We narrowed to 18 results for: D10

....13, Hu et al. first restored catalytic residues D10 and H840. To ensure that the clones were capable ...

...Reagent Preparation DMEM Complete (also known as D10): 10% v/v FBS and 4 mM L-alanyl-L-glutamine (or stable...

...spacing D10A: nick sites separated by 37-68 bp H840A: nick sites separated by 51-68 bp Use D10A for HDR...use SpCas9 nickases as examples for this post. The D10A mutation inactivates the RuvC domain, so this nickase...preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in and...higher in the PAM-out configuration. In addition, D10A editing efficiency is consistently higher than that...H840A, especially with smaller nick distances. Using D10A, they subsequently showed that editing efficiency...gRNAs are too close (7-23 nt nick distance). Both D10A and H840A are potent editors, but their indel profiles...profiles vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased...

...instead of Cas9, this plasmid expresses Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA....

... deletions (indel) by the catalytically impaired D10A nickase from ABE7.10 can be a problem. Therefore...

...enzymatic residues has been converted to an alanine (D10A or H840A), changing its double-strand cut to a single-stranded...

... other than NGG (Table-1). Type Nickase Cas9 (D10A) Deaminase PAM requirements Reference CBE SpCas9...

...expressed them along with Cas9 in the T-cell line 2D10. PCR amplification and Sanger sequencing of pooled...

... Cas9 variants are available: wt humanized Cas9, D10A nickase mutant (Cas9n), dCas9 (transcriptional repression...

.... The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong et al....

...PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase) and single...Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase) + single guide RNA: These plasmids contain...-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid...single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165; SpCas9...

...-ATPase, 1 MGC133040, MGC133041, P112, Rpn2, S1 PSMD10 proteasome (prosome, macropain) 26S subunit, non-ATPase...MGC142065, MGC142067 UBXN1 UBX domain protein 1 2B28, UBXD10 ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 ...) and short cytoplasmic domain, (semaphorin) 4A CORD10, FLJ12287, RP35, SEMAB, SEMB SEMA4B sema domain...short cytoplasmic domain, (semaphorin) 4D C9orf164, CD100, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-...membrane anchor (John Milton Hagen blood group) CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, MGC126692, MGC126696...avian) ERBB, ERBB1, HER1, PIG61, mENA ENG endoglin CD105, END, FLJ41744, HHT1, ORW, ORW1 EPGN epithelial ...

...array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF, 255 KB Zhang...marker); pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid...

...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with... 2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector ...

...rProtein A GraviTrap columns, Cytiva 28-9852-54 LabMate PD10 Buffer Reservoir, Millipre Sigma GE18-3216-03 5%...the ethanol storage buffer. Tightly attach LabMate PD10 Buffer Reservoirs to the top of the Gravitrap column...

...single cut (nick) or no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain...dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in no cleavage of target DNA...

...Halo_C_allele PFN1 Halo ALS Michael Ward 178118 CHCHD10_Halo_N_allele CHCHD10 Halo ALS Michael Ward 178119 UBQLN2_... SOD1 His T7 ALS Elizabeth Fisher 117704 phSOD1-D101G-N'His SOD1 His T7 ALS Elizabeth Fisher 117705 pmSod1...tac Parkinson's Kalle Gehring 227362 pGEX-parkin D106A PRKN GST, HRV 3C tac Parkinson's Kalle Gehring 227363...

...single cut (nick) or no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain...dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in no cleavage of target DNA...