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SynBio_Icon_Metabolism.png Synthetic Biology: Metabolism


Browse Addgene's collection of synthetic biology plasmids related to metabolic pathways and components.

Examples Include:

  • Biofuels
  • Natural Product Pathways
  • Efflux Pumps
  • Flux Control

Metabolism Plasmids

Search the table by keyword or sort by the table headings. Click on the publication link to view all plamsids available from the article. Click on the PI link to view all plasmids available from this depositor's lab.

Plasmid Gene/Insert Vector Type Promoter PI Publication Hidden Extra Search Info  
pBAD33MevTMevT (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802. pBAD33 Add to Cart
pMevT (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802. n/a Add to Cart
pMBImevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802. n/a Add to Cart
pMBISmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802. n/a Add to Cart
pMevBmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802. n/a Add to Cart
pBAD24MevTMevT (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli. Metab Eng. 2007 Mar . 9(2):193-207. pBAD24 Add to Cart
pBdxsdxsBacterial Expression, Synthetic Biology Keasling Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol Bioeng. 2001 Feb 20. 72(4):408-15. pBluescript Add to Cart
pBAD*RFPEC2E. coli optimized red flourescent protein (RFPec2)Bacterial Expression, Synthetic Biology Keasling Optimization of DsRed production in Escherichia coli: effect of ribosome binding site sequestration on translation efficiency. Biotechnol Bioeng. 2005 Dec 5. 92(5):553-8. pBAD24 Add to Cart
pET30-R5-GFPpET30-GFPBacterial Expression, Synthetic Biology Keasling Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein. Anal Chem. 2008 Feb 1. 80(3):886-90. pET30 Add to Cart
pAC-PHYTcrtE, crtBBacterial Expression, Synthetic Biology Keasling Controlling the metabolic flux through the carotenoid pathway using directed mRNA processing and stabilization. Metab Eng. 2001 Oct . 3(4):313-21. pAC-LYC Add to Cart
pADScodon-optimized synthetic amorphadiene synthaseBacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802. pTrc99a Add to Cart
pBAD18HMGRtruncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli. Metab Eng. 2007 Mar . 9(2):193-207. pBAD18 HMG1 YML075C Add to Cart
pSB77thiosulfate reductaseBacterial Expression, Synthetic Biology Keasling Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli. Appl Environ Microbiol. 2000 Sep . 66(9):3939-44. pJB866 Add to Cart
pSB74thiosulfate reductaseBacterial Expression, Synthetic Biology Keasling Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli. Appl Environ Microbiol. 2000 Sep . 66(9):3939-44. pTrc99A phsB Add to Cart
pDUET-ctCPR-nAMOp450 reductase and native Amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7. pETDuet-1 Add to Cart
pDUET-ctCPR-sAMOp450 reductase and codon optimized amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7. pETDuet-1 Add to Cart
pDUET-ctCPR-trAMOp450 reductase and truncated amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7. pETDuet-1 Add to Cart
pDUET-ctCPR-BovAMOp450 reductase, and amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7. pETDuet-1 Add to Cart
pDUET-ctCPR-A13AMOp450 reductase and amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7. pETDuet-1 Add to Cart
pDUET-ctCPR-A17AMOp450 reductase and amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7. pETDuet-1 Add to Cart
pDUET-aaCPRct-A13AMOp450 reductase and Amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7. pETDuet-1 Add to Cart
pCWori-A13AMO-aaCPRctAmorphadiene oxidase and p450 reductaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7. pCWori Add to Cart
pESC-URA::CYP71AV1/CPRamorphadiene oxidase and p450 reductaseYeast Expression, Synthetic Biology Keasling Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006 Apr 13. 440(7086):940-3. pESC-URA Add to Cart
pRS425ADSamorphadiene synthaseYeast Expression, Synthetic Biology Keasling Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006 Apr 13. 440(7086):940-3. pRS425GAL1 Add to Cart
pKS2'tesABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62. pBBR Add to Cart
pKS13pdc, adhBBacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62. pBBR Add to Cart
pKS17pdc, adhB, atfABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62. pBBR Add to Cart
pKS18mFar1 (Mus musculus)Bacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62. pBBR Far1 Mlstd2, AI850429, 2600011M19Rik, 2900034E22Rik, 3732409C05Rik Add to Cart
pKS100fadDBacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62. pBR322 Add to Cart
