We narrowed to 18 results for: pCMV
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TypeBlog Post.... Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab.... It can be used with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral..., this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral...
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Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope protein for producing...Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...cytosine base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already... -
Pooled Library Amplifications
TypeBlog Post... Protoc. 2017 with modifications Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled...1866 bp, plus a 20 bp barcode 400 ng Solid pCMV-Rep 78/68 SSM Library Amplification and Sequencing... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog Post...CMV-mito-GEM-GECO1 and blue flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget... -
Cre-lox system
TypeCollection...Expression System PI 8394 p209 pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green...Lentiviral Trono 12493 p259 pCMV-CRE-M (BglII) CREM CMV Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused...Simpson 106368 pCMV-Tag2B-NCre N-terminal split-Cre CMV Mammalian Hirrlinger 106369 pCMV-Tag3B-CCre C-terminal...Hirrlinger 106370 pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian Hirrlinger 106371 pCMV-Tag3B-ERT2CCre... Green 8401 p224 pCMV-RFP/CREM floxed RFP within CREM CMV Mammalian Green 8403 p153 pPr-cre Cre probasin...ligand binding domain CMV Mammalian Green 12496 p270 pCMV-CRE-M-AR(LNCaP) CREM fused to mutant AR ligand binding... binding domain CMV Mammalian Green 12497 p274 pCMV-CRE-M-AR(T) CREM fused to mutant AR ligand binding... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...158008 pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre 158009 pCMV-mGold-Actin-C...158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin mGold Francois St-Pierre 158002 pCMV-mGold-Rab4a-C...GFP Mitochondria ABCb10 GFP Orian Shirihai 158003 pCMV-mGold-CAF1-C-10 Nucleus CAF-1 mGold Francois St-...Mitochondria-Outer Membrane Mff acGFP1 Gia Voeltz 158007 pCMV-mGold-Mito-N-7 Mitochondria Cox8A mGold Francois...anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N-10 Golgi apparatus MannII mGold Francois... Late endosomes RAB7 DsRed Richard Pagano 158006 pCMV-mGold-Lysosome-N-20 Lysosome Lamp1 mGold Francois... -
Lentivirus Plasmids
TypeCollection...plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd...and 3rd generation transfer plasmids Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev,... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab...Note: pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For... -
Genetic Code Expansion
TypeCollection...164080 pCMV-MtPylRS (human-opti) PylRS M. thermophila acetyl-lysine Mammalian Tao Liu 164081 pCMV-MfPylRS...164195 pCMV-MtAcKRS (human-opti) AcKRS M. thermophila acetyl-lysine Mammalian Tao Liu 164196 pCMV-MfBulKRS...SepRS9 E. coli Sep Bacterial TAG Jesse Rinehart 71403 pCMV-DnpK PylRS M. barkeri N6‐(2‐(2,4‐dinitrophenyl)acetyl..., DiZSeK, or DiZHSeC Bacterial TAG P. Chen 91706 pCMV-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers...azidonorleucine (Anl) Mammalian Elizabeth Kirby 223508 pCMV_NESPylRS(AF)_hU6tRNAPyl PylRS (Y306A, Y384F) M. mazei...mazei Mammalian TAG Edward Lemke 223509 pCMV_NESPylRS(WT)_hU6tRNAPyl PylRS M. mazei Mammalian TAG Edward ... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post.... Grant Challen’s lab also created constitutive (pCMV-dCas9-D3A) and Tet-dependent (TetO-dCas9-D3A) constructs... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly... -
Retrovirus Plasmids
TypeCollection...pBS-CMV-gagpol Packaging MoMLV gag and pol Salmon 8454 pCMV-VSV-G Envelope Envelope protein; use with packaging... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...MbPyl pyrrolysyl-tRNA synthetase Mammalian Cells pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP... -
Luciferase Plasmid Collection
TypeCollection...target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase...of Venus-firefly luciferase Roland Friedel 170575 pCMV-FLuc Firefly CMV Retroviral expression of firefly... -
15 Hot Plasmids from 2017
TypeBlog Post... activity; protein purification vector 87439 pCMV-HF-BE3 HF-Cas9 (NGG) Very low off-target activity... -
Neurodegeneration Plasmid Collection
TypeCollection...Ding 186012 pCMV Flag-LRRK2 K17A/K18A LRRK2 Flag CMV Parkinson's Suzanne Pfeffer 186013 pCMV Flag-LRRK2...Cheryl Arrowsmith 213544 pCMV-mKO2-Parkin PRKN mKO2 CMV Parkinson's Lani Wu 213545 pCMV-Parkin(C431N)-A92mKO2...Parkinson's Lani Wu 213546 pCMV-Parkin(E16A) PRKN CMV Parkinson's Lani Wu 213547 pCMV-Parkin PRKN CMV Parkinson's...Spinocerebellar ataxia, infantile onset Smita Patel 205706 pCMV-alphaS-2 SNCA CMV Parkinson's Randy Schekman 205713...Parkinson's Lani Wu 213548 pCMV-Parkin-A92mKO2 PRKN mKO2 CMV Parkinson's Lani Wu 213549 pLv-CMV(trunc)-Parkin-A92mKO2...Dong-Er Zhang 13313 pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1...Jens Schwamborn 87085 pCMV4-ApoE2 APOE CMV Alzheimer's Bradley Hyman 87086 pCMV4-ApoE3 APOE CMV Alzheimer's... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...then resulted in the development of a new plasmid, pCMV14-3X-Flag-SARS-CoV-2 S, which is now easily available...