pCol2 flu CIITA (P#294)
(Plasmid
#14651)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14651 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCol2
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCIITA
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Alt nameclass II transactivator
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SpeciesH. sapiens (human)
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Insert Size (bp)4500
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Entrez GeneCIITA (a.k.a. C2TA, CIITAIV, MHC2TA, NLRA)
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Tag
/ Fusion Protein
- flu
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Collagen II promoter
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCol2 flu CIITA (P#294) was a gift from Matija Peterlin (Addgene plasmid # 14651 ; http://n2t.net/addgene:14651 ; RRID:Addgene_14651) -
For your References section:
Tat competes with CIITA for the binding to P-TEFb and blocks the expression of MHC class II genes in HIV infection. Kanazawa S, Okamoto T, Peterlin BM. Immunity. 2000 Jan . 12(1):61-70. 10.1016/S1074-7613(00)80159-4 PubMed 10661406
Map uploaded by the depositor.
