ORF - eCFP"Enhanced cyan fluorescent protein (eCFP) (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-ORF-mCherryLevel 0 Entry vector" ORF - mCherry "mCherry fluorescent protein (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-J23100Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)J23100 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-J23108Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)J23108 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-J23114Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)J23114 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM901Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)SJM901 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM902Level 0 - SJM902 promoter (5' CTAT/3' GTAC fusion)SJM902 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM903Level 0 - SJM903 promoter (5' CTAT/3' GTAC fusion)SJM903 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM905Level 0 - SJM905 promoter (5' CTAT/3' GTAC fusion)SJM905 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM906Level 0 - SJM906 promoter (5' CTAT/3' GTAC fusion)SJM906 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM908Level 0 - SJM908 promoter (5' CTAT/3' GTAC fusion)SJM908 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM910Level 0 - SJM910 promoter (5' CTAT/3' GTAC fusion)SJM910 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM911Level 0 - SJM911 promoter (5' CTAT/3' GTAC fusion)SJM911 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM912Level 0 - SJM912 promoter (5' CTAT/3' GTAC fusion)SJM912 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM914Level 0 - SJM914 promoter (5' CTAT/3' GTAC fusion)SJM914 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM915Level 0 - SJM915 promoter (5' CTAT/3' GTAC fusion)SJM915 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-T7T7 promoter (5' CTAT/3' GTAC fusion)T7 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-T7_RBST7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)T7+RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-T7_RBS-His6-ThrombinT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)T7+RBS+His6+thrombin (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP_BBa_B0034Bba_B0034 iGEM RBS (5' GTAC/3' CATA fusion)Bba_B0034 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-pET-RBSPET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)pET RBS derived from pET vectors (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-Tag_linkerN-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion)N-terminal linker with pET-RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-HexHisHexahistidine N-terminal tag (5' TAAA/3' CATA fusion)Polyhistidine (HHHHHH) N-terminal tag (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-Strep(II)Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)Strep(II) N-terminal tag (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL1RBS_H1H1 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL2 RBS_H2H2 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL3RBS_H3H3 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL4RBS_H5H5 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL5RBS_M2M2 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL6RBS_M3M3 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL7RBS_M5M5 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL8RBS_L1L1 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL9RBS_L2L2 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL10RBS_L3L3 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL11RBS_L4L4 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL12RBS_L5L5 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-BBa_B0012BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)Bba_B0012 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-BBa_B0015BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)BBa_B0015 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L3S2P21L3S2P21 terminatorL3S2P21 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L3S1P51L3S1P51 terminatorL3S1P51 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L3S1P32L3S1P32 terminatorL3S1P32 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L3S1P11L3S1P11 terminatorL3S1P11 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L2U8H11L2U8H11 terminatorL2U8H11 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L2U5H11L2U5H11 terminatorL2U5H11 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L2U5H08L2U5H08 terminatorL2U5H08 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L2U3H03L2U3H03 terminatorL2U3H03 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L2U2H09L2U2H09 terminatorL2U2H09 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L1U1H09L1U1H09 terminatorL1U1H09 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L1U1H08L1U1H08 terminatorL1U1H08 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2_2aSecondary module (BpiI) site in pTU2a backboneSecondary module + Golden Gate pTU2a BsmBI destination site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2_2bSecondary module (BpiI) site in pTU2b backboneSecondary module + Golden Gate pTU2b BsmBI destination site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pCfB2899(X-2 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3034(X-3 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3035(X-4 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3036(XI-1 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB2903(XI-2 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB2904(XI-3 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3037(XI-5 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3038(XII-1 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3039(XII-2 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3040(XII-4 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB2909(XII-5 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pTU2S-a (p15A origin)Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationGolden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2S-b (p15A origin)Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-A-RFP (p15A origin)pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replicationGolden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2S-a (colE1 origin)Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationGolden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2S-b (colE1 origin)Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationGolden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-A-RFP (colE1 origin)pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-Bba_K822000L-Lactate inducible promoter from E. coli MG1655Bba_K822000 (L-Lactate promoter - E. coli MG1655) (Other)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-BBa_J23100BBa_J23100 promoter (iGEM collection)Bba_J23100 constitutive promoter (Sigma70) (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL9TL9 RBS (AAAAAG)TL9 RBS (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
p5E--3mnx1Can be used to drive expression specifically in zebrafish motor neuronsmotor neuron and pancreas homeobox 1 promoter (Danio rerio)5' entry vector for Multisite Gateway Three Fragment vector construction kit(Invitrogen) Cole A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Zebrafish. 2016 Sep 15.
