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pUAS-luc2

Plasmid

#24343

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

Luciferase

Tags

5XGAL4

Expression

Mammalian

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIalpha

Plasmid

#21226

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIalpha (Camk2a Rat)

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0001.

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Rheb

Plasmid

#19996

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Ras homolog enriched in brain 1 (RHEB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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p50 cFlag pcDNA3

Plasmid

#20018

Depositor

Stephen Smale

Insert

Nfkb1 (Nfkb1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Truncated at the p105 ligation site. It codes for…

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pMXs-hNANOG (Plath)

Plasmid

#18115

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

Nanog homeobox (NANOG Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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FCK-ChR2-GFP

Plasmid

#15814

Purpose

Lentiviral expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pET15b PKA Cat

Plasmid

#14921

Depositor

Susan Taylor

Article

Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1(apf)

Plasmid

#13846

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(apf) (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif Thr(183)-Pro-T…

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pMT3 p38

Plasmid

#12658

Depositor

John Kyriakis

Insert

p38alpha (MAPK14 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-HsPeg10rc3

Plasmid

#174859

Purpose

Expresses recoded human Peg10 in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

recoded Homo sapiens PEG10 (PEG10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-TAQ_E602D

Plasmid

#166944

Purpose

Bacterial expression of Taq DNA polymerase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

Thermus aquaticus DNA polymerase

Tags

6X His

Expression

Bacterial

Mutation

E602D

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHluorin

Plasmid

#130901

Purpose

Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHluorin (CD63 Human, Aequorea victoria)

Tags

pHluorin

Expression

Mammalian

Mutation

pHluorin inserted between Gln-36 and Leu-37 in ex…

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-Flp

Plasmid

#122969

Purpose

Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

Depositor

Lydia Freddolino

Article

Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

Insert

Flp recombinase

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pX330-BbsI-PITCh

Plasmid

#127875

Purpose

For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-KRAB-MeCP2

Plasmid

#122267

Purpose

SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Tags

dCas9-KRAB-MeCP2

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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BASU RaPID plasmid

Plasmid

#107250

Purpose

Expresses the BASU RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

BASU

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Puro

Plasmid

#79049

Purpose

Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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mRFP-Lact-C2

Plasmid

#74061

Purpose

Fluorescent biosensor for phosphatidylserine.

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 (MFGE8 Bovine)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK-IDH1-FLAG

Plasmid

#66802

Purpose

Tet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vector

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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MS2_GFP

Plasmid

#61764

Purpose

Expresses MS2 coat protein fused to GFP

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

MS2 coat protein fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

Ubc promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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