...directly transfect cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning applications... analyses are performed on my cloning grade DNA order? For each cloning grade DNA prep, the plasmid sequence...like to see available as cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form... ordering cloning grade DNA for over 200 of our most popular plasmids! DNA... DNA Service: Cloning Grade DNA ...Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over 200 plasmids in our ...variety of popular plasmids and backbones with high cloning potential. By making these plasmids available as...

...related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems....Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning and Genomic Tools...Tools Synthetic Biology: Cloning and Genomic Tools SynBio Resources SynBio Home Browse All SynBio Assembly...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination...Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table by keyword or sort by...

...heavy joining group IGHJ IGHJ1 immunoglobulin heavy joining 1 JH1 IGHJ2 immunoglobulin heavy joining 2 JH2...heavy joining 3 JH3b IGHJ4 immunoglobulin heavy joining 4 JH4b IGHJ5 immunoglobulin heavy joining 5 JH5b...kappa joining group IGKJ IGKJ1 immunoglobulin kappa joining 1 J1 IGKJ2 immunoglobulin kappa joining 2 J2... kappa joining 3 J3 IGKJ4 immunoglobulin kappa joining 4 J4 IGKJ5 immunoglobulin kappa joining 5 J5 IGKV...lambda joining 2 J2 IGLJ3 immunoglobulin lambda joining 3 J3 IGLJ4 immunoglobulin lambda joining 4 (non-functional...- CHGA chromogranin A (parathyroid secretory protein 1) CGA CHGB chromogranin B (secretogranin 1) SCG1...ODG1 GAL galanin prepropeptide GALN, GLNN, GMAP, MGC40167 GALP galanin-like peptide - GALR2 galanin receptor...

...PI 31186 pEVOL-pAzF p-azidohenylalanine RS M. jannaschii p-azido-l-phenylalanine Bacterial TAG Peter Schultz...31190 pEVOL-pBpF p-benzoylphenylalanine RS M. jannaschii p-benzoyl-l-phenylalanine Bacterial TAG Peter...85494 pDule-pCNF para-cyanophenylalanine synthetase M. jannaschii azidoPhenylalanine Bacterial TAG Ryan ...85495 pDule2-pCNF para-cyanophenylalanine synthetase M. jannaschii azidoPhenylalanine Bacterial TAG Ryan ... non-canonical amino acids (ncAAs). A plasmid containing the gene of interest with the modified codon ...gene of interest can be expressed from a plasmid containing a UAG codon at the place where the new amino ...therapeutics to biopolymers. Tips for Success Before beginning to reprogram the genetic code, there are several...

...regions. Blue/white screening can be used for screening of transformants in cloning process. Lynne Postovit...with scientists since its release. Luciferase-containing plasmids are commonly used to investigate the...artificial luciferase desined de novo using deep-learning approaches. Split-Luciferase and Protein-Protein...plasmids : Luminescence-based protein-protein screening method for the systematic and quantitative analysis...Renilla and Firefly luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot...created by fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Nano-lanterns , Enhanced...utilizing Firefly luciferase. Transcriptional Element Screening Optimized STARR-seq ( S elf- T ranscribing A ctive...

...gRNA design and cloning gRNA cloning vector PDF 115.2 KB Church gRNA design and cloning for Cas9 orthologs...optimized Cas9 PDF 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo...Fly: gRNA cloning pAc-sgRNA-Cas9 PDF 165.1 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF ...Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF 125.9 KB Sontheimer gRNA design and cloning All-in-one...All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning in gRNA: pSimpleII-U6-...the Broad Institute gives practical advice for designing your gRNA and introducing it into cells. Protocol... the quantification of both non-homologous end joining (NHEJ) and homologous directed repair (HDR) frequency...

...Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...sequence. We have found that adding an additional guanine nucleotide to the 5’ of 20-bp guide can increase...RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries...Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator...database (23,430 isoforms) for gain-of-function screening. For more information, please visit the Addgene...activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...