p5E-gfapCan be used to drive expression in zebrafish astrocytes.glial fibrillary acidic protein promoter (Danio rerio)5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen) Cole A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Zebrafish. 2016 Sep 15.
pdCas9 (GB1079)Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusionsCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised) (Other)Plant Expression, CRISPR, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.hCas9 (Other)Plant Expression, CRISPR, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
pEGB 35s:dCas:BRD:tNos (GB1172)Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional RepressordCas9:BRD (Synthetic)Plant Expression, CRISPR, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
pEGB 35s:dCas:VP64:tNos (GB1189)Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional ActivatordCas:VP64 (Synthetic)Plant Expression, CRISPR, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
pEGB 35s:dCas:EDLL:tNos (GB1190)Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional ActivatordCas:EDLL (Synthetic)Plant Expression, CRISPR, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
pU6-1 (GB1204)Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid partAtU6-1 promoter (Arabidopsis thaliana)Plant Expression, CRISPR, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
tRNA-gRNA position [D1_2] (GB1205)tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)tRNA-gRNA (Synthetic)Plant Expression, CRISPR, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
tRNA-gRNA position [2_n-1] (GB1206)tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)tRNA-gRNA position [2_n-1] (Synthetic)Plant Expression, CRISPR, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
tRNA-gRNA position [n] (GB1207)tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)tRNA-gRNA position [n] (Synthetic)Plant Expression, CRISPR, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
tRNA-gRNA position [M1_2] (GB1209)tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_2]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (3-part multiplexing)tRNA-gRNA position [M1_2] (Synthetic)Plant Expression, CRISPR, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)Module for the expression of the Renilla Luciferase with the silencing suppressor P19Renilla / P19 (Other)Plant Expression, Luciferase, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
pECM1Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt1 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT1Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt1 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT1BPlasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt1B (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT1DPlasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt1D (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT1EPlasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt1E (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT3Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt3 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)Transcriptional unit for kanamycin resistance geneNptII (Other)Plant Expression, Synthetic Biology Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-insNuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).Histone H2B - Citrine (Homo sapiens)Mammalian Expression, Synthetic Biology Elowitz Dynamics of epigenetic regulation at the single-cell level. Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
MXS_BidirectionalCAGBidirectional CAG promoter (compatible with the MXS Chaining Kit)Bidirectional CAG Promoter (Synthetic)Mammalian Expression, Synthetic Biology Neveu Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells. PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016.
36INS-J23100_ABInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B]Insulated J23100 Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS-J23100_EB36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]Insulated J23100 Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS-J23100_FBInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B]Insulated J23100 Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS-J23100_GB36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]Insulated J23100 Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS1-pBAD_ABInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B]Insulated pBAD Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS1-pBAD_EBInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [E:36INS1-pBAD:B]Insulated pBAD Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS1-pBAD_FBInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [F:36INS1-pBAD:B]Insulated pBAD Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS1-pBAD_GBInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [G:36INS1-pBAD:B]Insulated pBAD Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS2-pBAD_ABInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]Insulated pBAD Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS2-pBAD_EBInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]Insulated pBAD Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS2-pBAD_FBInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]Insulated pBAD Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS2-pBAD_GBInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]Insulated pBAD Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS-pTet_ABInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [A:36INS-pTet:B]Insulated pTet Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS-pTet_EBInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [E:36INS-pTet:B]Insulated pTet Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS-pTet_FBInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [F:36INS-pTet:B]Insulated pTet Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)
36INS-pTet_GBInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [G:36INS-pTet:B]Insulated pTet Promoter (Synthetic)Synthetic Biology Densmore Insulated E. coli promoters (unpublished)

Shuttle Vectors

Search the table by keyword or sort by the table headings. Click on the publication link to view all other plamsids from that article. Click on the PI link to view all available plasmids from this depositor's lab.

