Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Synthetic Biology: Bacterial Strains


Browse Addgene's collection of synthetic biology related bacterial strains.




SynBio Bacterial Strains

Search the table by keyword or sort by the table headings. Click on the publication link to view all plamsids available from the article. Click on the PI link to view all plasmids available from this depositor's lab.

Plasmid Purpose Gene/Insert Antibiotic Resistance PI Publication
J72008 bacterial strain with plasmidphi80 integration helper plasmid pInt80-649Ampicillin Anderson BglBricks: A flexible standard for biological part assembly. J Biol Eng. 2010 . 4(1):1.
HL 818 (=HL 716 + pHL 98)pLlacO-1:DsrA-mcherry, pLtetO-1:rpoS::gfp (Other)Ampicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 839 (=HL 716 + pHL 108)pLlacO-1:MicC-mCherry, pLtetO-1:ompC::gfp (Other)Ampicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 849 (=HL 716 + micC knockout_KanR)Kanamycin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1178 (=HL 862 + pHL 282)pLtetO-1:mCherry, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1329 (=HL 865 + pHL 344)pLtetO-1:mCherry, pLlacO-1:rpoS::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1474 (=HL 862 + pHL 404)pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2300 (=HL 716 + pHL 745)pLtetO-1:RhyB-mCherry, pLlacO-1:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2304 (=HL 716 + pHL 269)pLtetO-1:MicC-mCherry, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2305 (=HL 716 + pHL 765)pLtetO-1:MicC, pLtetO-1:DsrA, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2307 (=HL 716 + pHL 777)pLtetO-1:MicC, pLtetO-1:RhyB, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2308 (=HL 716 + pHL 814)pLtetO-1:MicC, pLtetO-1:OxyS, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2312 (=HL 716 + pHL 787)pLtetO-1:MicC, pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2314 (=HL 716 + pHL 818)pLtetO-1:MicC, pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2625 (=HL 716 + pHL 908)pLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2627 (=HL 770 + pHL 908)pLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2729 (=HL 716 + rhyB knockout_KanR)Kanamycin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2817 (=HL 716 + pHL 720)pLtetO-1:mCherry, pLlacO-1:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2818 (=HL 865 + pHL 489)pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2839 (=HL 2752 + pHL 720)pLtetO-1:mCherry, pLlacO-1:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2869 (=HL 2752 + pHL 991)pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3076 (=HL 716 + pHL 1069)pCon:MicC, pLlacO-1:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3151 (=HL 716 + pHL 1037)pLtetO-1:DsrA, pCon:MicC, pLlacO-1:rpoS::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3152 (=HL 716 + pHL 1056)pCon:MicC, pLlacO-1:rpoS::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3186 (=HL 716 + pHL 282)pLtetO-1:mCherry, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3326 (=HL 716 + pHL 1008)pLlacO-1:DsrA-mCherry, pLtetO-1:RBS (st7) gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3327 (=HL 716 + pHL 1009)pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3330 (=HL 770 + pHL 1009)pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3325 (=HL 770 + rhyB knockout_KanR)Kanamycin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3347 (=HL 3338 + pHL 720)pLtetO-1:mCherry, pLlacO-1:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3357 (=HL 716 + pHL 1191)pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3375 (=HL 865 + pHL 1008)pLlacO-1:DsrA-mCherry, pLtetO-1:RBS (st7) gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3395 (=HL 862 + pHL 1009)pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3448 (=HL 3425 + pHL 1191)pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3449 (=HL 3262 + pHL 1179)pLtetO-1:mCherry, pLlacO-1:fhlA::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3450 (=HL 3262 + pHL 1191)pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3545 (=HL 770 + pHL 1213)pLlacO-1:hfq, pLtetO-1:RBS (st7) gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3612 (=HL 716 + pHL 841)pLtetO-1:RhyB-mCherry, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3619 (=HL 716 + pHL 1228)pLtetO-1:DsrA-mCherry, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3722 (=HL 716 + pHL 404)pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3723 (=HL 716 + pHL 489)pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3724 (=HL 716 + pHL 991)pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3858 (=HL 716 + pHL 1304)pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3859 (=HL 716 + pHL 1305)pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3904 (=HL 770 + pHL 991)pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4120 (=HL 716 + pHL 1370)pLtetO-1:DsrA, pCon:T7(st3) (900bps), pLlacO-1:rpoS::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4175 (=HL 716 + pHL 1391)pLlacO-1:rpoS::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4178 (=HL 862 + pHL 1360)pLlacO-1:hfq, pLtetO-1:ompC::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4179 (=HL 865 + pHL 1381)pLlacO-1:hfq, pLtetO-1:rpoS::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4180 (=HL 2752 + pHL 1358)pLlacO-1:hfq, pLtetO-1m9:sodB::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4202 (=HL 716 + pHL 1389)pLtetO-1:DsrA, pLlacO-1:rpoS::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4212 (=HL 716 + pHL 1414)pCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfpAmpicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4218 (=HL 716 + pHL 1411)pLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp (Other)Ampicillin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL13See commentsChloramphenicol Lim A multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75.
HL23See comments, See commentsChloramphenicol Lim A multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75.
HL24See commentsChloramphenicol Lim A multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75.
HL168See commentsChloramphenicol Lim A multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75.
HL3796See commentsChloramphenicol Lim Han Lim Lab Strains (unpublished)
HL4142See commentsChloramphenicol Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
HL5086See commentsKanamycin Lim Minimal effect of gene clustering on expression in Escherichia coli. Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5.
HL5113See commentsKanamycin Lim Minimal effect of gene clustering on expression in Escherichia coli. Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5.
HL3515See commentsKanamycin Lim Fundamental relationship between operon organization and gene expression. Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10626-31. doi: 10.1073/pnas.1105692108. Epub 2011 Jun 13.
HL3953See commentsKanamycin Lim Minimal effect of gene clustering on expression in Escherichia coli. Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5.
HL3546See commentsKanamycin Lim Fundamental relationship between operon organization and gene expression. Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10626-31. doi: 10.1073/pnas.1105692108. Epub 2011 Jun 13.
HL3573See commentsKanamycin Lim Minimal effect of gene clustering on expression in Escherichia coli. Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5.
HL3578See commentsKanamycin Lim Minimal effect of gene clustering on expression in Escherichia coli. Genetics. 2013 Feb;193(2):453-65. doi: 10.1534/genetics.112.147199. Epub 2012 Dec 5.
Strain Phi80 JQ929581mKate2 (Synthetic), Superfolder GFP (Synthetic)Kanamycin Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
Strain Phi80 JQ929582mKate2 (Synthetic), Superfolder GFP (Synthetic)Kanamycin Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
Strain HK022 JQ929581mKate2 (Synthetic), Superfolder GFP (Synthetic)Kanamycin Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
rEc2oriT-kan templateKanamycin Isaacs CAGE (unpublished)
rEc 27.H.ScspecR templateSpectinomycin Isaacs CAGE (unpublished)
pRK24broad-host range natural conjugative plasmidE. coli Conjugation genesTetracycline Isaacs CAGE (unpublished)
HL 713MG 1655 + lacIq_KanR inserted into the chromosome at the intS site none (Other)Kanamycin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 751MG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq_KanRnone (Other)Kanamycin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 852MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA_KanRnone (Other)Kanamycin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1120MG 1655 + lacIq inserted into the chromosome at the intS site + ΔmicC + Δhfq_KanRnone (Other)Kanamycin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1179MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA + Δhfq_KanRnone (Other)Kanamycin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3221MG 1655 + lacIq inserted into the chromosome at the intS site + ΔoxyS_KanRnone (Other)Kanamycin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3387MG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq + ΔoxyS_KanRnone (Other)Kanamycin Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1216MG 1655 + lacIq inserted into the chromosome at the intS site + ΔmicC + ΔaraC_KanRnone (Other)Kanamycin Lim Direct comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22.
HL 1254MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA + ΔaraC_KanRnone (Other)Kanamycin Lim Direct comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22.
HL 932MG1655 + ΔlacInone (Other)none Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
HL 1255MG1655 + fimS (off orientation)::gfp::Asp terminator::CamR at fimA none (Other)Chloramphenicol Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
HL 1285MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA none (Other)none Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