.... P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (2015). An updated...in your lab, from knockouts to pooled library screening. Science...CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9 Alternatives Resources ...the efficient but error-prone non-homologous end joining (NHEJ) pathway and the less efficient but high-... , and increases the likelihood that any cell containing the CRISPR plasmid will have all desired genomic...researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single plasmid — though some...coding sequence ~1 kb shorter than SpCas9 while retaining the same basic function. Its size allows it to...

...Anti-Calretinin [L122/68R] Calretinin Human Mouse IgG2a 177469 Anti-Calretinin [L122/6R] Calretinin Human...220384 Calretinin [L122/110R] Calretinin Human Mouse IgG2a 220385 Calretinin [L122/117R] Calretinin Human...220386 Calretinin [L122/132R] Calretinin Human Mouse IgG2a 220387 Calretinin [L122/133R] Calretinin Human...220388 Calretinin [L122/136R] Calretinin Human Mouse IgG2a 220389 Calretinin [L122/20R] Calretinin Human...-CALB2/calretinin [L122/68R-2b] CALB2/calretinin Human Mouse IgG2b 206642 Anti-CALB2/calretinin [L122/...-CALB2/calretinin [L122/68R-1] CALB2/calretinin Human Mouse IgG2a 206691 Anti-CALB2/calretinin [L122/6R... receptor Rat Mouse IgG2a 114484 Anti-Stonin-2 [N346/9R] Stonin-2 Human Mouse IgG2a 114485 Anti-NSD3 [...

... for Cloning 67720 Worm NotI/BamHI none S. pyogenes Colaiacovo pRB17 52527 Fly blunt end cloning none ...yes, cut N. meningitidis Thomson pSmart-Nm-sgRNA-BbsI 49157 Mammalian U6 none N. meningitidis Thomson pXCas9H840A...none N. meningitidis Pederson pLH-stsgRNA1.1 64116 Mammalian/Lentiviral U6 none N. meningitidis Pederson...none N. meningitidis Pederson pLH-stsgRNA3.1 64118 Mammalian/Lentiviral U6 none N. meningitidis Pederson...the same cell at the same time. Single plasmids containing both the gRNA and a Cas protein act as an all-in-one...or nick. Selection , such as Puromycin or EGFP Cloning enzyme used for insertion of your gRNA sequence...used with, such as S. pyogenes, S. aureus, N. meningitidis, etc. gRNA scaffolds are specific for the species...

... in order to delete the intervening DNA segment by non-homologous end joining (NHEJ) repair. Deletions...present a simple methodology for CRISPR design, cloning, and delivery for the production of genomic deletions...complement including additional nucleotides for cloning and expression purposes. NOTE: Here we discuss ...AAAC” before the guide’s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme...be 24-mer oligos ( Table 4 ). Design Deletion Screening Primers Design one set of primers internal to ...primers described in step 2.1 may be omitted. CRISPR Cloning Anneal and phosphorylate oligos. Resuspend oligos...oligos into pX330 using a Golden Gate assembly cloning strategy 3 . Prepare a 50 μl reaction mix: 100 ...

... RALGDS Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange...antagonist STK STK3 STK4 Serine/threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase...small GTPase and is a member of the G protein (guanine nucleotide binding protein) family. GTPases serve...Rho GTPase activating protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3...binding protein Rac) RAF AFAF BRAF RAF1 Serine/threonine kinases: A-Raf proto-oncogene B-Raf proto-oncogene...like RASGRF RASGRF1 RASGRF2 Ras protein specific guanine nucleotide releasing factor RASGRP RASGRP1 RASGRP2...member ROCK ROCK1 ROCK2 Rho associated coiled-coil containing protein kinase ROS1 ROS proto-oncogene 1, receptor...

...resistances. Comprehensive multi-cloning site compatible with standard cloning using many different restriction.... pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged TALEN.... pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged TALEN...epitope tags like His for easy western blotting or staining, and RNA transcript tags to monitor RNA localization...His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose/IPTG inducible...formation or prevent toxicity. Compatible with Gateway cloning. pGTvL1-SGC 39188 pTac (lactose/IPTG inducible)... and TEV cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB...

...spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin Serotonin (5-HT) sensor...measuring serotonin dynamics. Nat Neurosci. 2021 Apr 5. Yulong Li , J. Julius Zhu Serotonin Serotonin sensor...19(2):231-241. Tommaso Patriarchi Serotonin Red and green serotonin sensors GRAB_5-HT3.0 Dual-color GRAB...Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning. Cell. 2020 Dec 12. pii: S0092-8674...-8674(20)31612-3. Lin Tian Serotonin Serotonin (5-HT) sensor psychLight Psychedelic-inspired drug discovery...: S0092-8674(21)00374-3. Lin Tian Serotonin Cilia-targeted serotonin sensor GRAB-HTR6 A serotonergic axon-cilium...calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck...

... Parniske. GreenGate Cloning System to create plant expression vectors containing several cassettes and...Scott Lenaghan. Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional...as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Return to...Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent...in plants. The tables below highlight plasmids spanning a wide variety of molecular biology technologies...proteins. Addgene’s collection includes plasmids containing promoters that drive general and species-specific...Vladimir Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized...

...should sequence the region you are planning to modify prior to designing your gRNA, as sequence variation... into plasmids containing the gRNA scaffold using standard restriction-ligation cloning. However, the ...amplification of targeted region from DNA, subcloning into a plasmid, and screening individual clones. PCR amplification...sequence. For example, gRNA targeting sequences containing a G nucleotide at position 20 (1 bp upstream ...of the PAM) may be more efficacious than gRNAs containing a C nucleotide at the same position in spite ...design software ). Additionally, many plasmids containing validated gRNAs are now available through Addgene...the exact cloning strategy will depend on the gRNA vector you have chosen, so it is best to review the protocol...

...Adenoviral For recombining shuttle plasmids Vogelstein 16401 pAdEasy-2 Adenoviral For recombining shuttle plasmids...16402 pShuttle Shuttle For production of viruses containing transgene under a chosen promoter Vogelstein ...pShuttle-CMV Shuttle For production of viruses containing transgene under CMV promoter Vogelstein 16404...Shuttle For production of GFP-trackable viruses containing transgene under a chosen promoter Vogelstein ...Shuttle For production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407...Shuttle For production of mRFP-trackable viruses containing transgene under CMV Bamburg 50958 ShuttleNSE ...ShuttleNSE Shuttle For production of viruses containing transgene under Neuron-specific enolase (NSE) promoter ...

...46910 pHR-SFFV-dCas9-BFP Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS ...pHR-SFFV-dCas9-BFP-KRAB Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS,...pMSCV-LTR-dCas9-VP64-BFP Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x ...51023 pSLQ1658-dCas9-EGFP Human expression vector containing dCas9 that is fused to 2x NLS and EGFP for CRISPR...pMSCV-LTR-dCas9-p65AD-BFP Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x ...46914 pU6-sgGFP-NT1 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1...46917 pU6-sgCXCR4-2 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous...

...Anthony Brown 11908 pEGFP-N1 alpha-actinin 1 Actin Filaments alpha-actinin 1 EGFP Carol Otey 27676 Cortactin-pmCherryC1... Protein PI 71367 Human Beta-catenin GFP Adherens Junctions Beta-catenin EGFP Alpha Yap 67937 mouse E-...Huttenlocher 11908 pEGFP-N1 alpha-actinin 1 Focal Adhesions alpha-actinin 1 EGFP Carol Otey 31923 pPaxillin-PSmOrange...Michael Davidson 55001 mCherry-Beta-Catenin-20 Adherens Junctions Beta-Catenin mCherry Michael Davidson 30313... resides. There are a variety of methods for determining subcellular localization. One of the most common...guide to find the appropriate backbone). Then, by examining the overlap between your fusion protein and the...the cell’s internal framework that assists in sustaining structure and function. The cytoskeleton is primarily...

...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance...preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions... repaired using error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted...your gene of interest’s promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express... same cell at the same time. Single plasmids containing both the gRNA and Cas protein act as all-in-one...