pKS101FAA2 (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62. pBR322 FAA2 FAM1 Add to Cart
pKS104fadD (M3351), atfABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62. ColE1? Add to Cart
pKS1'tesABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62. p15a Add to Cart
pGB3xynBBacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62. pPro Add to Cart
pBbA5c-MevT-MBISAtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic BiologyLacUV5, Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pBbA5c Add to Cart
pBbA5c-MevT(CO)-MBIS(CO, ispA)AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic BiologyLacUV5, Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pBbA5c Add to Cart
pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic BiologyLacUV5, pTrc Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pBbA5c Add to Cart
pTrcAgBIS (CO)Bisabolene SynthaseBacterial Expression, Synthetic Biology pTrc Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pTrc99a Add to Cart
pRSLeu2d-BISoptBisabolene SynthaseYeast Expression, Synthetic BiologyP GAL 1 Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pRS425ADS-Leu2d Add to Cart
pAC-4CL14-coumaroyl CoA ligase 1 (Arabidopsis thaliana)Bacterial Expression, Synthetic BiologypLac-mod Schmidt-dannert Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli. BMC Biotechnol. 2006 Mar 21;6:22. pAC 4CL1 AT1G51680, 4-COUMARATE:COA LIGASE, 4-COUMARATE:COA LIGASE 1, 4-coumarate:CoA ligase 1, 4CL.1, ARABIDOPSIS THALIANA 4-COUMARATE:COA LIGASE 1, AT4CL1, F19C24.11, F19C24_11 Add to Cart
pUC-STSStilbene synthaseSynthetic BiologyLacPMod Schmidt-dannert Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli. BMC Biotechnol. 2006 Mar 21;6:22. pUCmod Add to Cart
pCellulosepCelluloseSynthetic BiologyTwo promoters from the E coli genome drive two different genes: PcspD and PwrbA Keasling Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19949-54. Epub 2011 Nov 28. Biobrick family of vectors Add to Cart
pXylanpXylanSynthetic BiologyTwo promoters from the E coli genome drive two different genes: PcspD and PcstA Keasling Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19949-54. Epub 2011 Nov 28. Biobrick family of vectors Add to Cart
pBA2kIKmA2Kudzu Isoprene Synthase (Synthetic)Synthetic BiologypsbA2 Melis Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13. pBluescript Add to Cart
pBA2SkIKmA2Synechocystis kudzu IspSSynthetic BiologypsbA2 Melis Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13. pBluescript Add to Cart
pAM1518psbAI promoter, luxCDECyanobacteria cloning vectorn/a Golden Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Methods Enzymol. 2000;305:527-42. pAM1504 Add to Cart
pBbA5k-EPL123AcrABBacterial Expression, Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL3mexF, Avin_33870 (Synthetic)Bacterial Expression, Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k mexF Avin_33870 Add to Cart
pBbA5k-EPL4Avin_44060 (Synthetic)Bacterial Expression, Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL11ttgB, PP_1385 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL12PP_3456 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL14mexF, PP_3426 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL15PP_0906 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL33PA_2018 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL36mexF, PA2494 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL37PA_4375 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL42PFL_1331 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL43PFL_3271 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL45PFL_1028 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL48PFL_1158 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL52Rmet_4866 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL54Rmet_1702 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL55Maqu_3494 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL56Maqu_0582 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL59PSPPH_4014 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL61PSPPH_2907 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL63mdtB, PSPPH_2641 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL64PSPPH_0733 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL65PSPPH_1196 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL66PSPPH_0329Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL68Gmet_1664 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL69Gmet_2518 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL70Gmet_1652 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL84BP_2076 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL90DVU_0438 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL95AcrB/AcrD/AcrFa, ABO_0964 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL98acrB, PSHAb_0324 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL100Shal_0198 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL101Shal_1195 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL102Shal_2167 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL122acrF (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pBbA5k-EPL124acrD (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. pBbA5k Add to Cart
