Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #25983)


Item Catalog # Description Quantity Price (USD)
Plasmid 25983 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    James Thomson
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
  • GenBank ID
  • Entrez Gene
    ABCG2 (a.k.a. ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, CDw388, EST157481, GOUT1, MRX, MXR, MXR-1, MXR1, UAQTL1)
  • Tags / Fusion Proteins
    • EF1-apha promoter (N terminal on backbone)
    • IRES-Neo (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (unknown if destroyed)
  • 3′ cloning site BamH I (unknown if destroyed)
  • 5′ sequencing primer EF-1a-F
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the backbone of this plasmid is larger than the reported 6.5kb (see attached diagnostic digest).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIN4-EF2-ABCG2-IRES-Neo was a gift from Ren-he Xu (Addgene plasmid # 25983 ; ; RRID:Addgene_25983)
  • For your References section:

    Lack of ABCG2 expression and side population properties in human pluripotent stem cells. Zeng H, Park JW, Guo M, Lin G, Crandall L, Compton T, Wang X, Li XJ, Chen FP, Xu RH. Stem Cells. 2009 Oct . 27(10):2435-45. 10.1002/stem.192 PubMed 19670287