pcDNA3 myc CIITA (P#808)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14650||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameclass II transactivator
SpeciesH. sapiens (human)
Entrez GeneCIITA (a.k.a. C2TA, CIITAIV, MHC2TA, NLRA)
/ Fusion Protein
- Myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that mutations Q21E, V176M, S1023A in CIITA were found during Addgene's quality control process compared to GenBank: AAA88861.1. These mutations should not affect plasmid function, as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 myc CIITA (P#808) was a gift from Matija Peterlin (Addgene plasmid # 14650 ; http://n2t.net/addgene:14650 ; RRID:Addgene_14650)
For your References section:Tat competes with CIITA for the binding to P-TEFb and blocks the expression of MHC class II genes in HIV infection. Kanazawa S, Okamoto T, Peterlin BM. Immunity. 2000 Jan . 12(1):61-70. 10.1016/S1074-7613(00)80159-4 PubMed 10661406