pJBEI-6409Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology ; BglBrickPlacUV5 Lee Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli pBbA5c Add to Cart
pJBEI-6410Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology ; BglBrickPlacUV5 Lee Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli pBbA5a Add to Cart
pJBEI-6411ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase (Other)Bacterial Expression, Synthetic Biology ; BglBrickPbad Lee Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli pBbB8k Add to Cart
pS3aroE, aroD, aroB, aroG*, ppsA, tktABacterial Expression, Synthetic BiologylacUV5, lacUV5, lacUV5, lacUV5, lacUV5, lacUV5 Keasling Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21. Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5 pBbB5c Add to Cart
pY1noneBacterial Expression, Synthetic Biology ; Empty VectorPLacUV5 Keasling Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21. Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5, pRBS-01 Add to Cart
pTrc-trGPPS(CO)-LSLimonene Synthase, ApR, LacIqBacterial Expression, Synthetic BiologyTrc, Trc, Trc Keasling Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase E. coli DH10B Add to Cart
pY3tyrB, tyrA, aroC, aroA, aroLBacterial Expression, Synthetic BiologylacUV5, lacUV5, lacUV5, lacUV5, lacUV5 Keasling Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21. Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5 pRBS-01 Add to Cart
pET-Ce25arginyl-tRNA synthetase (Other)Bacterial ExpressionT7 Igloi Expression and properties of arginyl-tRNA synthetase from jack bean (Canavalia ensiformis). Protein Expr Purif. 2008 Oct;61(2):163-7. doi: 10.1016/j.pep.2008.05.008. Epub 2008 May 23. Expresses a plant arginyl-tRNA synthetase in E.coli pET32a+ Add to Cart
psbA2-PHLS (b)beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic BiologypsbA2 Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014 Replaces the psbA2 gene in Synechocystis with the PHLS gene pBluescript KS+ Add to Cart
psbA2(noATbox)-PHLS (c)beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic BiologypsbA2 Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014 Replaces the psbA2 gene in Synechocystis with the PHLS gene pBluescript KS+ Add to Cart
psbA2-Ptrc-PHLS (d)beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic BiologyPtrc Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014 Replaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS gene pBluescript KS+ Add to Cart
psbA2-Ptrc-T7-g10-PHLS (e)beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic BiologyPtrc-T7 Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014 Replaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS gene pBluescript KS+ Add to Cart
cpc-PHLS (g)beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic BiologySynechocystis endogenous cpc-operon promoter Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014 Replaces the cpc operon in Synechocystis with the PHLS gene pBluescript KS+ Add to Cart
pNtCp-rbcL-PHLS-aadA-accDrbcL-PHLS-aadA-accD (Other)Bacterial Expression, Synthetic Biology Melis Melis lab pNtCp-rbcL-accD (unpublished) Inserts the beta-phellandrene synthase gene and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum pBluescript II KS+ Add to Cart
pNtCp-rbcL-PHLS-GPPS-aadA-accDrbcL-PHLS-GPPS-aadA-accD (Other)Bacterial Expression, Synthetic Biology Melis Melis lab pNtCp-rbcL-accD (unpublished) Inserts the beta-phellandrene synthase gene, the geranyl diphosphate synthase gene, and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum pBluescript II KS+ Add to Cart
pET-Gm1arginyl-tRNA synthetase (Other)Bacterial ExpressionT7 Igloi Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants. FEBS J. 2009 Mar;276(5):1307-18. doi: 10.1111/j.1742-4658.2009.06866.x. Expresses a plant arginyl-tRNA synthetase in E.coli pET32a+ Add to Cart
