159,238 results

1. pTS395_PhCMV-SB100

   Plasmid

   #169633

   Purpose

   Expression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven SB100
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PGK-Venus-Fluc (puro)

   Plasmid

   #140328

   Purpose

   Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

   Depositor

   Roland Friedel

   Article

   Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

   Insert

   Venus-Firefly Luciferase (luc2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   human PGK promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CL20_mEGFP_CBFB_MYH11

   Plasmid

   #205787

   Purpose

   Express mEGFP-tagged fusion protein, CBFB_MYH11 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   CBFB_MYH11 (CBFB, MYH11 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lentiviral-S-tdTomato

   Plasmid

   #112579

   Purpose

   Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

   Depositor

   Jeffrey Rothstein

   Article

   Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

   Insert

   NLS-tdTomato-NES
   

   Use

   Lentiviral

   Tags

   nuclear export sequence and nuclear localization …

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. myc-KIF5B

   Plasmid

   #127617

   Purpose

   N-terminal myc-tagged mouse KIF5B

   Depositor

   Josef Kittler

   Article

   Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-7 asyn E46K

   Plasmid

   #105728

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein E46K (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E46K

   Promoter

   T7

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHSN6I01

   Plasmid

   #50587

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   dCas9-KRAB
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG, HA, and NLS

   Expression

   Mutation

   dCas9 that is defective in DNA cleavage; the maiz…

   Promoter

   2×35Sp and AtU6-26p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-hARΔ142–337

   Plasmid

   #89106

   Purpose

   Mammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1

   Depositor

   Elizabeth Wilson

   Article

   Titus et al PLoS One. 2012;7(1):e30192. doi: 10.1371

   Insert

   hAR delta 142-337 (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR delta 142-337

   Promoter

   CMV

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

9. AAV hSYN DIO mGluR1-myc-FLAG

   Plasmid

   #166227

   Purpose

   AAV expressing cre-dependent mGluR1-myc-FLAG

   Depositor

   Jason Aoto

   Article

   Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

   Insert

   metabotropic glutamate receptor 1 (Grm1 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSR43.6r

    Plasmid

    #63197

    Purpose

    Expresses CcaS, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER21-ERG

    Plasmid

    #51301

    Purpose

    Expression of ERG after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    v-ets avian erythroblastosis virus E26 oncogene homolog (ERG Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    full length ORF

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCerulean

    Plasmid

    #24756

    Depositor

    Martin Fraunholz

    Article

    Paprotka et al J Microbiol Methods. 2010 Aug 10. ():.

    Insert

    Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-hARdelta120-472

    Plasmid

    #89088

    Purpose

    mammalian expression of human androgen receptor with deletion of 120-472 amino acid residues

    Depositor

    Elizabeth Wilson

    Article

    Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

    Insert

    hAR-120-472 N-term deletion (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR-120-472

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-hAR1-660

    Plasmid

    #89079

    Purpose

    mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids

    Depositor

    Elizabeth Wilson

    Article

    Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

    Insert

    hAR-1-660 (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 1-660 (contains exons A,B,C and part of D)

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX307 P2A GFP YAP1

    Plasmid

    #193675

    Purpose

    Constitutive lentiviral expression of YAP1

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pILGFPB5A

    Plasmid

    #83556

    Purpose

    Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    GAL1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-eNme2-C-ABE8e

    Plasmid

    #185667

    Purpose

    Expresses eNme2-C-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-M4-AR-S81A

    Plasmid

    #171241

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Ser81Ala (AR-S81A)

    Promoter

    CMV

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. hNFAT2-409:TALE:VP64:KRɸ

    Plasmid

    #160661

    Purpose

    Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    hNFAT2-409:TALE:VP64:KRɸ
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5FRT/Flag-WDR5

    Plasmid

    #29759

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    WDR5 (WDR5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2013

    Availability

    Academic Institutions and Nonprofits only