Plasmid Purpose Vector Type Selectable Marker PI Publication
pPS311Yeast ExpressionLEU2 Silver A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm. Mol Cell Biol. 1994 Dec . 14(12):8399-407.
p405MET25Bacterial Expression, Yeast ExpressionLEU2 Buchler Buchler lab plasmids (unpublished)
p405MET3Bacterial Expression, Yeast ExpressionLEU2 Buchler Buchler lab plasmids (unpublished)
p405GAL1Bacterial Expression, Yeast ExpressionLEU2 Buchler Buchler lab plasmids (unpublished)
p405GALLBacterial Expression, Yeast ExpressionLEU2 Buchler Buchler lab plasmids (unpublished)
p405GALSBacterial Expression, Yeast ExpressionLEU2 Buchler Buchler lab plasmids (unpublished)
p405ADH1Bacterial Expression, Yeast ExpressionLEU2 Buchler Buchler lab plasmids (unpublished)
p405CYC1Bacterial Expression, Yeast ExpressionLEU2 Buchler Buchler lab plasmids (unpublished)
p405TEF1Bacterial Expression, Yeast ExpressionLEU2 Buchler Buchler lab plasmids (unpublished)
p405TDH3Bacterial Expression, Yeast ExpressionLEU2 Buchler Buchler lab plasmids (unpublished)
p404ADH1Bacterial Expression, Yeast ExpressionTRP1 Buchler Buchler lab plasmids (unpublished)
p404CYC1Bacterial Expression, Yeast ExpressionTRP1 Buchler Buchler lab plasmids (unpublished)
p404TEF1Bacterial Expression, Yeast ExpressionTRP1 Buchler Buchler lab plasmids (unpublished)
p404TDH3Bacterial Expression, Yeast ExpressionTRP1 Buchler Buchler lab plasmids (unpublished)
p406ADH1Bacterial Expression, Yeast ExpressionURA3 Buchler Buchler lab plasmids (unpublished)
p406CYC1Bacterial Expression, Yeast ExpressionURA3 Buchler Buchler lab plasmids (unpublished)
p406TEF1Bacterial Expression, Yeast ExpressionURA3 Buchler Buchler lab plasmids (unpublished)
p406TDH3Bacterial Expression, Yeast ExpressionURA3 Buchler Buchler lab plasmids (unpublished)
pESC-leu2d(empty)Yeast Expression, Synthetic Biology Keasling Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006 Apr 13. 440(7086):940-3.
pRBBm34Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megateriumBacterial Expression, Synthetic Biology Jahn A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. J Biotechnol. 2007 Dec 1;132(4):426-30. Epub 2007 Jul 10.
pRBBm59Shuttle vector E. coli/B.meg.Bacterial Expression, Synthetic Biology Jahn A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. J Biotechnol. 2007 Dec 1;132(4):426-30. Epub 2007 Jul 10.
pRBBm69Shuttle vector E. coli/B.meg.Bacterial Expression, Synthetic Biology Jahn A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. J Biotechnol. 2007 Dec 1;132(4):426-30. Epub 2007 Jul 10.
pRBBm76Shuttle vector E. coli/B.meg.Bacterial Expression, Synthetic Biology Jahn A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. J Biotechnol. 2007 Dec 1;132(4):426-30. Epub 2007 Jul 10.