pET-Cb2mitochondrial arginyl-tRNA synthetase (Other)Bacterial ExpressionT7 Igloi Amino acid discrimination by the nuclear encoded mitochondrial arginyl-tRNA synthetase of the larva of a bruchid beetle (Caryedes brasiliensis) from northwestern Costa Rica. Insect Biochem Mol Biol. 2013 Dec;43(12):1172-80. Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli pET32a+ Add to Cart
pEU-Gm23arginyl-tRNA synthetase (Other)Synthetic BiologySP6 Igloi Wheat germ in vitro translation of soybean arginyl-tRNA synthetase (unpublished) Used for in vitro transcription/translation of soybean His-tagged arginyl-tRNA synthetase in the Cell Free Sciences system pEU-E01 Add to Cart
pBbE1k-2dihydropterine reductase, pterin-4a-carbinolamine dehydratasepTrc, pTrc Lee Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29. dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human pBbE1k Add to Cart
pBAMD1-2Synthetic Biology ; Mini-Tn5 vector de Lorenzo New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014. Standardized mini-Tn5 delivery plasmid, kanamycin resistant pBAMD1-2 Add to Cart
pBAMD1-4Synthetic Biology ; Mini-Tn5 vector de Lorenzo New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014. Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant pBAMD1-4 Add to Cart
pBAMD1-6Synthetic Biology ; Mini-Tn5 vector de Lorenzo New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014. Standardized mini-Tn5 delivery plasmid, gentamicin resistant pBAMD1-6 Add to Cart
p148flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7 Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR pETM6 Add to Cart
p158flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7 Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR pETM6 Add to Cart
p168flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7 Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR pETM6 Add to Cart
p168-759flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), Scaffold Protein encoding GBD:SH3:PDZ (1:1:4) (Synthetic)Bacterial Expression, Synthetic BiologyT7, T7, T7, T7 Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4) pETM6 Add to Cart
p1585588flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), dihydroflavonol 4-reductase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7, T7, T7, T7, T7 Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio) pETM6 Add to Cart
LAMP1-ABKARABKAR biosensorMammalian ExpressionCMV Inoue Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators Cell Reports Expression of ABKAR biosensor targeted to the lysozome pcDNA3 Add to Cart
pDCAF3-IS5ndmA (Other), ndmB (Other), ndmC (Other), ndmD (Other), gst9 (Other), chlR (Other)Bacterial Expression, Synthetic BiologyBBa_J23100, BBa_J23100, BBa_J23100, BBa_J23100, BBa_J23100 Barrick Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5. ACS Synth Biol. 2013 Jun 21;2(6):301-7. doi: 10.1021/sb4000146. Epub 2013 Mar 22. Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below. pSB1C3 Add to Cart
HcKan_PRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of promoters for the construction of standard promoter parts in YeastFab work pSMART HcKan Add to Cart
HcKan_ORFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of ORFs for the construction of standard ORF parts in YeastFab work pSMART HcKan Add to Cart
HcKan_TRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of terminators for the construction of standard terminator parts in YeastFab work pSMART HcKan Add to Cart
POT2-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly pRS416 Add to Cart
POT3-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly pRS416 Add to Cart
POT4-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly pRS416 Add to Cart
POT5-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly pRS416 Add to Cart
POT6-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly pRS416 Add to Cart
POT7-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly pRS416 Add to Cart
POT8-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly pRS416 Add to Cart
POT9-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly pRS416 Add to Cart
POT10-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly pRS416 Add to Cart
POT11-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly pRS416 Add to Cart
pRS414-URRURR1-RFP-Leu2-URR2 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. CEN plasmid as receiving vector for exogenous pathway pRS414 Add to Cart
pRS424-URRURR1-RFP-Leu2-URR2 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. high copy number plasmid as receiving vector for exogenous pathway pRS424 Add to Cart
pMV-URR1URR1 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs pMV Add to Cart
pMV-URR2URR2 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs pMV Add to Cart
