165,343 results

1. pTriEx-NLyn-Venus-Zdk1

   Plasmid

   #81042

   Purpose

   mammalian expression of plasma-membrane anchored Zdk1

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk1
   

   Tags

   6x His tag, FLAG, NLyn, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV2_HLF-ZP

   Plasmid

   #175035

   Purpose

   HLF targeting construct introducing an ires ZsG P2A Puro cassette downstream of the human HLF stop codon

   Depositor

   Guy Sauvageau

   Article

   Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

   Insert

   HLF ires ZsGreen P2A Puro
   

   Use

   AAV

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVITRO1-mCardinal-CAXX

   Plasmid

   #184451

   Purpose

   Plasma membrane-targeted mCardinal expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   mCardinal-CAAX
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX6p-1-UFBP1

   Plasmid

   #204558

   Purpose

   Expresses GST-tagged UFBP1 in E.coli

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   UFBP1 (DDRGK1 Human)
   

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHelper_NoMutL_V2_gentR

   Plasmid

   #205293

   Purpose

   Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Insert

   CspRecT / Bxb-1
   

   Use

   Synthetic Biology

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR-BsaI

   Plasmid

   #54340

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Type

   Empty backbone

   Use

   Entry vector

   Available Since

   March 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE2-TetOminiCMV-mCherry

   Plasmid

   #136615

   Purpose

   Dox-inducible lentivral vector expressing mCherry red fluorescent protein

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Bacterial

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tet-O-FUW-FOSL2-P2A-mCherry

   Plasmid

   #203860

   Purpose

   Tet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   FOSL2 (FOSL2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

   Plasmid

   #35511

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSET FLIPPi-5u

    Plasmid

    #13554

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi S52A
    

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    S52A in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Halo-DNAPKcs HRD

    Plasmid

    #207088

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Rc/CMV cyclin C

    Plasmid

    #8961

    Depositor

    Bob Weinberg

    Article

    Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

    Insert

    cyclin C (CCNC Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5FRT/TO-p47-Strep-HA

    Plasmid

    #113475

    Purpose

    Expression of human p47 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    p47 (NSFL1C Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUdO1k

    Plasmid

    #210234

    Purpose

    high-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEAT8-137 M1V

    Plasmid

    #173807

    Purpose

    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

    Depositor

    Anne Gershenson

    Article

    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

    Insert

    SERPiNA1 (SERPINA1 Human)
    

    Expression

    Bacterial

    Mutation

    E1M Otherwise this plasmid encodes the most commo…

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMRX-IZ-HaloTag7-Galectin3

    Plasmid

    #184896

    Purpose

    Stable expression of HaloTag7-Galectin3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    Galectin3
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 8522-M31-663

    Plasmid

    #225661

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCherry
    

    Use

    Lentiviral

    Promoter

    SORE6-mCMVp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCIG PIK3CA Wildtype

    Plasmid

    #73056

    Purpose

    Human PIK3CA Wildtype

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    PIK3CA Wildtype (PIK3CA Human)
    

    Expression

    Mammalian

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. huntingtin_FL_NbioCflag

    Plasmid

    #194767

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pcDNA3.1(-)-HA-SrtAstrep (pKS77)

    Plasmid

    #50030

    Purpose

    Expression of cytosolic S. pyogenes SrtA in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    SrtA(strep)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge