163,517 results

1. PMP34-mCherry-EGFP-Tet-On

   Plasmid

   #109017

   Purpose

   Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   PMP34 (SLC25A17 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1906

   Plasmid

   #141883

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF7L2 (TCF7L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK2014-AaCas12b

   Plasmid

   #121949

   Purpose

   E. coli expression, protein purification

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b
   

   Use

   Tags

   10xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPROBE-GT

   Plasmid

   #37814

   Depositor

   Steven Lindow

   Article

   Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   Promotorless gfp reporter gene
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pTN4

   Plasmid

   #174212

   Purpose

   Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Inserts

   AtTIR1(F79G)::mRuby (TIR1 Nematode)
   GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   mRuby

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. RNASEL gRNA (BRDN0001149212)

   Plasmid

   #77051

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RNASEL

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RNASEL (RNASEL Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. RNASEL gRNA (BRDN0001149358)

   Plasmid

   #77052

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RNASEL

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RNASEL (RNASEL Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IP-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #84572

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDC-UAS-nBitbow1.0(miniW)

   Plasmid

   #138329

   Purpose

   Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.

   Depositor

   Dawen Cai

   Article

   Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3

   Inserts

   mAmetrine
   tdKatushka2
   mNeonGreen
   mTFP1
   mKusabira-Orange2
   

   Use

   Tags

   human H2B

   Expression

   Insect

   Mutation

   Promoter

   10xUAS

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-8:TOMM20-mEGFP

    Plasmid

    #87423

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBABE puro PPAR delta

    Plasmid

    #8891

    Depositor

    Bruce Spiegelman

    Article

    Brun et al Genes Dev. 1996 Apr 15. 10(8):974-84.

    Insert

    PPAR delta (Ppard Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-Neo-ISG15

    Plasmid

    #80404

    Purpose

    overexpression of ISG15

    Depositor

    Isidoro Martinez

    Article

    Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-Gsdmd-CT

    Plasmid

    #80953

    Purpose

    Expresses Gsdmd-CT in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

    Insert

    Gsdmd-CT (Gsdmd Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB150

    Plasmid

    #101661

    Purpose

    Opto-T7RNAP*(563)

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(563)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

    Plasmid

    #196971

    Purpose

    Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

    Depositor

    Leopold Parts

    Inserts

    Cas9-RT
    rtTA3G-P2A-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and TREG3G

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-HA-SZT2

    Plasmid

    #87035

    Purpose

    mammalian expression of SZT2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

    Plasmid

    #214994

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTCF5 HLA-A*01:01

    Plasmid

    #180448

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*01:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHIV-EGFP.Flag-EZH2

    Plasmid

    #220243

    Purpose

    Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6xHis-MBP-huBsCas12a

    Plasmid

    #174671

    Purpose

    Expresses 6xHis-MBP tagged BsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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