165,214 results

1. pBABE-puro-GFP-wt-lamin A

   Plasmid

   #17662

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Cell Biol. 2008 Apr;10(4):452-9. doi

   Insert

   GFP-lamin A (LMNA Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. ER-GCaMP6-210

   Plasmid

   #86919

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-210
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PEmax-P2A-GFP

   Plasmid

   #180020

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-EGFP
   

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SF-SON IRES Puro

   Plasmid

   #214402

   Purpose

   Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

   Depositor

   Tanja Kuhlmann

   Article

   Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-HDAC4

   Plasmid

   #45636

   Depositor

   Tso-Pang Yao

   Article

   Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

   Insert

   HDAC4 (HDAC4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA GFP10-ZIP

   Plasmid

   #182241

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP
   

   Tags

   split-GFP10 tripartite

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-E-Syt2

   Plasmid

   #66831

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt2 (ESYT2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA ZIP-GFP11

   Plasmid

   #182242

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP11

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   ZIP-GFP11
   

   Tags

   split-GFP11 tripartite

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHN764

   Plasmid

   #178819

   Purpose

   Expression of EndoH in E. coli

   Depositor

   Kazuhiro Abe

   Insert

   synthetic gene encoding endo-β-N-acetylglucosaminidase H
   

   Tags

   HIs8 and MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-EF1a-mCherry-EGFP-LC3B

    Plasmid

    #170446

    Purpose

    Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    mCherry-GFP-LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-AR V7

    Plasmid

    #86856

    Purpose

    Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

    Depositor

    Marco Marcelli, Michael Mancini

    Article

    Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Alternative splice variant 7 (alteration/deletion…

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcGFP1-N1-Dsup

    Plasmid

    #90020

    Purpose

    To express GFP-tagged Dsup in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. ClopHensorN

    Plasmid

    #50758

    Purpose

    Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

    Depositor

    Colin Akerman

    Article

    Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

    Insert

    ClopHensorN
    

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Promoter

    CAG

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. LAMP1-mGFP

    Plasmid

    #34831

    Depositor

    Esteban Dell'Angelica

    Article

    Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

    Insert

    LAMP1 (LAMP1 Human)
    

    Tags

    GSTGSTGSTGA linker, Kozak, and mGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid

    #50461

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-mCherry (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA and mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV delta R8.2

    Plasmid

    #12263

    Purpose

    First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.

    Depositor

    Didier Trono

    Insert

    HIV-1 GAG/POL, Tat and Rev
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHCMV_Bless

    Plasmid

    #207255

    Purpose

    Mammalian expression of Baboon Endogenous Retrovirus env protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    BaboonEndogenousRetrovirus_Rless_env
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.3_SARS2_JN.1

    Plasmid

    #218678

    Purpose

    expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (JN.1)
    

    Expression

    Mammalian

    Mutation

    L455S on the basis of BA.2.86

    Promoter

    CMV

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCPRDEnv

    Plasmid

    #1732

    Purpose

    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

    Depositor

    Garry Nolan

    Insert

    Gag-Pol and Rev
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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