165,750 results

1. phU6-gRNA

   Plasmid

   #53188

   Purpose

   Expresses the S. pyogenes sgRNA from the human U6 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Arl13b-mcherry

   Plasmid

   #234845

   Purpose

   A mammalian expression plasmid encoding Arl13b with mcherry at C-terminus.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Arl13b (ARL13B Human)
   

   Expression

   Mammalian

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Red Glifon 3000

   Plasmid

   #163116

   Purpose

   Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

   Depositor

   Takashi Tsuboi

   Article

   Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

   Insert

   Red Glifon 3000
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PiggyBac dCas9-VPR

   Plasmid

   #191269

   Purpose

   piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Inserts

   VPR-dCas9-HA-tagBFP
   rtTA3-IRES-PURO
   

   Use

   CRISPR

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

   Plasmid

   #215769

   Purpose

   encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

   Depositor

   Dimitrios Wagner

   Article

   Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

   Inserts

   TRAC-5' homology arm (TRAC Human)
   P2A-CD19.28.zeta-bgH (CD19 Mouse, Human, Bovine)
   TRAC-3' homology arm (TRAC Human)
   

   Expression

   Bacterial

   Mutation

   Cas12a PAM mutated

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mAzurite-16xRhoBAST

   Plasmid

   #196347

   Purpose

   Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

   Depositor

   Murat Sunbul

   Article

   Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

   Insert

   mAzurite-16xRhoBAST
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-HP1alpha

   Plasmid

   #17652

   Depositor

   Tom Misteli

   Article

   Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

   Insert

   HP1 alpha (CBX5 Human)
   

   Tags

   GFP and His

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV pCAG-FLEX-EGFP-WPRE

   Plasmid

   #51502

   Purpose

   Can be used to generate AAV virus that will express EGFP in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET GFP LIC cloning vector (2GFP-T)

   Plasmid

   #29716

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, SiriusGFP, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. 4xmts-mScarlet-I

    Plasmid

    #98818

    Purpose

    In vivo visualization of mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    mts
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CMV-MCS-Puro

    Plasmid

    #219794

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hsp90 HA

    Plasmid

    #22487

    Depositor

    William Sessa

    Article

    Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

    Insert

    Hsp90 HA (HSP90AB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHaMDRwt

    Plasmid

    #10957

    Depositor

    Michael Gottesman

    Article

    Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(

    Insert

    MDR1 (ABCB1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTi4.0_SpdCas9_pmcDA1

    Plasmid

    #204613

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-GINKO2

    Plasmid

    #177116

    Purpose

    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

    Depositor

    Robert Campbell

    Article

    Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

    Insert

    GINKO2
    

    Tags

    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3- SREBP-1

    Plasmid

    #204192

    Purpose

    Mammalian expression of SREBP-1 (isoform 1)

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-1 (SREBF1 Human)
    

    Tags

    E-tag and T7x2

    Expression

    Mammalian

    Mutation

    multiple silent mutations

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pgRNA-humanized

    Plasmid

    #44248

    Purpose

    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pH2B-emiRFP670

    Plasmid

    #136571

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    H2B-emiRFP670
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep

    Plasmid

    #173899

    Purpose

    The pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThr

    Depositor

    Jason W Chin

    Article

    Zhang et al Nat Methods. 2017 Jul;14(7):729-736. doi

    Inserts

    ThrRS(v1.0)
    tRNA(v2.0)CUA
    pduX
    EF-Sep
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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