165,744 results

1. pBIG2abcde

   Plasmid

   #80619

   Purpose

   pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIG2abcd

   Plasmid

   #80618

   Purpose

   pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

3. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

   Plasmid

   #208954

   Purpose

   A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A), Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBIG1c

   Plasmid

   #80613

   Purpose

   pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAF0443 Nm60_attP-mCherry donor

   Plasmid

   #193471

   Purpose

   Promoterless mcherry donor for Nm60

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Nm60_attP
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIG1d

   Plasmid

   #80614

   Purpose

   pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBIG1e

   Plasmid

   #80615

   Purpose

   pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. TOP-H2B-mCherry-DD

   Plasmid

   #96894

   Purpose

   Reporter to monitor translation of 5'TOP mRNA

   Depositor

   Tobias Meyer

   Article

   Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

   Insert

   TOP-H2B-mCherry-DD
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-WASH1-N-18

   Plasmid

   #55163

   Purpose

   Localization: Cytoskeleton, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   WASH1
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAF0466 Ef1a-Nm60_attB acceptor

    Plasmid

    #193476

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK01

    Plasmid

    #64744

    Purpose

    Yeast expression plasmid: phaA, phaB, phaC

    Depositor

    Jens Nielsen

    Article

    Kocharin et al AMB Express. 2012 Sep 25;2(1):52. doi: 1

    Insert

    phaA, phaB, phaC
    

    Expression

    Yeast

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pEAK10-His-Myc-Kal7

    Plasmid

    #25454

    Depositor

    Betty Eipper

    Article

    Penzes et al J Biol Chem. 2000 Mar 3. 275(9):6395-403

    Insert

    Kal7
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR v2-sgDHODH-4

    Plasmid

    #186022

    Purpose

    knock out DHODH in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRUPATH Triple Ga13

    Plasmid

    #196062

    Purpose

    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET30b-5M SrtA with TEV site

    Plasmid

    #86962

    Purpose

    Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

    Depositor

    Ron Bose

    Article

    Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

    Insert

    S.aureus SrtA 5M
    

    Tags

    6x Histidine tag and TEV site

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

    Plasmid

    #216319

    Purpose

    Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Cerulean + splice acceptor site
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

    Plasmid

    #216318

    Purpose

    Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    split cerulean fluorescent protein + splice donor site
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIA1414

    Plasmid

    #172402

    Purpose

    Expression of SARS-COV-2 Nsp9 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp9 (ORF1ab Synthetic)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ubi:ccdb-eGFP + RUBY

    Plasmid

    #233310

    Purpose

    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb-eGFP + RUBY
    

    Use

    Gateway destination

    Tags

    eGFP

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ubi:ccdb + RUBY

    Plasmid

    #233306

    Purpose

    GATEWAY compatible vector with soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb + RUBY
    

    Use

    Gateway destination

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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