HL 4195MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(partial)none (Other)Kanamycin Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
HL 4233ΔfimB + ΔfimE + ΔfimA none (Other)none Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
HL 6139MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(stop codon)none (Other)Kanamycin Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
HL 6166MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(IRL)none (Other)Kanamycin Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
HL 6279MG1655 + fimS (off orientation)::gfpAAV tag::Asp terminator::CamR at fimA + ΔlacI + KanR replaces fimB and fimE(partial)none (Other)Kanamycin Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
HL3721HL716 + ΔcsrB + ΔcsrC + ΔglgCAP + ΔpgaABCDnone (Other)none Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
HL3796HL716 + ΔcsrA + ΔcsrB + ΔcsrC + ΔglgCAP + ΔpgaABCDnone (Other)none Lim Lim Lab unpublished plasmids (csrA crosstalk) (unpublished)
HL4018HL716 + ΔcsrB + ΔcsrC + ΔcsrD + ΔglgCAP + ΔpgaABCDnone (Other)none Lim Lim Lab unpublished plasmids (csrA crosstalk) (unpublished)
pSEVAT1Z1AGeneGuard vector cassette: thyA, R6K COR, Zeta toxin; with ampR as cargo.Ampicillin Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD1Z1AGeneGuard vector cassette: dapA, R6K COR, Zeta toxin; with ampR as cargo.Ampicillin Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAT1K1AGeneGuard vector cassette: thyA, R6K COR, Kid toxin; with ampR as cargo.Ampicillin Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD1K1AGeneGuard vector cassette: dapA, R6K COR, Kid toxin; with ampR as cargo.Ampicillin Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAT7Z1AGeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo.Ampicillin Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD7Z1AGeneGuard vector cassette: dapA, ColE2 COR, Zeta toxin; with ampR as cargo.Ampicillin Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAT7K1AGeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo.Ampicillin Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD7K1AGeneGuard vector cassette: dapA, ColE2 COR, Kid toxin; with ampR as cargo.Ampicillin Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
HL 716Lim Lab StrainSee commentsNone Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 770Lim Lab StrainSee commentsNone Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 862Lim Lab StrainSee commentsNone Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 865Lim Lab StrainSee commentsNone Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1128Lim Lab StrainSee commentsNone Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1188Lim Lab StrainSee commentsNone Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2752Lim Lab StrainSee commentsNone Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3338Lim Lab StrainSee commentsNone Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1745Lim Lab StrainSee commentsNone Lim Regulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24.
HL 1852Lim Lab StrainSee commentsNone Lim Regulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24.
HL 1951Lim Lab StrainSee commentsNone Lim Regulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24.
HL 2028Lim Lab StrainSee commentsNone Lim Regulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24.
HL 5220Lim Lab StrainSee commentsNone Lim Regulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24.
HL 5221Lim Lab StrainSee commentsNone Lim Regulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24.
HL 5222Lim Lab StrainSee commentsNone Lim Regulatory consequences of gene translocation in bacteria. Nucleic Acids Res. 2012 Oct;40(18):8979-92. doi: 10.1093/nar/gks694. Epub 2012 Jul 24.
E. coli K-12 MG1655 RAREThis E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydesNone Prather Synthesis and accumulation of aromatic aldehydes in an engineered strain of Escherichia coli. J Am Chem Soc. 2014 Aug 20;136(33):11644-54. doi: 10.1021/ja506664a. Epub 2014 Aug 8.
HL2483Lim Lab strain: MG1655 + lacIq intergrated at intS + Pcon::TetR inserted into the chromosome at galK + ΔglgCAP + ΔcsrB + ΔcsrC + ΔcsrDsee commentsNone Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
HL5410Lim Lab strain: MG1655 + lacIq intergrated at intS + ΔglmZ + Dhfqsee commentsNone Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
HL 5226Lim Lab strain: MG1655 + lacIq intergrated at intS + ΔglmZsee commentsNone Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
CY026Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlRRhlR, CinRChloramphenicol Bennett SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium. Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
CY027Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlRNone Bennett SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium. Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.

Addgene Blugene icon

Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!