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Showing: 1 - 20 of 24 results
  1. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...spacing D10A: nick sites separated by 37-68 bp H840A: nick sites separated by 51-68 bp Use D10A for HDR...use SpCas9 nickases as examples for this post. The D10A mutation inactivates the RuvC domain, so this nickase...preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in and...higher in the PAM-out configuration. In addition, D10A editing efficiency is consistently higher than that...H840A, especially with smaller nick distances. Using D10A, they subsequently showed that editing efficiency...gRNAs are too close (7-23 nt nick distance). Both D10A and H840A are potent editors, but their indel profiles...profiles vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased...
  2. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    .... The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al...
  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase) and single...Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase) + single guide RNA: These plasmids contain...-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid...single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165; SpCas9...
  4. Gersbach Lab CRISPR Plasmids

    Type
    Collection
    ...expression plasmids encode the nuclease-null dCas9 (D10A, H840A) with HA and FLAG epitope tags either alone...pcDNA-dCas9 : Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells. 47107 pcDNA-dCas9-VP64...VP64 : Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian...
  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ...-ATPase, 1 MGC133040, MGC133041, P112, Rpn2, S1 PSMD10 proteasome (prosome, macropain) 26S subunit, non-ATPase...MGC142065, MGC142067 UBXN1 UBX domain protein 1 2B28, UBXD10 ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 ...) and short cytoplasmic domain, (semaphorin) 4A CORD10, FLJ12287, RP35, SEMAB, SEMB SEMA4B sema domain...short cytoplasmic domain, (semaphorin) 4D C9orf164, CD100, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-...membrane anchor (John Milton Hagen blood group) CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, MGC126692, MGC126696...avian) ERBB, ERBB1, HER1, PIG61, mENA ENG endoglin CD105, END, FLJ41744, HHT1, ORW, ORW1 EPGN epithelial ...
  6. CRISPR References and Information

    Type
    Collection
    ...array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) ; PDF 248.6 KB Zhang...marker); pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF 102.5 KB Jaenisch CRISPR ...
  7. Doudna Lab CRISPR Plasmids

    Type
    Collection
    ...Cas9. 39315 pMJ825 : Streptococcus pyogenes Cas9 D10A mutation. 39316 pMJ826 : Streptococcus pyogenes ...Cas9. 39318 pMJ841 : Streptococcus pyogenes Cas9 D10A, H840A. 41091 pHMGWA-Pa14Csy4 : Pseudomonas aeruginosa...
  8. EXPRESs plasmids for human platelets

    Type
    Collection
    ...bio-His 51831 TM9-bio-His 51832 TMED1-bio-His 51852 TMED10-bio-His 51853 TMED2-bio-His 51854 TMED4-bio-His...bio-His 51860 PEAR1-bio-His 51861 LAMP2-bio-His 51862 CD109-bio-His 51863 F2R-bio-His 51864 F2RL3-bio-His 51865...
  9. Lentiviral Prep Service

    Type
    Collection
    ...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with... 2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector ...
  10. Cheng Lab CRISPR Casilio System

    Type
    Collection
    ..., use a nuclease-deficient S. pyogenes Cas9 with D10A and H840A mutations (dCas9). Addgene has many dCas9...
  11. Church Lab CRISPR Plasmids

    Type
    Collection
    ...codon-optimized Cas9 expression plasmid 41816 hCas9 D10A A human codon-optimized Cas9 expression plasmid ...
  12. CRISPR Guide

    Type
    Guide
    ... nuclease domains, RuvC and HNH. Cas9 nickase, a D10A mutant of SpCas9, retains one nuclease domain and...domains can be rendered inactive by point mutations (D10A and H840A in SpCas9), resulting in a nuclease dead...
Showing: 1 - 20 of 24 results