PurposeIn vivo visualization of lysosomes (can be used for colocalization studies)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||98827||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- Promoter CMV
/ Fusion Protein
- mScarlet-I (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/early/2017/07/06/160374 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lamp1-mScarlet-I was a gift from Dorus Gadella (Addgene plasmid # 98827 ; http://n2t.net/addgene:98827 ; RRID:Addgene_98827)
For your References section:Robust and Bright Genetically Encoded Fluorescent Markers for Highlighting Structures and Compartments in Mammalian Cells. Chertkova AO, Mastop M, Postma M, van Bommel N, van der Niet S, Batenburg KL, Joosen L, Gadella TWJ, Okada Y, Goedhart J. bioRxiv 160374 10.1101/160374