pMV-HIS3HIS3 (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs pMV Add to Cart
pMV-LEU2LEU2 (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs pMV Add to Cart
pMV-TRP1TRP1 (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs pMV Add to Cart
HCKan-P-TDH3TDH3 promoter (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. construction of TDH3 promoter in HCKan-P receiving vector HCKan-P Add to Cart
HCKan-P-TEF2TEF2 promoter (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. construction of TEF2 promoter in HCKan-P receiving vector HCKan-P Add to Cart
HCKan-P-CYC1CYC1 promoter (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. construction of CYC1 promoter in HCKan-P receiving vector HCKan-P Add to Cart
HCKan-T-ADH1ADH1 terminator (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. construction of ADH1 terminator in HCKan-P receiving vector HCKan-T Add to Cart
HCKan-T-TEF2TEF2 terminator (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. construction of TEF2 terminator in HCKan-P receiving vector HCKan-T Add to Cart
PMV-ycrtEycrtE (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. systhesis of crtE gene in the beta-carotene pathway PMV Add to Cart
PMV-ycrtIycrtI (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. systhesis of crtI gene in the beta-carotene pathway PMV Add to Cart
PMV-ycrtYBycrtYB (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. systhesis of crtYB gene in the beta-carotene pathway PMV Add to Cart
HCKan-O-ycrtEycrtE (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. construction of ycrtE gene in HCKan-O receiving vector HCKan-O Add to Cart
HCKan-O-ycrtIycrtI (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. construction of ycrtI gene in HCKan-O receiving vector HCKan-O Add to Cart
HCKan-O-ycrtYBycrtYB (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. construction of ycrtYB gene in HCKan-O receiving vector HCKan-O Add to Cart
pKMV-VioAvioA (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. systhesis of vioA gene in the violacein pathway pKMV Add to Cart
pKMV-VioBvioB (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. systhesis of vioB gene in the violacein pathway pKMV Add to Cart
pKMV-VioCvioC (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. systhesis of vioC gene in the violacein pathway pKMV Add to Cart
pKMV-VioDvioD (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. systhesis of vioD gene in the violacein pathway pKMV Add to Cart
pKMV-VioEvioE (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464. systhesis of vioE gene in the violacein pathway pKMV Add to Cart
pKS042clpABacterial Expression, Synthetic Biologytrc Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016. pTrc plasmid expressing clpA pTrcHisB clpA b0882, ECK0873, JW0866, lopD Add to Cart
pKS043clpSBacterial Expression, Synthetic Biologytrc Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016. pTrc plasmid expressing clpS pTrcHisB clpS b0881, ECK0872, JW0865, yljA Add to Cart
pKS044clpPBacterial Expression, Synthetic Biologytrc Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016. pTrc plasmid expressing clpP pTrcHisB clpP b0437, ECK0431, JW0427, lopP, wseA Add to Cart
PAS501 (TJF040)FadD with Q338R mutation (Other)Bacterial ExpressionT7/lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD Q338R pETDuet-1 Add to Cart
PAS500 (TJF032)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing WT FadD pETDuet-1 Add to Cart
PAS502 (TJF065)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD H376R pETDuet-1 Add to Cart
PAS503 (TJF066)FadD with V4F, W5L mutations (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD V4F, W5L pETDuet-1 Add to Cart
PAS504 (TJF067)FadD with F447S mutation (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD F447S pETDuet-1 Add to Cart
PAS505 (TJF068)FadD with V451A mutation (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD V451A pETDuet-1 Add to Cart
PAS506 (TJF069)FadD D372G (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD D372G pETDuet-1 Add to Cart
PAS507 (TJF070)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD Y9H pETDuet-1 Add to Cart
PAS508 (TJF071)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD T349A pETDuet-1 Add to Cart
PAS509 (TJF032 L448S)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD L448S pETDuet-1 Add to Cart
PAS510 (TJF032 G446S)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD G446S pETDuet-1 Add to Cart
PAS511 (TJF032 Y375R)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD Y375R pETDuet-1 Add to Cart
PAS512 (TJF032 S377R)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD S377R pETDuet-1 Add to Cart
