Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38141||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepCMV-Tag 2b
- Backbone size w/o insert (bp) 4300
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
SpeciesH. sapiens (human)
Insert Size (bp)1900
Entrez GeneNLRP10 (a.k.a. CLR11.1, NALP10, NOD8, PAN5, PYNOD)
- Promoter CMV
/ Fusion Protein
- FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer t3
- 3′ sequencing primer t7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FLAG-NLRP10 was a gift from Thomas Kufer (Addgene plasmid # 38141 ; http://n2t.net/addgene:38141 ; RRID:Addgene_38141)
For your References section:NLRP10 enhances Shigella-induced pro-inflammatory responses. Lautz K, Damm A, Menning M, Wenger J, Adam AC, Zigrino P, Kremmer E, Kufer TA. Cell Microbiol. 2012 Jun 4. doi: 10.1111/j.1462-5822.2012.01822.x. 10.1111/j.1462-5822.2012.01822.x PubMed 22672233