167,150 results
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Plasmid#182884PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertAzoRS-4
ExpressionBacterialAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES eGFP
Plasmid#128059PurposeLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN(deltaNLS)-pmGFP
Plasmid#26867PurposeExpresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membraneDepositorInsertCIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
TagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterCMVAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (KmR)
Plasmid#177207PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
ExpressionBacterialPromoterSynthetic constitutive promoterAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-STING_gRNA3
Plasmid#127640PurposeKnock-out of human STINGDepositorInsertSTING (STING1 Human)
UseLentiviralAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2
Plasmid#202566PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopyDepositorInsertLINE-1
TagsmNeonGreen2 on ORF1pExpressionMammalianAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-Flex-GFP-Fishell_6
Plasmid#83895PurposeCre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV8, and AAV9InserteGFP
UseAAV and Cre/LoxExpressionMammalianPromoterhDlxAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_erGAP3
Plasmid#78118PurposeLow affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulumDepositorInserterGAP3
TagsKDEL and calreticulin signal peptideExpressionMammalianMutationS175G, D180Y, I167V and L15Q in GFP moiety and D…PromoterCMVAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA32 (MSW#497)
Plasmid#208068PurposeExpression of GFP-tagged hsRPA2 in human cellsDepositorAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA14 (MSW#503)
Plasmid#208069PurposeExpression of GFP-tagged hsRPA3 in human cellsDepositorAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-VSVgSS-TM
Plasmid#207320PurposeAllows cloning of protein coding sequences to be incorportated into envelope of virionsDepositorTypeEmpty backboneExpressionMammalianAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO MYC3
Plasmid#177848PurposeBacterial Expression of MYC3DepositorAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_miRFP670
Plasmid#113726PurposeExpresses the fluorescent protein miRFP670 in a lentiviral backboneDepositorInsertmiRFP670
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 Neo
Plasmid#127644PurposeEmpty pLentiCRISPRv2 with NeoRDepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-SMC3
Plasmid#222554PurposePiggyBac transposon plasmid for doxycycline inducible expression of SMC3DepositorInsertSMC3 (SMC3 Human)
ExpressionMammalianAvailable SinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWRS3001_VCR1_WCR3
Plasmid#192206PurposeVCR1 and WCR3 tracrRNAs cloned into pTWIST AMP high copy backboneDepositorInsertVCR1-WCR3 tracrRNA
ExpressionMammalianAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-msfGFPBb
Plasmid#118231PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFPDepositorInsertflagellin promoter driven msfGFP
ExpressionBacterialAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1
Plasmid#20061PurposeBacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formationDepositorInsertMSP1D1
Tags7-His tagExpressionBacterialMutationdeletion mutant (1-11) of MSP1; His-tag on N-term…Available SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only