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pLV-mScarlet-Control-5'UTR

Plasmid

#184637

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Expression

Mammalian

Available Since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRDA_112

Plasmid

#136475

Purpose

Lentiviral expression of AsCas12a

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

AsCas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pDESTN-MTp-p53-Venus

Plasmid

#53736

Purpose

Expresses Venus tagged p53 under the metallothionein promoter

Depositor

Galit Lahav

Article

Batchelor et al Mol Cell. 2008 May 9;30(3):277-89. doi:

Insert

p53 (TP53 Human)

Tags

Venus

Expression

Mammalian

Promoter

metallothionein promoter

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hGfap-R-CaMP1.07-WPRE-SV40

Plasmid

#164140

Purpose

AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

Depositor

Hirokazu Hirai, Hajime Hirase

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

R-CaMP1.07

Use

AAV

Promoter

hGFAP

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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HIF-TRE-mStrawberry reporter

Plasmid

#158681

Purpose

This plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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Mitochondrial PancACe

Plasmid

#215710

Purpose

Expresses acetyl-CoA biosensor in the mitochondria

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-EGFP-PiggyBac

Plasmid

#190003

Purpose

Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

Depositor

Tineke Lenstra

Article

Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

Insert

MCP-EGFP

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-Flavidin

Plasmid

#89881

Purpose

Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K80R, K121R, K132R, K134R

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-p300 (D1399Y)

Plasmid

#191763

Purpose

Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

FRB-EGFP-p300 core (D1399Y) (EP300 Human)

Tags

EGFP (N-terminal of p300 core (D1399Y)) and FRB (…

Expression

Bacterial and Mammalian

Mutation

catalytic D1399Y mutation in p300

Promoter

EF1alpha

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV SNAP MDC1

Plasmid

#227375

Purpose

SNAP–MDC1 expression

Depositor

David Pellman

Article

Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

Insert

MDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA (MSW#111)

Plasmid

#208066

Purpose

expression of human Replication Protein A in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)

Expression

Bacterial

Mutation

RPA32 has a point mutation Thr88(ACC) -> Ile(A…

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-GFPuv

Plasmid

#127078

Purpose

Low phosphate inducible yibD gene promoter expressing GFPuv reporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 delta

Plasmid

#20785

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

pDCNA3 Flag p38 delta (Mapk13 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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hIRE1a.pcD

Plasmid

#21892

Depositor

Randal Kaufman

Insert

IRE1a (ERN1 Human)

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD3

Plasmid

#153118

Purpose

Expression of Flag tagged human FOXD3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD3 (FOXD3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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JB28

Plasmid

#128900

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab5AQL-BirA*-HA-MAO (RAB5A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCGN-MEF2D

Plasmid

#32963

Purpose

For mammalian expression of mouse MEF2D with an HA tag

Depositor

Ron Prywes

Insert

MEF2D (Mef2d Mouse)

Tags

HA

Expression

Mammalian

Mutation

E287G

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pEFS-ccdB-12X MS2_mPGK-Puro

Plasmid

#177809

Purpose

Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

Depositor

Sven Eyckerman

Article

Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

Type

Empty backbone

Tags

12X MS2 stem loops

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Chrm3_Csx30_Cre

Plasmid

#197603

Purpose

Cre reporter for mammalian cells with Chrm3 membrane anchor

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Chrm3_Csx30_Cre

Expression

Mammalian

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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p11d-aRPA (MSW#712)

Plasmid

#208078

Purpose

Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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