Addgene

Showing: 2041 - 2060 of 157643 results

Results per page:

Sort results by:

mCherry-GAL9

Plasmid

#166689

Purpose

For creating a stable mCherry-GAL9 cell line

Depositor

Alan Sabirsh

Article

Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

Insert

LGALS9 (LGALS9 Human)

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFlag-pA-Tn5-Fl

Plasmid

#124601

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Steven Henikoff

Article

Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

Insert

Protein A and hyperactive Tn5 transposase (Tnp) fusion protein

Use

Tags

Protein A and Tn5 transposase fusion protein with…

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Lifeact-7

Plasmid

#54491

Purpose

Localization: Action, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mCherry-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
paavCAG-pre-mGRASP-mCerulean

Plasmid

#34910

Purpose

pre

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD_GFP11_T7LysH17A

Plasmid

#59591

Purpose

Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

Depositor

Richard Kingston

Article

Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

Type

Empty backbone

Use

Tags

6xHis-tag and GFP11

Expression

Bacterial

Mutation

Promoter

pARA

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
peGFP C3-SPG20

Plasmid

#218578

Purpose

Expresses GFP-tagged human SPG20 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458-mcherry

Plasmid

#161974

Purpose

Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Cas9-t2a-mcherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CuO-V5 CASC3 siRes1+2 f.l. WT

Plasmid

#158540

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Blasticidin

Plasmid

#75085

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA Blasto

Plasmid

#167904

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIX403-hNOS2

Plasmid

#110800

Purpose

tet-inducible NOS2 expression

Depositor

Edward Morgan

Article

Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

Insert

NOS2 (NOS2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CYBL-SPI1-CEBPB-IRF5

Plasmid

#190719

Purpose

For microglia differentiation from iPSC

Depositor

Li Gan

Article

Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

Insert

SPI1-CEBPB-IRF5

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_mScarlet-LAMP1

Plasmid

#185138

Purpose

Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

Depositor

Andrew York

Article

Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

Insert

Lysosome-associated membrane glycoprotein 1 (Lamp1 Synthetic, Rat)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

deleted amino acids 1-21 of LAMP1

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
phage UbiC NLS HA stdMCP stdHalo

Plasmid

#104999

Purpose

Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

stdMCP-stdHalo

Use

Lentiviral

Tags

HA tag, NLS, Two synonymous copies of the Halo-ta…

Expression

Bacterial and Mammalian

Mutation

Promoter

UbiC

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRK892

Plasmid

#71803

Purpose

Expression of mutated L. gasseri gusA from strong Ppgm promoter

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

Insert

gusA3

Use

Tags

Expression

Bacterial

Mutation

D573A

Promoter

Ppgm

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-ESR1

Plasmid

#116737

Purpose

Lentiviral expression of ESR1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ-dzCas9-Act3.0

Plasmid

#158414

Purpose

CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_L_EBOV

Plasmid

#103052

Purpose

Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

L gene of Ebola virus (L Zaire ebolavirus, Mayinga)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcs2-GFP-IRES-mCherry

Plasmid

#226098

Purpose

Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161926

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

rTetR(SE-G72P)

Use

Lentiviral and Synthetic Biology

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge