3544 pMIG Bcl-2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8793||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)720
Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
Note that the GFP in this plasmid is not fused to gene. It is for selection purposes only.
Please note that this plasmid is an extremely slow grower and may take up to 3 days to grow.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:3544 pMIG Bcl-2 was a gift from Stanley Korsmeyer (Addgene plasmid # 8793 ; http://n2t.net/addgene:8793 ; RRID:Addgene_8793)
For your References section:BCL-2, BCL-X(L) sequester BH3 domain-only molecules preventing BAX- and BAK-mediated mitochondrial apoptosis. Cheng EH, Wei MC, Weiler S, Flavell RA, Mak TW, Lindsten T, Korsmeyer SJ. Mol Cell 2001 Sep;8(3):705-11. 10.1016/S1097-2765(01)00320-3 PubMed 11583631