165,744 results

1. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

   Plasmid

   #101209

   Purpose

   Bacterial expression plasmid for SpCas9-HF1 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N497A/R661A/Q695A/Q926A
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_RAD51_Rad51

   Plasmid

   #110031

   Purpose

   Protein expression and purification of RAD51_Rad51

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   RAD51_Rad51 (RAD51 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB_tetO7_sfGFP

   Plasmid

   #196616

   Purpose

   Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   Superfolder GFP
   

   Expression

   Yeast

   Promoter

   tetO7 promoter

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. aMHC-mCherry-Rex-Blasticidin

   Plasmid

   #21228

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   mCherry

   Available Since

   June 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTipi2.1

   Plasmid

   #225877

   Purpose

   Allows for expression of recombinant antibodies

   Depositor

   Institute for Protein Innovation (IPI)

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. UbG76V-eGFP-V5His_20aa

   Plasmid

   #23970

   Depositor

   Nico Dantuma

   Article

   Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

   Insert

   Ub-G76V-eGFP-V5HIS_20aa
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   G76V mutation in Ubiquitin. Ub fused to N-termin…

   Available Since

   March 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyCatcher003-sfGFP

   Plasmid

   #133449

   Purpose

   Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003-sfGFP
   

   Tags

   His6 and TEV site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-U6-crRNA-empty

   Plasmid

   #134921

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   crRNA for CRISPR-Cas3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHEN2-SG4

   Plasmid

   #196071

   Purpose

   Express wild type SG4 in E.coli

   Depositor

   Shankar Balasubramanian

   Article

   Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

   Insert

   SG4
   

   Tags

   3XFLAG and 6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFPc3 K-RAS 4B

    Plasmid

    #236452

    Purpose

    transient overexpression of K-RAS 4B in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

    Insert

    K-RAS 4B (KRAS Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-SRGAP2A-EGFP

    Plasmid

    #168501

    Purpose

    Expression of full length SRGAP2 with a C-termonal EGFP fusion

    Depositor

    Franck Polleux

    Article

    Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

    Insert

    SRGAP2A (SRGAP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG1b

    Plasmid

    #80612

    Purpose

    pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Brachyury-eGFP

    Plasmid

    #21063

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Brachyury promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_DNASE2_WT

    Plasmid

    #81815

    Purpose

    Gateway Donor vector containing DNASE2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    DNASE2 (DNASE2 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc-C Med12His

    Plasmid

    #49240

    Purpose

    expresses human Med12 with His tag in insect cells

    Depositor

    Dylan Taatjes

    Article

    Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

    Insert

    Med12 (MED12 Human)
    

    Tags

    His

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo

    Plasmid

    #239948

    Purpose

    Expression of MFSD6 loop 3 tagged with rabbit IgG Fc2

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    Fc2-MFSD6(L3)
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFYF1548 EMX1

    Plasmid

    #47508

    Purpose

    human gRNA expression vector targeting EMX1

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EMX1 (EMX1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT120b

    Plasmid

    #122604

    Purpose

    BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    gIII, luxAB, guide RNA
    

    Use

    Synthetic Biology

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-HA-GBF1

    Plasmid

    #206415

    Purpose

    Mammalian expression of hGBF1

    Depositor

    Kazuhisa Nakayama

    Article

    Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

    Insert

    GBF1 (GBF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHLUK

    Plasmid

    #64181

    Purpose

    harbours marker genes HIS3, LEU2, URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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