We narrowed to 9 results for: D10

1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ....13, Hu et al. first restored catalytic residues D10 and H840. To ensure that the clones were capable ...

2. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ...spacing D10A: nick sites separated by 37-68 bp H840A: nick sites separated by 51-68 bp Use D10A for HDR...use SpCas9 nickases as examples for this post. The D10A mutation inactivates the RuvC domain, so this nickase...preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in and...higher in the PAM-out configuration. In addition, D10A editing efficiency is consistently higher than that...H840A, especially with smaller nick distances. Using D10A, they subsequently showed that editing efficiency...gRNAs are too close (7-23 nt nick distance). Both D10A and H840A are potent editors, but their indel profiles...profiles vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased...

3. CRISPR/Cas9 FAQs Answered!

   Type

   Blog Post

   Published

   March 13, 2014, 4:08 p.m.

   .... The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al...

4. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...instead of Cas9, this plasmid expresses Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA....

5. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   Published

   May 7, 2020, 1:15 p.m.

   ... deletions (indel) by the catalytically impaired D10A nickase from ABE7.10 can be a problem. Therefore...

6. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ... other than NGG (Table-1). Type Nickase Cas9 (D10A) Deaminase PAM requirements Reference CBE SpCas9...

7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...enzymatic residues has been converted to an alanine (D10A or H840A), changing its double-strand cut to a single-stranded...

8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ...expressed them along with Cas9 in the T-cell line 2D10. PCR amplification and Sanger sequencing of pooled...

9. CRISPR 101: Multiplex Expression of gRNAs

   Type

   Blog Post

   Published

   Sept. 10, 2020, 11:45 a.m.

   ... Cas9 variants are available: wt humanized Cas9, D10A nickase mutant (Cas9n), dCas9 (transcriptional repression...