159,258 results

1. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

   Plasmid

   #232929

   Purpose

   Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   OsTir1(F74G)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   changed Phenylalanine 74 to Glycine

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGEN-PspCas13b-NES-mCherry

   Plasmid

   #233608

   Purpose

   Expression of PspCas13b in mammalian cells

   Depositor

   Shintaro Iwasaki

   Article

   Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

   Insert

   PspCas13b-NES-mCherry
   

   Use

   CRISPR

   Tags

   3xFlag and mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pC016-gPsp-EGFP_start_codon

   Plasmid

   #233613

   Purpose

   Expression of guide RNA targeting the start codon of the EGFP gene for PspCas13b

   Depositor

   Shintaro Iwasaki

   Article

   Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

   Insert

   gRNA targeting EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)

   Plasmid

   #221329

   Purpose

   Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   FLAG-IRSp53 and HA-Cdc42(G12V) (Baiap2, Cdc42 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   G12V

   Promoter

   TRE3G

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR Ef1a-dCas9-BFP-KRAB

   Plasmid

   #217304

   Purpose

   lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

   Depositor

   Luke Gilbert

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Ef1alpha

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE-Mch-MAP1LC3B

   Plasmid

   #201924

   Purpose

   For lentiviral transduction of Mch-MAP1LC3B

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Pan-QKI scFv [N147/6]

   Plasmid

   #211605

   Purpose

   Mammalian Expression Plasmid of Pan-QKI scFv. Derived from hybridoma N147/6.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Pan-QKI (Homo sapiens) recombinant mouse scFv. (QKI Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG/ATP10D(E215Q)-HA

   Plasmid

   #209248

   Purpose

   Mammalian expression of ATP10D mutant E215Q

   Depositor

   Hye-Won Shin

   Article

   Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

   Insert

   ATP10D (ATP10D Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E215Q

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQCXIZ GFP PODXL delta IC

   Plasmid

   #202422

   Purpose

   Expression of GFP-tagged PODXL without intracellular domain

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   deleted intracellular domain

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQCXIZ GFP PODXL delta PBM + Ezrin

    Plasmid

    #202418

    Purpose

    Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    Ezrin and GFP

    Expression

    Mutation

    delete PDZ binding motif

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRA1EGFP

    Plasmid

    #193791

    Purpose

    Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PR promoter

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pD649-HAsp-NOTCH2(Trunc1)-COMP5AP-AviTag-9xHis

    Plasmid

    #157611

    Purpose

    Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NOTCH2 (NOTCH2 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pD649-HAsp-SEMA7A-COMP5AP-AviTag-9xHis

    Plasmid

    #157486

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA7A (SEMA7A Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pD649-HAsp-JAG2-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #157027

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    JAG2 (JAG2 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Aldh1L1 scFv [N103/31]

    Plasmid

    #190522

    Purpose

    Mammalian Expression of Aldh1L1 scFV. Derived from hybridoma N103/31.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Aldh1L1 (Rattus norvegicus) recombinant scFV (Aldh1l1 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p5xQUAS-ZsGreen-P2A, α-cry:mCherry

    Plasmid

    #180477

    Purpose

    Inducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherry

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Inserts

    5x QUAS-ZsGreen-P2A
    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Epac-S-H170

    Plasmid

    #170352

    Purpose

    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

    Insert

    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHCKan-yibDp-ald*-alaE

    Plasmid

    #134939

    Purpose

    Low phosphate inducible alanine dehydrogenase and alanine exporter

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    alanine dehydrogenase and L-alanine exporter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R777-E202 Hs.PRKAG2-nostop

    Plasmid

    #70486

    Purpose

    Gateway ORF clone of human PRKAG2 [NM_024429.1] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PRKAG2 (PRKAG2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p414GPD_Hsp82viiB

    Plasmid

    #61709

    Purpose

    Yeast plasmid with Hsp82 heterodimer construct.

    Depositor

    Dan Bolon

    Article

    Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

    Insert

    Hsp82viiB (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Heteromeric coiled coil inserted after amino acid…

    Promoter

    GPD1

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge