Addgene

Showing: 2341 - 2360 of 157452 results

Results per page:

Sort results by:

pAAV-hSyn1-FLEX-CybSEP2-WPRE

Plasmid

#207661

Purpose

Monitoring neuropeptide release

Depositor

Sung Han

Article

Kim et al bioRxiv 2023.01.19.524797

Insert

Cytochrome b-561 (Cyb561 Mouse)

Use

AAV

Tags

Expression

Mutation

Histidine 86 and 159 to alanines, inserted 2 X SE…

Promoter

hSynapsin

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EXT1-pLJC2-3XFLAG

Plasmid

#215818

Purpose

Ectopic expression of EXT-1 in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET-MagLOV

Plasmid

#219957

Purpose

Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

Depositor

Andrew York

Article

Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Insert

MagLOV

Use

Tags

6xHis

Expression

Bacterial

Mutation

AsLOV2 C450P L496V Q513K G528K D540M

Promoter

T7

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-DHX36iso1

Plasmid

#159585

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-mgSrtA

Plasmid

#125792

Purpose

Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

mgSrtA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR3.1-mGM-CSF

Plasmid

#74465

Purpose

expression of mGM-CSF in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSyn.iGluSnFR3.v857.IgK-NGR

Plasmid

#196227

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV hSyn iGluSnFR3 v857.NGR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and NGR TM Domain

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2olig-TDP-43

Plasmid

#218920

Purpose

Expression of CRY2olig-mCherry fused to TDP-43

Depositor

Tatyana Shelkovnikova

Insert

CRY2olig-mCherry tagged TDP-43

Use

Tags

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Nsp3 -EGFP

Plasmid

#165108

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp3 (ORF1ab SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

Plasmid

#216533

Purpose

An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

Depositor

Yuki Kambe

Article

Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

Insert

axon-jGCaMP8m

Use

AAV and Cre/Lox

Tags

GAP43

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-Spike

Plasmid

#145031

Purpose

Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

SARS-CoV Spike

Use

Tags

C9 and CD5 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
cyto-iATPSnFR1.0

Plasmid

#102550

Purpose

cytoplasmic ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXN22-Dest

Plasmid

#105085

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Use

Tags

NubG-3xHA

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR4A1-donor

Plasmid

#112295

Purpose

CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP

Depositor

Kevin White

Insert

NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)

Use

CRISPR

Tags

Expression

Mutation

hg38:12:52058840:C>T, hg38:12:52058930:A>C,…

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SPS-6803

Plasmid

#80458

Purpose

IPTG inducible expressoin of sucrose phosphate synthase derived from Synechocystis sp. PCC6803

Depositor

Daniel Ducat

Article

Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

Insert

Sucrose phosphate synthase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
fluoPEER-HEK3_5bp-del

Plasmid

#185476

Purpose

Fluorescent reporter for a 5 basepair deletion at the HEK3 locus.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

HEK3 editing site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p4xmts-mScarlet3_N1

Plasmid

#189774

Purpose

Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

4xmts-mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPRi(v2)-Blast

Plasmid

#170068

Purpose

Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9-MeCP2

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-MRLC1

Plasmid

#35680

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only