PAS513 (TJF080)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD S379R pETDuet-1 Add to Cart
PAS514 (TJF081)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD F447D pETDuet-1 Add to Cart
PAS515 (TJF082)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD F447R pETDuet-1 Add to Cart
PAS516 (TJF083)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD F447R S379R pETDuet-1 Add to Cart
PAS517 (TJF084)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD F447D S379R pETDuet-1 Add to Cart
PAS518 (TJF085)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD R554G pETDuet-1 Add to Cart
PAS519 (TJF086)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD D551G pETDuet-1 Add to Cart
PAS520 (TJF087)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD Q338G pETDuet-1 Add to Cart
PAS521 (TJF088)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD R449G pETDuet-1 Add to Cart
PAS522 (TJF089)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD V451G pETDuet-1 Add to Cart
PAS523 (TJF090)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD H376G pETDuet-1 Add to Cart
PAS524 (TJF091)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD S379G pETDuet-1 Add to Cart
PAS525 (TJF092)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD Q339G pETDuet-1 Add to Cart
PAS526 (TJF093)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD L459G pETDuet-1 Add to Cart
PAS527 (TJF094)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD Y371G pETDuet-1 Add to Cart
PAS528 (TJF059)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD-His pETDuet-1 Add to Cart
PAS529 (TJF072)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD Q338R-His pETDuet-1 Add to Cart
PAS530 (TJF073)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD H376R-His pETDuet-1 Add to Cart
PAS531 (TJF074)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD V4F, W5L -His pETDuet-1 Add to Cart
PAS532 (TJF075)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD F447S-His pETDuet-1 Add to Cart
PAS533 (TJF076)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD V451A-His pETDuet-1 Add to Cart
PAS534 (TJF077)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD D372G-His pETDuet-1 Add to Cart
PAS535 (TJF078)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD Y9H-His pETDuet-1 Add to Cart
PAS536 (TJF079)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015. pETDuet-1 Containing FadD T349A-His pETDuet-1 Add to Cart
pETM6-C4-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - C4 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. Expresses mCherry under the control of Mutant T7 Promoter C4 pETM6 Add to Cart
pETM6-H10-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - H10 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. Expresses mCherry under the control of Mutant T7 Promoter H10 pETM6 Add to Cart
pETM6-H9-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - H9 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. Expresses mCherry under the control of Mutant T7 Promoter H9 pETM6 Add to Cart
pETM6-G6-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - G6 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. Expresses mCherry under the control of Mutant T7 Promoter G6 pETM6 Add to Cart
pETM6-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyConsensus T7 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. Expresses mCherry under the control of the Concensus T7 Promoter pETM6 Add to Cart
pETM6-vioABECDVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic BiologyConsensus T7, Consensus T7, Consensus T7, Consensus T7, Consensus T7 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter pETM6 Add to Cart
pETM6-G6-vioABECDVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter pETM6 Add to Cart
pETM6-E12-vioABECDVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - H10, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - H10, Mutant T7 Promoter - G6 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12) pETM6 Add to Cart
pETM6-M.accABCDaccA (Other), accB (Other), accC (Other), accD (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7, T7 Koffas Modular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425. Encodes accA, accB, accC, accD in monocistronic configuration. pETM6 Add to Cart
pETM6-P.accABCDaccA (Other), accB (Other), accC (Other), accD (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7, T7 Koffas Modular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425. Encodes accA, accB, accC, accD in Pseudo-operon configuration. pETM6 Add to Cart
