We narrowed to 4,232 results for: pCMV

1. pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

   Plasmid

   #163554

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-Anc689-UdgX-NG-nCas9-RBMX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

   Plasmid

   #134332

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA16_Lmo
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)

   Plasmid

   #134340

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIC1
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6-XL4 ASXL1 (1-479) 3x FLAG

   Plasmid

   #74262

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains ASXL1 amino acids 1-479

   Promoter

   5’LTR

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-FLEx-TetOn3G-3xHA-FLEx

   Plasmid

   #133729

   Purpose

   Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   TetOn3G-3xHA (floxed & inverted)
   

   Use

   AAV

   Tags

   3xHA-tag

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-SlugCBE-NNG-P2A-puro

   Plasmid

   #214355

   Purpose

   Expresses SlugCBE-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCBE-NNG and puromycin resistance genes
   

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV:: NES-FKBP-iLID-LRP6c

   Plasmid

   #221774

   Purpose

   Expresses NES-FKBP-iLID-LRP6c component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

   Insert

   pCMV:: NES-FKBP-iLID-LRP6c
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCMV-intron myc Rab6 T27N

   Plasmid

   #46782

   Purpose

   expression of T27N Rab6a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   Rab6 T27N (RAB6A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   T27N dominant negative

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-BiP-Myc-KDEL P495L

   Plasmid

   #27166

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Mutation

   Peptide Binding Mutant: P495L

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

    Plasmid

    #169586

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-SNAP-CRY2-VHH(GFP)

    Plasmid

    #58370

    Purpose

    For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Tags

    SNAP and vhhGFP4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-BiP-Myc-KDEL T37G

    Plasmid

    #27165

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    ATP bound form: T37G

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-E1347A-nCas9(D10A)-UGI

    Plasmid

    #187410

    Purpose

    DddA E1347A variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA E1347A variant
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid

    #174137

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-AAAAA-nCas9(D10A)-UGI

    Plasmid

    #187409

    Purpose

    DddA AAAAA variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA AAAAA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV HA hRB delta CDK + T373

    Plasmid

    #58912

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T373 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-MCS-eDHFR(69K6)-EGFP

    Plasmid

    #172101

    Purpose

    Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    22aa-eDHFR(69K6)-EGFP
    

    Expression

    Mammalian

    Mutation

    eDHFR(69K6): Hexalysine (K6) sequence is inserted…

    Promoter

    CMV

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCMV-intron myc Rab11 S25N

    Plasmid

    #46786

    Purpose

    expression of S25N Rab11a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB11A S25N (RAB11A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S25N dominant negative

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCMV 3xFLAG-LC3B Q116P G120

    Plasmid

    #129289

    Purpose

    Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid

    #160140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(nucleoplasmin)-3xHA-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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