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lenti-sgRNA puro

Plasmid

#104990

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pAAV-mDlx-ChR2-mCherry-Fishell-3

Plasmid

#83898

Purpose

ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Publication

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has service

AAV Retrograde, AAV1, and AAV9

Insert

ChR2

Use

AAV

Expression

Mammalian

Promoter

mDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCW57-RFP-P2A-MCS

Plasmid

#78933

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Publication

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

Tight TRE promoter

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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mEos4b-N1

Plasmid

#54814

Purpose

Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Publication

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4b

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pAAV-hSyn-DIO-EGFP

Plasmid

#50457

Purpose

Double floxed EGFP under the control of human synapsin promoter

Depositor

Bryan Roth

Has service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EGFP

Use

AAV

Tags

N/A

Promoter

human Synapsin 1

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

123 Articles

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pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Plasmid

#41863

Purpose

This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

Depositor

Feng Zhang

Publication

Cong et al Science. 2013 Jan 3.

Insert

U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ac5-STABLE1-neo

Plasmid

#32425

Depositor

Rosa Barrio, James Sutherland

Publication

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Insert

GFP-T2A-neo

Expression

Insect

Promoter

Actin5c

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pBPnlsLexA::p65Uw

Plasmid

#26230

Depositor

Gerald Rubin

Publication

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::p65 (RELA Human)

Expression

Insect

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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CD44P pGL3

Plasmid

#19122

Depositor

Bob Weinberg

Publication

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 promoter (CD44 Human)

Use

Non viral cloning vector

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCL-CTIG

Plasmid

#14901

Purpose

3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

Depositor

Inder Verma

Publication

Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

Insert

CMV-tTA-GFP

Use

Lentiviral

Expression

Mammalian

Available since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV5 human p27

Plasmid

#14049

Depositor

Joan Massague

Publication

Luo et al Nature. 1995 May 11. 375(6527):159-61.

Insert

p27 (CDKN1B Human)

Expression

Mammalian

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMVTNT PINK1 C-myc

Plasmid

#13314

Depositor

Mark Cookson

Publication

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

myc

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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HA-cdh1

Plasmid

#11596

Depositor

Marc Kirschner

Publication

Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

Insert

cdh1 (FZR1 Human)

Tags

HA

Expression

Mammalian

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pcDNA3-cdk5GFP

Plasmid

#1346

Depositor

Li-Huei Tsai

Insert

CDK5 (CDK5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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His6-MBP-uTEV3

Plasmid

#135464

Purpose

Bacterial expression of uTEV3 (full-length) protease

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

His6-MBP-uTEV3

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation (improves stability) , I138T/S153N…

Promoter

T5

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LifeAct-mNeonGreen

Plasmid

#98877

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

LifeAct

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pUC57-TPNOX

Plasmid

#87853

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Publication

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

TPNOX

Tags

C-terminal FLAG tag with a linker

Expression

Bacterial

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLX_311-Cas9

Plasmid

#96924

Purpose

for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

Depositor

John Doench, William Hahn, David Root

Publication

Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

Insert

Cas9

Use

Lentiviral

Tags

3xFlag and V5

Expression

Mammalian

Promoter

EF1a

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pFC332

Plasmid

#87845

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

Depositor

Uffe Mortensen

Publication

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
hph (hygromycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pJL-TRBO-G

Plasmid

#80083

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.

Depositor

John Lindbo

Publication

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Insert

GFP

Expression

Plant

Promoter

CaMV35S

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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