pSPYngK-hpShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter Bacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pSPVpr-hp Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoterBacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pSPNprM-hpShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter Bacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pSPYngK+-hpShuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterBacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pSPAsp-hpShuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter Bacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pSPPac-hpShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoterBacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pPSP6+t-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoterBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-1+t-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-2+t-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-1-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pHBintTShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorBacterial Expression, Synthetic Biology Jahn Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microb Biotechnol. 2010 Jan;3(1):24-37. doi: 10.1111/j.1751-7915.2009.00125.x. Epub 2009 Jun 10.
pHBintNShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vector Bacterial Expression, Synthetic Biology Jahn Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microb Biotechnol. 2010 Jan;3(1):24-37. doi: 10.1111/j.1751-7915.2009.00125.x. Epub 2009 Jun 10.
pHBintEShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector Bacterial Expression, Synthetic Biology Jahn Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microb Biotechnol. 2010 Jan;3(1):24-37. doi: 10.1111/j.1751-7915.2009.00125.x. Epub 2009 Jun 10.
pSP6-RNAPShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylABacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pK1E-RNAPShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylABacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-1Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-2Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-1+tShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-2+tShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPSP6+tShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6Bacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPSP6Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6Bacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pNtCp-rbcL-accDContains rbcL and accD DNA regions for double homologous recombination useful in Nicotiana tabacum Cp transformationBacterial Expression, Synthetic Biology Melis Melis lab pNtCp-rbcL-accD (unpublished)
pBS1CEmpty vector, integration at amyE, ampr, cmrSynthetic Biology ; Bacillus BioBrick Boxchloramphenicol resistance in B. subtilis Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
pBS2EEmpty vector, integration at lacA, ampr, mlsrSynthetic Biology ; Bacillus BioBrick Boxerm: specifies resistance to macrolide, lincosamide and streptogramin B antibiotics if induced by erythromycin Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
pBS4SEmpty vector, integration at thrC, ampr, specrSynthetic Biology ; Bacillus BioBrick Boxspectinomycin resistance in B. subtilis Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
pBS1ClacZlacZ-reporter vector, integration at amyE, ampr, cmrSynthetic Biology ; Bacillus BioBrick Boxchloramphenicol resistance in B. subtilis Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
pBS3Cluxlux-reporter vector, integration at sacA, ampr, cmrSynthetic Biology ; Bacillus BioBrick Boxchloramphenicol resistance in B. subtilis Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
pTHDual-expression vector for P. tricornutumSynthetic Biology ; Diatom Expression Weyman Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis. Plant Biotechnol J. 2014 Oct 10. doi: 10.1111/pbi.12254.
pSEVA3b61Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA3b67RbShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pTE100MethyloBrick cloning vector, no promoter; TcRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pTE101MethyloBrick cloning vector, no promoter; KmRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pTE102MethyloBrick expression vector, mxaF promoter; TcRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pTE103MethyloBrick expression vector, fumC promoter; TcRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pTE104MethyloBrick expression vector, coxB promoter; TcRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pTE105MethyloBrick expression vector, tuf promoter; TcRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pC-G418-YRYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).Ternary vector for Agrobacterium mediated transformation of fungiNeomycin (select with G418), URA3 Haynes Exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in Zymoseptoria tritici. Fungal Genet Biol. 2015 Jun;79:102-9. doi: 10.1016/j.fgb.2015.04.015.
pDS2CEN/ARS yeast shuttle vector with DS2 selection markerSynthetic Biology Sommer Flexible metabolic pathway construction using modular and divisible selection gene regulators. Metab Eng. 2015 Sep;31:189-97. doi: 10.1016/j.ymben.2015.08.004. Epub 2015 Aug 21.
pG1AKModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG1AK-mCherryModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG1AK-PheBModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG1AK-sfGFPModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG1CModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG1KModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG2KModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.

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