pWKS1583amyEopt (Synthetic)Bacterial Expression, Synthetic BiologyPtet Smits The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile. ACS Synth Biol. 2016 Jun 23. Modular vector for secreted amylase reporter expression pRPF185 Add to Cart
pETM6-HpaBCHpaB (Other), HpaC (Other)Bacterial Expression, Synthetic BiologyT7, T7 Koffas "Optimization of naringenin and p-coumaric acid hydroxylation using the native E. coli hydroxylase complex, HpaBC". Biotechnol Prog. 2015 Oct 21. doi: 10.1002/btpr.2185. Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6 pETM6 Add to Cart
pBbE1k-3dihydropterine reductase (Homo sapiens), pterin-4a-carbinolamine dehydratase (Homo sapiens)pTrc, pTrc Lee Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29. tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human pBbE1k QDPR DHPR, PKU2, SDR33C1 Add to Cart
pBbE1k-DDCdopa decarboxylase (Other)pTrc Lee Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29. dopa decarboxylase from pig pBbE1k Add to Cart
pJK108delta-12 fatty acid acetylenase (Other)Bacterial ExpressionT7 Kappock Additional fatty acid desaturase and acetylenase constructs (unpublished) Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic) pET23d Add to Cart
PsbA2-HMGS-HMGR-ATOB-NPTIHMGS-HMGR-ATOB-NPTI (Other)psbA2 Melis Expression of the MVA pathway in cyanobacteria (unpublished) Construct expressing the upper MVA pathway in Synechocystis PCC 6803 PUC57 Genescript Add to Cart
pETM6-G6-vioABEPG6-vioABE (Other)Bacterial Expression, CRISPR, Synthetic BiologyPG6 (orthogonal T7-lac variant) Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6. pETM6 Add to Cart
pETM6-G6-vioABE-4A6-vioCPG6-vioABE-P4A6-vioC (Other)Bacterial Expression, CRISPR, Synthetic BiologyPG6 and P4A6 (orthogonal T7-lac variants) Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection. pETM6 Add to Cart
pETM6-G6-vioABE-4A6-vioC-3A2-vioDPG6-vioABE-P4A6-vioC-P3A2-vioD (Other)Bacterial Expression, CRISPR, Synthetic BiologyPG6, P4A6, and P3A2 (orthogonal T7-lac variants) Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection. pETM6 Add to Cart
pJKR-H-benMmuconate biosensing apparatus Church Genetically encoded sensors enable real-time observation of metabolite production. Proc Natl Acad Sci U S A. 2016 Feb 8. pii: 201600375. Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell. pUC Add to Cart
pZ8-P_dCas9dcas9Bacterial Expression, CRISPR, Synthetic BiologyPropionate inducible promoter (prp) Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR pZ8-Prp Add to Cart
pZ8-PtacBacterial Expression, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR pZ8-1 Add to Cart
pAL-pgi_TsgRNA pgi (T)Bacterial Expression, CRISPR, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR pAL374 Add to Cart
pAL-pgi_NTsgRNA pgi (NT) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR pAL374 Add to Cart
pAL-pck_TsgRNA pck (T) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR pAL374 Add to Cart
pAL-pck_NTsgRNA pck (NT) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR pAL374 Add to Cart
pAL-pyk_TsgRNA pyk (T) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR pAL374 Add to Cart
pAL-pyk_NTsgRNA pyk (NT) (Synthetic)Bacterial Expression, CRISPRptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR pAL374 Add to Cart
pAL-rfp_TsgRNA rfp (T) (Synthetic)Bacterial Expression, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR pAL374 Add to Cart
PP259GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. Promoter: scTEF1, minimal Promoter: mCYC. Figure 4. Expresses GFP. pPICZ A Add to Cart
PP257GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. Promoter: GAP, minimal Promoter: mCYC. Figure 4. Expresses GFP. pPICZ A Add to Cart
PP245GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. Promoter: GAP, minimal Promoter: AOX1. Figure 4. Expresses GFP. pPICZ A Add to Cart
PP277GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. Promoter: scTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP. pPICZ A Add to Cart
PP190GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. Promoter: ppTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP. pPICZ A Add to Cart
PP273GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. Promoter: ppTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP. pPICZ A Add to Cart
PP160GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. Supplemental Figure 4. Promoter GAP2. Expresses GFP. pPICZ A Add to Cart
PP155GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. Supplemental Figure 4. Promoter GAP4. Expresses GFP. pPICZ A Add to Cart
PP163GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. Supplemental Figure 4. Promoter GAP6. Expresses GFP. pPICZ A Add to Cart

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