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We narrowed to 2,009 results for: D10

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Showing: 1981 - 2000 of 2009 results

  1. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

    Plasmid
    #129046
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA6 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129046-plasmid-map-sequence-id-256220

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  2. L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid
    #244178
    Purpose
    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human TNFR1 SS, ECD (no MMP site) and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1A Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    TNFR1 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244178-plasmid-map-sequence-id-487910

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  3. L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2

    Plasmid
    #244177
    Purpose
    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1B Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    TNFR2 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244177-plasmid-map-sequence-id-487904

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  4. pCMV-T7-TadCBEd-SpCas9(MRKQKC)-P2A-EGFP (AHK574)

    Plasmid
    #223134
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(MRKQKC)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/MRKQKC)=D1135M, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223134-plasmid-map-sequence-id-447700

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  5. pCMV-T7-TadCBEd-SpCas9(MRKSKC)-P2A-EGFP (AHK446)

    Plasmid
    #223135
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(MRKSKC)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/MRKSKC)=D1135M, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223135-plasmid-map-sequence-id-447701

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  6. pCMV-T7-TadCBEd-SpCas9(KWRQLC)-P2A-EGFP (BKS1026)

    Plasmid
    #223126
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(KWRQLC)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/KWRQLC)=D1135K, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223126-plasmid-map-sequence-id-447694

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  7. pCMV-T7-TadCBEd-SpCas9(LWRYEK)-P2A-EGFP (BKS1022)

    Plasmid
    #223128
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(LWRYEK)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWRYEK)=D1135L, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223128-plasmid-map-sequence-id-447696

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  8. pCMV-T7-TadCBEd-SpCas9(MKRCMV)-P2A-EGFP (BKS1032)

    Plasmid
    #223129
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(MKRCMV)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/MKRCMV)=D1135M, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223129-plasmid-map-sequence-id-447698

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  9. pCMV-T7-TadCBEd-SpCas9(MKRCKS)-P2A-EGFP (RAS3445)

    Plasmid
    #223130
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(MKRCKS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/MKRCKS)=D1135M, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223130-plasmid-map-sequence-id-447697

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  10. pCMV-T7-TadCBEd-SpCas9(ARGIMR)-P2A-EGFP (BKS1036)

    Plasmid
    #223132
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(ARGIMR)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/ARGIMR)=D1135A, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223132-plasmid-map-sequence-id-451166

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  11. pCMV-T7-TadCBEd-SpCas9(MRKCRS)-P2A-EGFP (AHK545)

    Plasmid
    #223133
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(MRKCRS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/MRKCRS)=D1135M, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223133-plasmid-map-sequence-id-447699

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  12. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid
    #222508
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; C706A and R832…
    Promoter
    TRE3GV and hPGK
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222508-plasmid-map-sequence-id-441225

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  13. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61361-plasmid-map-sequence-id-99794

    Enlarge

  14. L4341 TNFR1 NTEVp chain (deleted MMP site) in PolyTX-mNeonGreen

    Plasmid
    #244176
    Purpose
    Constitutive expression of a MESA NTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-NTEVp(75S)
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA NTEVp chain with human TNFR1 SS and ECD (no MMP site), murine CD28 TMD, and NTEVp 75S mutant (TNFRSF1A Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    TNFR1 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244176-plasmid-map-sequence-id-487905

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  15. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

    Plasmid
    #192704
    Purpose
    Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPm…
    Expression
    Mammalian
    Mutation
    meGFPm: gluatmic acid 142 to asparagine and histi…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192704-plasmid-map-sequence-id-379659

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  16. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

    Enlarge

  17. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid
    #192784
    Purpose
    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192784-plasmid-map-sequence-id-379372

    Enlarge

  18. pLY162

    Plasmid
    #184138
    Purpose
    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-R1, PrhaB, and Ptet
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184138-plasmid-map-sequence-id-376257

    Enlarge

  19. pLY163

    Plasmid
    #184141
    Purpose
    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-R2, PrhaB, and Ptet
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184141-plasmid-map-sequence-id-376139

    Enlarge

  20. pLY315

    Plasmid
    #184160
    Purpose
    Reporter circuit for hijacking mRNA of zntA as CRISPR RNA
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-zntA-1034, PrhaB, and Ptet
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    184160-plasmid-map-sequence-id-376259

    Enlarge

Showing: 1981 - 2000 of 2009 results