We narrowed to 20 results for: D10

1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   ....13, Hu et al. first restored catalytic residues D10 and H840. To ensure that the clones were capable ...

2. Lentivirus Production

   Type

   Protocol

   ...Reagent Preparation DMEM Complete (also known as D10): 10% v/v FBS and 4 mM L-alanyl-L-glutamine (or stable...

3. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   ...spacing D10A: nick sites separated by 37-68 bp H840A: nick sites separated by 51-68 bp Use D10A for HDR...use SpCas9 nickases as examples for this post. The D10A mutation inactivates the RuvC domain, so this nickase...preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in and...higher in the PAM-out configuration. In addition, D10A editing efficiency is consistently higher than that...H840A, especially with smaller nick distances. Using D10A, they subsequently showed that editing efficiency...gRNAs are too close (7-23 nt nick distance). Both D10A and H840A are potent editors, but their indel profiles...profiles vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased...

4. CRISPR/Cas9 FAQs Answered!

   Type

   Blog Post

   .... The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al...

5. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   ...instead of Cas9, this plasmid expresses Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA....

6. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   ... deletions (indel) by the catalytically impaired D10A nickase from ABE7.10 can be a problem. Therefore...

7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   ...enzymatic residues has been converted to an alanine (D10A or H840A), changing its double-strand cut to a single-stranded...

8. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   ... other than NGG (Table-1). Type Nickase Cas9 (D10A) Deaminase PAM requirements Reference CBE SpCas9...

9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   ...expressed them along with Cas9 in the T-cell line 2D10. PCR amplification and Sanger sequencing of pooled...

10. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post

    ... Cas9 variants are available: wt humanized Cas9, D10A nickase mutant (Cas9n), dCas9 (transcriptional repression...

11. Zhang Lab CRISPR Page

    Type

    Collection

    ...PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase) and single...Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase) + single guide RNA: These plasmids contain...-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid...single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165; SpCas9...

12. Immunology Research Plasmids and Resources

    Type

    Collection

    ...-ATPase, 1 MGC133040, MGC133041, P112, Rpn2, S1 PSMD10 proteasome (prosome, macropain) 26S subunit, non-ATPase...MGC142065, MGC142067 UBXN1 UBX domain protein 1 2B28, UBXD10 ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 ...) and short cytoplasmic domain, (semaphorin) 4A CORD10, FLJ12287, RP35, SEMAB, SEMB SEMA4B sema domain...short cytoplasmic domain, (semaphorin) 4D C9orf164, CD100, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-...membrane anchor (John Milton Hagen blood group) CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, MGC126692, MGC126696...avian) ERBB, ERBB1, HER1, PIG61, mENA ENG endoglin CD105, END, FLJ41744, HHT1, ORW, ORW1 EPGN epithelial ...

13. CRISPR References and Information

    Type

    Collection

    ...array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF, 255 KB Zhang...marker); pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid...

14. Lentiviral Prep Service

    Type

    Collection

    ...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with... 2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector ...

15. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ...rProtein A GraviTrap columns, Cytiva 28-9852-54 LabMate PD10 Buffer Reservoir, Millipre Sigma GE18-3216-03 5%...the ethanol storage buffer. Tightly attach LabMate PD10 Buffer Reservoirs to the top of the Gravitrap column...

16. CRISPR Guide

    Type

    Collection

    ...single cut (nick) or no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain...dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in no cleavage of target DNA...

17. Zhang Lab's CRISPR Frequently Asked Questions

    Type

    Collection

    .... The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al...

18. Neurodegeneration Plasmid Collection

    Type

    Collection

    ...Halo_C_allele PFN1 Halo ALS Michael Ward 178118 CHCHD10_Halo_N_allele CHCHD10 Halo ALS Michael Ward 178119 UBQLN2_... SOD1 His T7 ALS Elizabeth Fisher 117704 phSOD1-D101G-N'His SOD1 His T7 ALS Elizabeth Fisher 117705 pmSod1...

19. CRISPR Guide

    Type

    Guide

    ...single cut (nick) or no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain...dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in no cleavage of target DNA...

20. Cre-lox system

    Type

    Collection

    ... transcription of Cre lac Bacterial Ekker 47551 pDD104 (Peft-3::Cre) Cre eft-3 C. elegans Goldstein 48201...